慢性牙周炎伴肥胖患者血清中IL-6、IL-18表达水平的研究
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摘要
目的探究白细胞介素6(IL-6)、白细胞介素18(IL-18)在肥胖慢性牙周炎患者血清中的表达水平变化及意义。方法选取2017年9月～2018年5月在佳木斯大学附属口腔医院牙周科门诊确诊为慢性牙周炎患者15例设为NP组,另选取同期慢性牙周炎伴肥胖患者15例设为FP组。收集两组患者外周血样本并记录牙周PD、CAL,采用双抗体夹心酶联免疫吸附法(ELISA法)测定样本中的IL-6、IL-18的表达水平,分析两组间指标的差异及相关性。结果 FP组血清中IL-6、IL-18分别为(9.16±2.35)pg/ml、(325.22±98.67)pg/ml,其表达水平分别高于NP组的(6.15±2.06)pg/ml和(241.52±78.82)pg/ml,差异有统计学意义(P<0.05);FP组中PD与血清中IL-6、IL-18呈高度正相关(r=0.894,P<0.01;r=0.819,P<0.01);CAL与血清中IL-6、IL-18呈正相关(r=0.885,P<0.01,r=0.828,P<0.01);NP组中PD与血清中IL-6、IL-18也有正相关关系(r=0.842,P<0.01,r=0.728,P<0.01);CAL与血清中IL-6、IL-18呈正相关(r=0.884,P<0.01,r=0.707,P<0.01)。结论肥胖状态所导致的外周血中IL-6、IL-18水平的升高与CP的发生发展存在着密切联系。
Objective To investigate the changes and significance of interleukin-6(IL-6) and interleukin-18(IL-18) expression in serum of obese chronic periodontitis patients. Methods From September 2017 to May 2018, 15 patients with chronic periodontitis diagnosed in the periodontal clinic of Jiamusi University Hospital of Stomatology were selected as NP group, and 15 patients with chronic periodontitis and obesity were selected as FPgroup. Peripheral blood samples were collected from both groups and PD and CAL were recorded. The expression levels of IL-6 and IL-18 in the samples were determined by double antibody sandwich enzyme-linked immunosorbent assay(ELISA).The differences and correlations between the two groups were analyzed. Results The serum levels of IL-6 and IL-18 in FP group were(9.16±2.35) pg/ml and(325.22±98.67) pg/ml, respectively, and their expression levels were higher than that of NP group(6.15±2.06) pg/ml and(241.52±78.82) pg/ml, the difference was statistically significant(P<0.05); PD in the FP group was highly positively correlated with serum IL-6 and IL-18(r =0.894, P <0.01;r =0.819,P <0.01); CAL was positively correlated with serum IL-6 and IL-18(r=0.885, P<0.01, r=0.828, P<0.01);There was a positive correlation between PD and serum IL-6 and IL-18 in NP group(r=0.842,P<0.01,r=0.728, P<0.01); CAL was positively correlated with serum IL-6 and IL-18(r=0.884,P<0.01,r=0.707,P<0.01). Conclusion The increase of IL-6 and IL-18 levels in peripheral blood caused by obesity is closely related to the occurrence and development of CP.
Objective To investigate the changes and significance of interleukin-6(IL-6) and interleukin-18(IL-18) expression in serum of obese chronic periodontitis patients. Methods From September 2017 to May 2018, 15 patients with chronic periodontitis diagnosed in the periodontal clinic of Jiamusi University Hospital of Stomatology were selected as NP group, and 15 patients with chronic periodontitis and obesity were selected as FPgroup. Peripheral blood samples were collected from both groups and PD and CAL were recorded. The expression levels of IL-6 and IL-18 in the samples were determined by double antibody sandwich enzyme-linked immunosorbent assay(ELISA).The differences and correlations between the two groups were analyzed. Results The serum levels of IL-6 and IL-18 in FP group were(9.16±2.35) pg/ml and(325.22±98.67) pg/ml, respectively, and their expression levels were higher than that of NP group(6.15±2.06) pg/ml and(241.52±78.82) pg/ml, the difference was statistically significant(P<0.05); PD in the FP group was highly positively correlated with serum IL-6 and IL-18(r =0.894, P <0.01;r =0.819,P <0.01); CAL was positively correlated with serum IL-6 and IL-18(r=0.885, P<0.01, r=0.828, P<0.01);There was a positive correlation between PD and serum IL-6 and IL-18 in NP group(r=0.842,P<0.01,r=0.728, P<0.01); CAL was positively correlated with serum IL-6 and IL-18(r=0.884,P<0.01,r=0.707,P<0.01). Conclusion The increase of IL-6 and IL-18 levels in peripheral blood caused by obesity is closely related to the occurrence and development of CP.
引文
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[14]Chitrapriya MN,Rao SR,Lavu V.Interleukin-17 and interleukin-18 levels in different stages of inflammatory periodontal disease[J].J Indian Soc Periodontol,2015,19(1):14-17.
[15]闫艳梅,贺涛,刘琪.肥胖与牙周炎相互作用机制的研究进展[J].牙体牙髓牙周病学杂志,2016,26(7):443-447.
[2]Akram Z,Abduljabbar T,Hassan MIA,et al.Cytokine Profile in Chronic Periodontitis Patients with and without Obesity:A Systematic Review and Meta-Analysis[J].Disease Markers,2016(2):4801418.
[3]Coralia RH.IL-18 and Anti-P gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis[J].International Journal of Immunology,2016,4(4):20-26.
[4]Chaffee BW,Weston SJ.Association between chronic periodontal disease and obesity:a systematic review and meta-analysis[J].J Periodontol,2010,81(12):1708-1724.
[5]de CastILhos ED,Horta BL,Gigante DP,et al.Association between obesity and periodontal disease in young adults:a pulation-based birth cohort[J].J Clin Periodontol,2012,39(8):717-724.
[6]Eke PI,Page RC,Wei L,et al.Update of the case definitionsfor population-based surveillance of periodontitis[J].J Periodontol,2012,83(12):1449-1454.
[7]中华人民共和国卫生部疾病控制司.中国成人超重和肥胖症预防控制指南[M].北京:人民卫生出版社,2006.
[8]Gundala R,Chava VK,Ramalingam K.Association of leptin in periodontitis and acute myocardial infarction[J].J Perio-dontol,2014,85(7):917-924.
[9]Lana JP,Martins LB,Oliveira MC,et al.TNF and IL-18 cytokines may regulate liver fat storage under homeo-stasis conditions[J].Appl Physiol Nutr Metab,2016,41(12):1295-1302.
[10]Scapoli L,Girardi A,Palmieri A,et al.Interleukin-6 gene polymorphism modulates the risk of periodontal diseases[J].Journal of Biological Regulators&Homeostatic Agents,2015,29(3Suppl 1):111.
[11]石丽萍,何艳艳,王丽.大鼠实验性牙周炎治疗前后血清中IL-18与s TREM-1的浓度变化[J].口腔医学研究,2015,31(06):591-593.
[12]Sims JE.IL-1 and IL-18 receptors, and their extended famILy[J].CurtOpin Immunol,2012(14):117-122.
[13]Yue Y,Liu Q,Xu C,et al.Comparative evaluation of cytokine in gingival crevicular fluid and saliva of patients with aggressiveperiodontitis[J].International Journal of Biological Markers,2013,3(5):70-73.
[14]Chitrapriya MN,Rao SR,Lavu V.Interleukin-17 and interleukin-18 levels in different stages of inflammatory periodontal disease[J].J Indian Soc Periodontol,2015,19(1):14-17.
[15]闫艳梅,贺涛,刘琪.肥胖与牙周炎相互作用机制的研究进展[J].牙体牙髓牙周病学杂志,2016,26(7):443-447.