针刺百会和风池穴对神经性皮炎模型大鼠P物质影响实验研究
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摘要
目的:研究针刺百会、风池穴和中药口服结合对神经性皮炎模型大鼠P物质的影响,探讨和分析针刺百会、风池穴治疗神经性皮炎机制,为临床治疗该病提供有效的治疗方法。方法:采用慢性轻度不可预见性的应激方法与局部皮肤摩擦刺激相结合制备神经性皮炎大鼠模型,在造模成功的基础上,给予针刺百会、风池穴及中药口服,ELISA法检测皮损组织中P物质。结果:模型组大鼠皮损组织中P物质水平明显升高,差异均具有统计学意义(P<0.01);各治疗组大鼠皮损组织中P物质水平均有不同程度降低,与模型组相比较,差异具有统计学意义(P<0.01)。同时3种治疗方法进行比较,大鼠皮损组织中P物质水平降低以针药组最为明显,针刺组次之。结论:针刺百会、风池穴和中药口服联合应用治疗神经性皮炎的机制可能为降低瘙痒介质P物质的水平,从而起到缓解瘙痒的作用,对神经性皮炎起到治疗作用,为临床治疗神经性皮炎提供了有效的治疗方法。
Objective:To study the effect of acupuncture at Baihui,Fengchi and oral liquid of traditional Chinese medicine on substance P in rats model with neurodermatitis,to explore and analyze the mechanism of acupuncture on Baihui and Fengchi acupoints in the treatment of neurodermatitis,to provide effective clinical treatment for neurodermatitis. Methods:A rat model of neurodermatitis was established by combining chronic mild unpredictability stress with local skin friction stimulation on the basis of successful modeling,acupuncturing at Baihui,Fengchi and taking oral liquid of traditional Chinese medicine,then,detected the substance P in skin lesions using ELISA method. At the end. Results:The level of substance P in the lesions of rats model was significantly higher than that in the other groups,the difference has statistical significance.(P<0.01). The level of substance P in the lesions of rats in each treatment group decreased to different degrees,and the difference was statistically significant compared with the model group(P<0.01). Three treatments were compared at the same time,the decrease of substance P level in rat skin lesions was most obvious in the group of acupuncture combined with oral liquid of traditional Chinese medicine,followed by the acupuncture group. Conclusion:It is suggested that the mechanism of acupuncture at Baihui,Fengchi and oral liquid of traditional Chinese medicine in the treatment of neurodermatitis maybe reduce the level of substance P in itching mediator,thereby it alleviates itching,treats neurodermatitis and provides an effective treatment method for clinical treatment of neurodermatitis.
Objective:To study the effect of acupuncture at Baihui,Fengchi and oral liquid of traditional Chinese medicine on substance P in rats model with neurodermatitis,to explore and analyze the mechanism of acupuncture on Baihui and Fengchi acupoints in the treatment of neurodermatitis,to provide effective clinical treatment for neurodermatitis. Methods:A rat model of neurodermatitis was established by combining chronic mild unpredictability stress with local skin friction stimulation on the basis of successful modeling,acupuncturing at Baihui,Fengchi and taking oral liquid of traditional Chinese medicine,then,detected the substance P in skin lesions using ELISA method. At the end. Results:The level of substance P in the lesions of rats model was significantly higher than that in the other groups,the difference has statistical significance.(P<0.01). The level of substance P in the lesions of rats in each treatment group decreased to different degrees,and the difference was statistically significant compared with the model group(P<0.01). Three treatments were compared at the same time,the decrease of substance P level in rat skin lesions was most obvious in the group of acupuncture combined with oral liquid of traditional Chinese medicine,followed by the acupuncture group. Conclusion:It is suggested that the mechanism of acupuncture at Baihui,Fengchi and oral liquid of traditional Chinese medicine in the treatment of neurodermatitis maybe reduce the level of substance P in itching mediator,thereby it alleviates itching,treats neurodermatitis and provides an effective treatment method for clinical treatment of neurodermatitis.
引文
[1]张学军.皮肤性病学[M].北京:人民卫生出版社,2013:136.
[2]马天明,程为平,李然.针刺百会和风池穴对神经性皮炎模型大鼠中枢神经递质的影响[J].辽宁中医药大学学报,2017,19(7):23-25.
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[5]徐叔云.药理实验方法学[M].北京:人民卫生出版社,1994:642-643.
[6]丁媛,普雄明.瘙痒的发生机制、相关疾病和治疗[J].中国麻风皮肤病杂志,2006,22(6):492-495.
[7]桑洁玉,郑瑞.皮肤瘙痒神经传导介质的研究进展[J].中国医学创新,2015,12(2):151.
[2]马天明,程为平,李然.针刺百会和风池穴对神经性皮炎模型大鼠中枢神经递质的影响[J].辽宁中医药大学学报,2017,19(7):23-25.
[3]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003.
[4]Vecsei L.Alling C,Heilig M,et al. Effects of cysteamine and pantethine on open-field behavior,hypothalamic catecholamine concentrations,and somatostatin-induced barrel rotation in rats[J].Pharmacol Biochem Behav,1989,32(3):629-635.
[5]徐叔云.药理实验方法学[M].北京:人民卫生出版社,1994:642-643.
[6]丁媛,普雄明.瘙痒的发生机制、相关疾病和治疗[J].中国麻风皮肤病杂志,2006,22(6):492-495.
[7]桑洁玉,郑瑞.皮肤瘙痒神经传导介质的研究进展[J].中国医学创新,2015,12(2):151.