解淀粉芽孢杆菌产纤维素酶及其酶解能力相关研究进展
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摘要
纤维素是地球上丰富的天然生物聚合物,是重要的可再生有机资源,在农作物秸秆中尤为丰富。纤维素、半纤维素和木质素混合组成木质纤维素,木质纤维素结构的复杂性限制自然界中大量秸秆的降解。纤维素酶是将纤维素降解成葡萄糖的一类酶的总称,主要作用于纤维素及其衍生物,在木质纤维素转化利用方面发挥核心作用。解淀粉芽孢杆菌是一类具有纤维素酶合成能力的细菌,其合成的纤维素酶具有产酶条件简单,培养周期短,酶活力高的特点,因此具有广泛的应用价值和前景。对近年来产纤维素酶解淀粉芽孢杆菌的筛选与发酵工艺及其特性进行综述,以期为纤维素酶的生产和应用提供理论帮助。
Cellulose is a rich natural biopolymer on the earth. It is an important renewable organic resource and is especially abundant in crop straw.Lignocellulose consists of a mixture of cellulose,hemicellulose and lignin.But the complexity of lignocellulosic structure limits the degradation of large amounts of straw in nature.Cellulase is a general term for a class of enzymes that degrade cellulose into glucose. It mainly acts on cellulose and its derivatives and plays a central role in the conversion and utilization of lignocellulose.Bacillus amyloliquefaciens is a kind of bacteria with cellulase synthesis ability. Its synthesized cellulase has the characteristics of simple enzyme production conditions,short culture period and high enzyme activity. So it has wide application value and prospect.In this paper,the screening,fermentation process and characteristics of cellulase-producing Bacillus amyloliquefaciens were reviewed in order to provide theoretical help for the production and application of cellulase.
Cellulose is a rich natural biopolymer on the earth. It is an important renewable organic resource and is especially abundant in crop straw.Lignocellulose consists of a mixture of cellulose,hemicellulose and lignin.But the complexity of lignocellulosic structure limits the degradation of large amounts of straw in nature.Cellulase is a general term for a class of enzymes that degrade cellulose into glucose. It mainly acts on cellulose and its derivatives and plays a central role in the conversion and utilization of lignocellulose.Bacillus amyloliquefaciens is a kind of bacteria with cellulase synthesis ability. Its synthesized cellulase has the characteristics of simple enzyme production conditions,short culture period and high enzyme activity. So it has wide application value and prospect.In this paper,the screening,fermentation process and characteristics of cellulase-producing Bacillus amyloliquefaciens were reviewed in order to provide theoretical help for the production and application of cellulase.
引文
[1] 张斯童,兰雪,李哲,等.微生物降解玉米秸秆的研究进展[J].吉林农业大学学报,2016,38(5):517-522.
[2] 王华,何玉凤,何文娟,等.纤维素的改性及在废水处理中的应用研究进展[J].水处理技术,2012,38(5):1-6.
[3] 武林芝.香菇纤维素酶基因exg1的克隆及其表达[D].太原:山西大学,2011.
[4] 洪鹏,安国栋,胡梅英,等.解淀粉芽孢杆菌HF-01发酵条件优化[J].中国生物防治学报,2013,29(4):569-578.
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[6] 高颀.纤维素酶在黄酒酿造中的应用[J].中国食品,2011(5):59-61.
[7] 吴斌,胡肄珍.产纤维素酶放线菌的研究进展[J].中国酿造,2008,27(1):5-8.
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[11] BNASAL N,TEWARI R,SONI R,et al.Production of cellulases from Aapergillus niger NS-2 in solid state fementation on agricultural and kitchen waste residues[J].Waste Manag,2012,32(7):1341-1346.
[12] SáNCHEZ C.Lignocellulosic residues:Biodegradation and bioconversion by fungi[J].Biotechnol Adv,2009,27(2):185-194.
[13] 戚耀之.基于C-端氨基酸突变改造提高内切型纤维素酶活性和稳定性的研究[D].南京:南京林业大学,2013.
[14] 唐开宇,张全,佟明友.β-葡萄糖苷酶发酵技术的进展[J].纤维素科学与技术,2009,17(3):65-70.
[15] 孙建义,许梓荣.芽孢杆菌的分离、鉴定及应用研究[J].浙江农业学报,1999,11(1):47-50.
[16] 罗明坤.解淀粉芽孢杆菌在对虾养殖中的应用[J].科学养鱼,2015,31(11):92.
[17] KUMAR P N,SWAPNA T H,KHAN M Y,et al.Statistical optimization of antifungal iturin A production from Bacillus amyloliquefaciens RHNK22 using agro-industrial wastes[J].Saudi journal of biological sciences,2017,24(7):1722-1740.
[18] GUPTA R,VAKHLU J,AGARWAL A,et al.Draft genome sequence of plant growth-promoting Bacillus amyloliquefaciens strain W2 associated with Crocus sativus (Saffron)[J].Genome announcements,2014,2(5):1.
[19] 彭研.拮抗解淀粉芽孢杆菌的筛选鉴定及其产胞外抗菌物质的初步研究[D].曲阜:曲阜师范大学,2013.
[20] 李蘅香,王凤学,钟超,等.1株解淀粉芽孢杆菌CEL-1发酵产纤维素酶的条件优化[J].江苏农业科学,2015,43(12):379-382.
[21] 刘宇,李阳,尹珺伊,等.一株产纤维素酶的解淀粉芽孢杆菌的分离鉴定及其酶促反应适宜条件初步研究[J].家畜生态学报,2017,38(4):58-62.
[22] 林晓琼,孙旸,陈光,等.常压室温等离子体快速诱变筛选高产纤维素酶生产菌株[J] .吉林农业大学学报,2016,38(5):543-547.
[23] 姜军坡,朱宝成,王世英.以滤纸酶活力为指标优化解淀粉芽孢杆菌Tu-115菌株产纤维素酶液体发酵条件[J].饲料工业,2014,35(20):43-47.
[24] 李红亚,李文,李术娜,等.解淀粉芽孢杆菌复合菌剂对玉米秸秆的降解作用及表征[J].草业学报,2017,26(6):153-167.
[25] 邓小燕.抗寒基因表达载体构建及遗传转化研究[D].重庆:西南农业大学,2003.
[26] CHEN S,PU H M,ZHANG J F,et al.Transformation of Brassica napus L.with an ihpRNA vector targeting the A-12 desaturase gene to achieve high oleic acid germplasm[C]//第三届植物分子育种国际会议论文集.北京:中国农学会,2010.
[27] 陈小玲,张穗生,黄俊,等.里氏木霉纤维二糖水解酶基因cbh1的分子改造[J].南方农业学报,2014,45(10):1739-1743.
[28] 熊小龙.应用于宏基因组纤维素酶基因钓取的新PCR方法的构建[D].杭州:杭州师范大学,2012.
[29] 陈玉娟,沈微,陈献忠,等.解淀粉芽孢杆菌β-1,3-1,4-葡聚糖酶的高效表达[J].生物技术,2011,21(2):22-26.
[30] 苗会.耐碱内切葡聚糖酶的定向进化[D].长春:吉林农业大学,2017.
[31] WONG J H,HAO J,CAOZ,et al.An antifungal protein form Bacillus amyloliquefaciens[J].J Appl Microbiol,2008,105(6):1888-1898.
[32] 邓建良,刘红彦,刘玉霞,等.解淀粉芽孢杆菌 YN-1抑制植物病原真菌活性物质鉴定[J].植物病理学报,2010,40(2):202-209.
[33] 胡忠亮,郑催云,田兴一,等.解淀粉芽孢杆菌在环境保护和农业生产中的应用[J].农药,2016(4):241-245.
[34] 孙玲红.鹅盲肠产纤维素酶细菌的分离鉴定及酶学性质研究[D].合肥:安徽农业大学,2016.
[35] 胡聪,李仲玄,斯大勇,等.解淀粉芽孢杆菌在畜禽养殖中的应用[J].饲料工业,2018(6):56-60.
[36] 高灵芳.木质纤维素降解菌产糖特性及作用机制[D].哈尔滨:哈尔滨工业大学,2013.
[2] 王华,何玉凤,何文娟,等.纤维素的改性及在废水处理中的应用研究进展[J].水处理技术,2012,38(5):1-6.
[3] 武林芝.香菇纤维素酶基因exg1的克隆及其表达[D].太原:山西大学,2011.
[4] 洪鹏,安国栋,胡梅英,等.解淀粉芽孢杆菌HF-01发酵条件优化[J].中国生物防治学报,2013,29(4):569-578.
[5] 梁艳玲.产纤维素酶细菌的分离、鉴定及地类芽孢杆菌ME27-1产酶条件优化[D].南宁:广西大学,2014.
[6] 高颀.纤维素酶在黄酒酿造中的应用[J].中国食品,2011(5):59-61.
[7] 吴斌,胡肄珍.产纤维素酶放线菌的研究进展[J].中国酿造,2008,27(1):5-8.
[8] 曹平,李敏,周非帆,等.瘤胃纤维降解细菌的发酵和纤维降解特性研究[J].家畜生态学报,2015,36(9):36-39.
[9] 钱文佳.南极细菌产低温纤维素酶的研究[D].哈尔滨:哈尔滨工业大学,2009.
[10] TAKASHIMA S,IIKURA H,NAKAMURA A,et al.Isolation of the gene and characterization of the enzymatic properties of a major exoglucanase of humicola grisea without a cellulose-binding domain[J].Biochemistry,1998,124(4):717-725.
[11] BNASAL N,TEWARI R,SONI R,et al.Production of cellulases from Aapergillus niger NS-2 in solid state fementation on agricultural and kitchen waste residues[J].Waste Manag,2012,32(7):1341-1346.
[12] SáNCHEZ C.Lignocellulosic residues:Biodegradation and bioconversion by fungi[J].Biotechnol Adv,2009,27(2):185-194.
[13] 戚耀之.基于C-端氨基酸突变改造提高内切型纤维素酶活性和稳定性的研究[D].南京:南京林业大学,2013.
[14] 唐开宇,张全,佟明友.β-葡萄糖苷酶发酵技术的进展[J].纤维素科学与技术,2009,17(3):65-70.
[15] 孙建义,许梓荣.芽孢杆菌的分离、鉴定及应用研究[J].浙江农业学报,1999,11(1):47-50.
[16] 罗明坤.解淀粉芽孢杆菌在对虾养殖中的应用[J].科学养鱼,2015,31(11):92.
[17] KUMAR P N,SWAPNA T H,KHAN M Y,et al.Statistical optimization of antifungal iturin A production from Bacillus amyloliquefaciens RHNK22 using agro-industrial wastes[J].Saudi journal of biological sciences,2017,24(7):1722-1740.
[18] GUPTA R,VAKHLU J,AGARWAL A,et al.Draft genome sequence of plant growth-promoting Bacillus amyloliquefaciens strain W2 associated with Crocus sativus (Saffron)[J].Genome announcements,2014,2(5):1.
[19] 彭研.拮抗解淀粉芽孢杆菌的筛选鉴定及其产胞外抗菌物质的初步研究[D].曲阜:曲阜师范大学,2013.
[20] 李蘅香,王凤学,钟超,等.1株解淀粉芽孢杆菌CEL-1发酵产纤维素酶的条件优化[J].江苏农业科学,2015,43(12):379-382.
[21] 刘宇,李阳,尹珺伊,等.一株产纤维素酶的解淀粉芽孢杆菌的分离鉴定及其酶促反应适宜条件初步研究[J].家畜生态学报,2017,38(4):58-62.
[22] 林晓琼,孙旸,陈光,等.常压室温等离子体快速诱变筛选高产纤维素酶生产菌株[J] .吉林农业大学学报,2016,38(5):543-547.
[23] 姜军坡,朱宝成,王世英.以滤纸酶活力为指标优化解淀粉芽孢杆菌Tu-115菌株产纤维素酶液体发酵条件[J].饲料工业,2014,35(20):43-47.
[24] 李红亚,李文,李术娜,等.解淀粉芽孢杆菌复合菌剂对玉米秸秆的降解作用及表征[J].草业学报,2017,26(6):153-167.
[25] 邓小燕.抗寒基因表达载体构建及遗传转化研究[D].重庆:西南农业大学,2003.
[26] CHEN S,PU H M,ZHANG J F,et al.Transformation of Brassica napus L.with an ihpRNA vector targeting the A-12 desaturase gene to achieve high oleic acid germplasm[C]//第三届植物分子育种国际会议论文集.北京:中国农学会,2010.
[27] 陈小玲,张穗生,黄俊,等.里氏木霉纤维二糖水解酶基因cbh1的分子改造[J].南方农业学报,2014,45(10):1739-1743.
[28] 熊小龙.应用于宏基因组纤维素酶基因钓取的新PCR方法的构建[D].杭州:杭州师范大学,2012.
[29] 陈玉娟,沈微,陈献忠,等.解淀粉芽孢杆菌β-1,3-1,4-葡聚糖酶的高效表达[J].生物技术,2011,21(2):22-26.
[30] 苗会.耐碱内切葡聚糖酶的定向进化[D].长春:吉林农业大学,2017.
[31] WONG J H,HAO J,CAOZ,et al.An antifungal protein form Bacillus amyloliquefaciens[J].J Appl Microbiol,2008,105(6):1888-1898.
[32] 邓建良,刘红彦,刘玉霞,等.解淀粉芽孢杆菌 YN-1抑制植物病原真菌活性物质鉴定[J].植物病理学报,2010,40(2):202-209.
[33] 胡忠亮,郑催云,田兴一,等.解淀粉芽孢杆菌在环境保护和农业生产中的应用[J].农药,2016(4):241-245.
[34] 孙玲红.鹅盲肠产纤维素酶细菌的分离鉴定及酶学性质研究[D].合肥:安徽农业大学,2016.
[35] 胡聪,李仲玄,斯大勇,等.解淀粉芽孢杆菌在畜禽养殖中的应用[J].饲料工业,2018(6):56-60.
[36] 高灵芳.木质纤维素降解菌产糖特性及作用机制[D].哈尔滨:哈尔滨工业大学,2013.