鲁斯可皂苷元制备方法研究
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摘要
目的探寻从麦冬中分离、纯化鲁斯可皂苷元的方法。方法 80%乙醇提取总皂苷,两相酸水解总皂苷,硅胶柱层析分离,甲醇重结晶,薄层色谱(TLC)法、高效液相色谱(HPLC)法检查纯度。结果鲁斯可皂苷元经TLC检查与对照品一致,无杂质斑点,HPLC法检查纯度达98.58%。结论该试验中建立的制备方法简单、安全、可行性强,成本低,效率高,可用于制备鲁斯可皂苷元。
Objective To investigate the method of separating and purifying ruscogenin from Ophiopogon japonicus. Methods 80%ethanol was used to extract the total saponins,two- phase acid hydrolysis of total saponins,silica gel column chromatography separation,methanol recrystallization; TLC,HPLC methods were used to check the purity. Results Ruscogenin was consistent with the control substance by TLC and no impurity spots. The purity was 98. 58% by HPLC. Conclusion The preparation method is simple,safe,feasible with low cost and high efficiency. The obtained ruscogenin can be used as a reference substance for qualitative and quantitative analysis of traditional Chinese medicine.
Objective To investigate the method of separating and purifying ruscogenin from Ophiopogon japonicus. Methods 80%ethanol was used to extract the total saponins,two- phase acid hydrolysis of total saponins,silica gel column chromatography separation,methanol recrystallization; TLC,HPLC methods were used to check the purity. Results Ruscogenin was consistent with the control substance by TLC and no impurity spots. The purity was 98. 58% by HPLC. Conclusion The preparation method is simple,safe,feasible with low cost and high efficiency. The obtained ruscogenin can be used as a reference substance for qualitative and quantitative analysis of traditional Chinese medicine.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:155.
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[10]汪波,丁德秭.四君子汤合沙参麦冬汤加减配合化疗治疗中晚期非小细胞肺癌临床观察[J].世界中西医结合杂志,2009,4(8):564-566.
[11]周宇辉,詹,唐于平,等.麦门冬合千金苇茎汤抑制A549细胞增殖作用及其机制[J].中国肺癌杂志,2010,13(5):477-482.
[12]苟兴能,张克英,杨兴江,等.川麦冬多糖对恒磁场致小鼠免疫损伤的防护作用[J].四川中医,2009,27(5):18-20.
[13]Xiong SL,Hou DB,Hang N,et al.Preparation and biological activity of saponin from Ophiopogon japonicas[J].Afr J Pharm Pharmacol,2012,6(26):1964-1970.
[14]马海波.麦冬化学成分的研究[D].北京:北京中医药大学,2013.
[2]马艳春,朱丹妮,余伯阳,等.麦冬水提物抗急性心肌缺血活性部位的初步筛选[J].时珍国医国药,2013,24(3):561-563.
[3]Wang S,Zhang Z,Lin X,et al.A polysaccharide,MDG-1,induces S1P1 and b FGF expressionand augments survival andangiogenesis in the ischemic heart[J].Glycobiology,2010,20(4):473-484.
[4]Tao J,Wang H,Zhou H,et al.The saponin monomer of dwarf lilyturf tuber,DT-13,reduces L-type calcium currents during hypoxia in adult rat ventricular myocytes[J].Life Sci,2005,77(24):3021-3030.
[5]许洁.麦冬多糖MDG-1口服降糖及作用机制研究[D].上海:上海中医药大学,2011.
[6]毛讯.麦冬天冬提取物对糖尿病小鼠空腹血糖及胸腺、脾脏指数的影响[J].中国老年学杂志,2010,30(13):1861-1862.
[7]田友清,寇俊萍,李林洲,等.短葶山麦冬水提物及其主要有效部位和成的抗炎活性[J].中国天然药物,2011,9(3):222-226.
[8]Hung TM,Thu CV,Dat NT,et,al.Homoisoflavonoid derivatives from the roots of Ophiopogon japonicus and their in vitro anti-inflammolation activity[J].Bioorg Med Chem Lett,2010,20(8):2412-2416.
[9]Sun L,Lin S.The saponin monomer of dwarf lilyturf tuber,DT-13,reduces human breast cancer cell adhesion and migration during hypoxia via regulation of tissue factor[J].Biol Pharm Bull,2010,33(7):1192-1198.
[10]汪波,丁德秭.四君子汤合沙参麦冬汤加减配合化疗治疗中晚期非小细胞肺癌临床观察[J].世界中西医结合杂志,2009,4(8):564-566.
[11]周宇辉,詹,唐于平,等.麦门冬合千金苇茎汤抑制A549细胞增殖作用及其机制[J].中国肺癌杂志,2010,13(5):477-482.
[12]苟兴能,张克英,杨兴江,等.川麦冬多糖对恒磁场致小鼠免疫损伤的防护作用[J].四川中医,2009,27(5):18-20.
[13]Xiong SL,Hou DB,Hang N,et al.Preparation and biological activity of saponin from Ophiopogon japonicas[J].Afr J Pharm Pharmacol,2012,6(26):1964-1970.
[14]马海波.麦冬化学成分的研究[D].北京:北京中医药大学,2013.