丙氨瑞林注射剂对子宫内膜癌前病变患者脆性组氨酸三联体基因表达的影响
|
摘要
目的观察丙氨瑞林对子宫内膜癌前病变患者脆性组氨酸三联体(FHIT)基因表达的影响。方法 40例子宫内膜癌前病变患者,给予丙氨瑞林150μg,皮下注射,每月1次,4个月为1个疗程,连续治疗2个疗程。分别于治疗前及治疗2个疗程后采集患者子宫内膜组织,用反转录酶-聚合酶链锁反应(RTPCR)及免疫组化法检测子宫内膜组织FHIT基因及蛋白表达水平;分别于治疗前及治疗1,2个疗程后,检查患者子宫膜厚度变化。结果治疗前、治疗2个疗程后FHIT基因相对表达量分别为1. 03±0. 25,2. 68±0. 36; FHIT蛋白免疫组化评分分别为(2. 35±0. 54),(6. 54±1. 23)分,差异均有统计学意义(均P <0. 05);治疗前、治疗1,2个疗程后子宫内膜厚度分别为(9. 84±2. 33),(5. 12±1. 62),(3. 98±0. 69) mm,差异均有统计学意义(均P <0. 05)。结论丙氨瑞林可有效增加子宫内膜癌前病变患者子宫内膜组织中FHIT基因及蛋白表达水平,进而改善患者临床症状。
Objective To investigate the effect of alanine on the expression of fragile histidine triad( FHIT) gene in patients with endometrial precancerous lesions. Methods Forty patients with endometrial precancerous lesions were given alanethrin 150 μg,im,once a month,the patients were given 2 courses treatment( 1 course = 4 months). The patients' endometrial tissues were collected before and after treatment,and the expression of FHIT gene and protein in endometrium were detected by reverse transcriptase-polymerase chain reaction( RT-PCR) and immunohistochemistry,respectively. The patient's uterine membrane thickness changes were detected. Results The relative expression of FHIT gene before treatment and after 2 courses of treatment were 1. 03 ± 0. 25,2. 68 ± 0. 36,respectively; FHIT protein immunohistochemical scores were 2. 35 ± 0. 54,6. 54 ± 1. 23,the difference was statistically significant( P < 0. 01); The endometrial thickness after 1 and 2 courses of treatment were( 5. 12 ± 1. 62),( 3. 98 ± 0. 69) mm,significance was found compared with that before treatment [( 9. 84 ± 2. 33) mm]( P < 0. 01). Conclusion Alanine can effectively increase the expression of FHIT gene and protein in endometrial tissue of patients with endometrial precancerous lesions,and thus promote the improvement of clinical symptoms.
Objective To investigate the effect of alanine on the expression of fragile histidine triad( FHIT) gene in patients with endometrial precancerous lesions. Methods Forty patients with endometrial precancerous lesions were given alanethrin 150 μg,im,once a month,the patients were given 2 courses treatment( 1 course = 4 months). The patients' endometrial tissues were collected before and after treatment,and the expression of FHIT gene and protein in endometrium were detected by reverse transcriptase-polymerase chain reaction( RT-PCR) and immunohistochemistry,respectively. The patient's uterine membrane thickness changes were detected. Results The relative expression of FHIT gene before treatment and after 2 courses of treatment were 1. 03 ± 0. 25,2. 68 ± 0. 36,respectively; FHIT protein immunohistochemical scores were 2. 35 ± 0. 54,6. 54 ± 1. 23,the difference was statistically significant( P < 0. 01); The endometrial thickness after 1 and 2 courses of treatment were( 5. 12 ± 1. 62),( 3. 98 ± 0. 69) mm,significance was found compared with that before treatment [( 9. 84 ± 2. 33) mm]( P < 0. 01). Conclusion Alanine can effectively increase the expression of FHIT gene and protein in endometrial tissue of patients with endometrial precancerous lesions,and thus promote the improvement of clinical symptoms.
引文
[1]AHMAD K,KIM Y H,DEPPE G,et al. Radiation therapy in stageⅡcarcinoma of the endometrium[J]. Cancer,2015,63(5):854-858.
[2]刘韵,兰艳丽.丙氨瑞林对子宫内膜癌Chk1/2和Plk1蛋白表达的影响研究[J].中国生化药物杂志,2015,35(1):103-105.
[3]DEMOKAN S,AKALIN K,OZKOKLESEN C,et al. Investigation of the promoter methylation status of the FHIT(fragile histidine triad)gene in larynx cancer[J]. Can Res,2016,76(14 Supplement):S4443.
[4]DMITRY Z,BOERSMA A W M,YING C,et al. MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation[J]. Int J Leg Med,2010,124(3):217-226.
[5]PHILLIPS T,SIMMONS P,INZUNZA H D,et al. Development of an automated PD-L1 immunohistochemistry(IHC)assay for nonsmall cell lung cancer[J]. APPL Immunohisto M M,2015,23(8):541-541.
[6]HIGHAM JM,LECTURER PMSOS,SHAW RW. Assessment of menstrual blood loss using a pictorial chart[J]. Int J Obstet Gynaecol,1990,97(8):734-734.
[7]MOXON R,WHITESIDE H,ENGLAND G C. Prevalence of ultrasound-determined cystic endometrial hyperplasia and the relationship with age in dogs[J]. Theriogenol,2016,86(4):976-980.
[8]刘学智,南晶,李娟,等. FHIT基因甲基化及蛋白表达与HPV16感染在宫颈癌变中的交互效应[J].中华流行病学杂志,2016,37(6):858-862.
[9]DIAZ R J,GUDUK M,ROMAGNUOLO R,et al. High-resolution whole-genome analysis of skull base chordomas implicates fhit loss in chordoma pathogenesis[J]. Neoplasia,2012,14(9):788-798.
[2]刘韵,兰艳丽.丙氨瑞林对子宫内膜癌Chk1/2和Plk1蛋白表达的影响研究[J].中国生化药物杂志,2015,35(1):103-105.
[3]DEMOKAN S,AKALIN K,OZKOKLESEN C,et al. Investigation of the promoter methylation status of the FHIT(fragile histidine triad)gene in larynx cancer[J]. Can Res,2016,76(14 Supplement):S4443.
[4]DMITRY Z,BOERSMA A W M,YING C,et al. MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation[J]. Int J Leg Med,2010,124(3):217-226.
[5]PHILLIPS T,SIMMONS P,INZUNZA H D,et al. Development of an automated PD-L1 immunohistochemistry(IHC)assay for nonsmall cell lung cancer[J]. APPL Immunohisto M M,2015,23(8):541-541.
[6]HIGHAM JM,LECTURER PMSOS,SHAW RW. Assessment of menstrual blood loss using a pictorial chart[J]. Int J Obstet Gynaecol,1990,97(8):734-734.
[7]MOXON R,WHITESIDE H,ENGLAND G C. Prevalence of ultrasound-determined cystic endometrial hyperplasia and the relationship with age in dogs[J]. Theriogenol,2016,86(4):976-980.
[8]刘学智,南晶,李娟,等. FHIT基因甲基化及蛋白表达与HPV16感染在宫颈癌变中的交互效应[J].中华流行病学杂志,2016,37(6):858-862.
[9]DIAZ R J,GUDUK M,ROMAGNUOLO R,et al. High-resolution whole-genome analysis of skull base chordomas implicates fhit loss in chordoma pathogenesis[J]. Neoplasia,2012,14(9):788-798.