碳酸盐与磷酸盐对Biohydrogenbacterium R3 sp.nov.产氢发酵效能的影响
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摘要
采用NH4HCO3、NaHCO3、Na2CO3、K2HPO4、KH2HPO4、NaH2PO4和Na2HPO4作为碳酸盐和磷酸盐缓冲溶液源,研究其对Biohydrogenbacterium R3 sp.nov.产氢发酵效能的影响.研究发现,碳酸盐对Biohydrogenbacterium R3 sp.nov.反应体系中的pH值都具有较好的缓冲作用,而磷酸盐的添加对其产氢效能的促进作用较明显.反应器内添加了Na2HPO4与K2HPO4的Biohydrogenbacterium R3 sp.nov.的氢气产量与氢气含量达到最高值,分别为1978.56 mL、44.1%与2160.9 mL、45.8%,此时反应器内的pH值分别为3.28和3.41,细胞浓度分别为1.03 g·L-1和1.21 g·L-1,添加了Na2HPO4的Biohydrogenbacterium R3 sp.nov.的乙醇和乙酸产量分别为4841.49 mg·L-1和2064.94 mg·L-1;而添加了Na2CO3的氢气产量、氢气含量、细胞浓度与反应器内pH值分别为1064.42 mL、35.96%、1.23 g·L-1与3.81,此时乙醇、乙酸的产量分别为3862.21 mg·L-1和1930.86mg·L-1.
This study investigated the effects of phosphates and carbonates(NH4HCO3,NaHCO3,Na2CO3,K2HPO4,KH2PO4,NaH2PO4 and Na2HPO4) on the hydrogen production efficiency and cell growth to Biohydrogenbacterium R3 sp.nov.It was indicated that the carbonates could buffer the pH in reactor better and the phosphates could promote the hydrogen production obviously.When Na2HPO4 and K2HPO4 were added into the reactor,the hydrogen production and content reached the maximum of 1978.56 mL,44.1% and 2160.9 mL,45.8%,respectively.The pH and cell weight achieved 3.28,1.03 g · L-1 and 3.41,1.21 g · L-1,respectively.When Na2HPO4 was added into the reactor,the ethanol and acetic acid were 4841.49 mg · L-1 and 2064.94 mg · L-1,respectively,while when Na2CO3 was added into the reactor,the hydrogen production,hydrogen content,cell weight,pH,ethanol and acetic acid were 1064.42 mL,35.96%,1.23 g · L-1,3.81,3862.21 mg · L-1 and 1930.86 mg · L-1,respectively.
This study investigated the effects of phosphates and carbonates(NH4HCO3,NaHCO3,Na2CO3,K2HPO4,KH2PO4,NaH2PO4 and Na2HPO4) on the hydrogen production efficiency and cell growth to Biohydrogenbacterium R3 sp.nov.It was indicated that the carbonates could buffer the pH in reactor better and the phosphates could promote the hydrogen production obviously.When Na2HPO4 and K2HPO4 were added into the reactor,the hydrogen production and content reached the maximum of 1978.56 mL,44.1% and 2160.9 mL,45.8%,respectively.The pH and cell weight achieved 3.28,1.03 g · L-1 and 3.41,1.21 g · L-1,respectively.When Na2HPO4 was added into the reactor,the ethanol and acetic acid were 4841.49 mg · L-1 and 2064.94 mg · L-1,respectively,while when Na2CO3 was added into the reactor,the hydrogen production,hydrogen content,cell weight,pH,ethanol and acetic acid were 1064.42 mL,35.96%,1.23 g · L-1,3.81,3862.21 mg · L-1 and 1930.86 mg · L-1,respectively.
引文
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Zhu H G,Parker W,Basnar R,et al.2009.Buffer requirements forenhanced hydrogen production in acidogenic digestion of food wastes[J].Bioresource Technology,100(21):5097-5102
洪天求,郝小龙,俞汉青.2004.Na+离子浓度对厌氧发酵产氢气影响的实验研究[J].水处理技术,30(5):270-275
李永峰,任南琪,杨传平,等.2005.一株高效产氢产酸细菌的鉴定与产氢特性[J].中国环境科学,25(2):210-213
Lin C Y,Lay C H.2004.Effects of carbonate and phosphateconcentrations on hydrogen production using anaerobic sewage sludgemicroflora[J].International Journal of Hydrogen Energy,29(3):275-281
林明,任南琪,马汐平,等.2003.产氢发酵细菌培养基的选择和改进[J].哈尔滨工业大学学报,35(4):398-402
Liu Q,Zhang X L,Zhao J,et al.2012.Effect of carbonate on anaerobicacidogenesis and fermentative hydrogen production from glucoseusing leachate as supplementary culture under alkaline conditions[J].Bioresource Technology,113:37-43
Ren N Q,Wang D Y,Yang C P,et al.2010.Selection and isolation ofhydrogen-producing fermentative bacteria with high yield and rateand its bioaugmentation process[J].International Journal ofHydrogen Energy,35:2877-2882
Sontaya K,Pensri P,Alissara R.2011.Optimization of fermentativehydrogen production from hydrolysate of microwave assisted sulfuricacid pretreated oil palm trunk by hot spring enriched culture[J].International Journal of Hydrogen Energy,21(36):14204-14216
国家环保总局.2006.水和废水检测分析方法(第4版)[M].北京:中国环境科学出版社.102-210
王家卓,王建龙.2008.缓冲体系对厌氧发酵生物产氢的影响[J].环境科学学报,28(6):1136-1140
Zhu H G,Parker W,Basnar R,et al.2009.Buffer requirements forenhanced hydrogen production in acidogenic digestion of food wastes[J].Bioresource Technology,100(21):5097-5102