心脑舒通胶囊对氧糖剥夺/复氧星形胶质细胞神经营养因子分泌的影响及对神经元的保护作用
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摘要
[目的]探讨心脑舒通胶囊对氧糖剥夺/复氧(OGD/R)处理的星形胶质细胞(C8-D1A)神经生长因子分泌的影响,以及研究心脑舒通药物处理的OGD/R胶质细胞条件培养液对神经元细胞(Neuro-2A)的保护作用。[方法] CCK-8检测心脑舒通对正常培养和OGD/R胶质细胞活力的影响;酶联免疫吸附(ELISA)法检测心脑舒通对OGD/R胶质细胞的脑源性神经营养因子(BDNF)、胶质源性神经营养因子(GDNF)、睫状神经营养因子(CNTF)释放的影响;CCK-8检测心脑舒通处理的胶质细胞条件培养液对OGD/R神经元细胞活力的影响。[结果] 1)心脑舒通对正常培养的胶质细胞活力无显著影响,但可以提高OGD/R后胶质细胞的增殖活力。2)0.1μg/mL心脑舒通可以显著提高OGD/R胶质细胞BDNF的释放。3)0.1μg/mL心脑舒通药物处理的胶质细胞条件培养液可提高OGD/R神经元的存活能力。[结论]心脑舒通对OGD/R损伤后的胶质细胞有一定保护作用,其处理后的胶质细胞条件培养液对神经元有一定的保护作用。
[Objective] To explore the effect of Xinnao Shutong Capsules on the secretion of nerve growth factor in astrocytes cell induced by oxygen-glucose deprivation/reoxygenation(OGD/R). The protective effects on OGD/R-induced neurons cell variability treated by astrocytes conditioned medium were also explored. [Methods] The effect of Xinnao Shutong Capsules on both normal culture and OGD/R-induced C8-D1 A cell viability were detected by CCK-8. ELISA was adopted to measure the secretion of BDNF, GDNF and CNTF in OGD/R-induced C8-D1 A cell which treatment with Xinnao Shutong Capsules. [Results] 1) Xinnao Shutong Capsules had no significant effect on the viability of normal cultured C8-D1 A cell, but could increase the proliferation of OGD/R-induced C8-D1 A cell.2)Xinnao Shutong Capsules(0.1 μg/mL)could significantly promote the secretion of BDNF on C8-D1 A cell after OGD/R. 3)C8-D1 A cell treated with Xinnao Shutong Capsules(0.1 μg/mL)conditioned medium had protective effect on neurons. [Conclusion] Xinnao Shutong Capsules had protective effect on OGD/R-induced C8-D1 A cell, and its treated C8-D1 A conditioned medium played protective role on OGD/R-induced neurons.
[Objective] To explore the effect of Xinnao Shutong Capsules on the secretion of nerve growth factor in astrocytes cell induced by oxygen-glucose deprivation/reoxygenation(OGD/R). The protective effects on OGD/R-induced neurons cell variability treated by astrocytes conditioned medium were also explored. [Methods] The effect of Xinnao Shutong Capsules on both normal culture and OGD/R-induced C8-D1 A cell viability were detected by CCK-8. ELISA was adopted to measure the secretion of BDNF, GDNF and CNTF in OGD/R-induced C8-D1 A cell which treatment with Xinnao Shutong Capsules. [Results] 1) Xinnao Shutong Capsules had no significant effect on the viability of normal cultured C8-D1 A cell, but could increase the proliferation of OGD/R-induced C8-D1 A cell.2)Xinnao Shutong Capsules(0.1 μg/mL)could significantly promote the secretion of BDNF on C8-D1 A cell after OGD/R. 3)C8-D1 A cell treated with Xinnao Shutong Capsules(0.1 μg/mL)conditioned medium had protective effect on neurons. [Conclusion] Xinnao Shutong Capsules had protective effect on OGD/R-induced C8-D1 A cell, and its treated C8-D1 A conditioned medium played protective role on OGD/R-induced neurons.
引文
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[9]郭大志,冯园,胡慧军,等.高压氧对急性一氧化碳中毒大鼠少突胶质细胞前体细胞ADAM10 m RNA表达的影响[J].中国医药导报,2018,15(5):9-12.
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[11]Sun M,Liu H,Min S,et al.Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons[J].Biomed Rep,2016,4(4):417-420.
[2]Jakovcevski M,Akbarian S,Di Benedetto B.Pharmacologica modulation of astrocytes and the role of cell type-specific histone modifications for the treatment of mood disorders[J].Curr Opin Pharmacol,2016(26):61-66.
[3]Schizas N,Konig N,Andersson B,et al.Neural crest stem cells protect spinal cord neurons from excitotoxic damage and inhibit glia activation by secretion of brain-derived neurotrophic factor[J].Cel Tissue Res,2018,372(3):493-505.
[4]Wei H,Sun T,Tian Y,et al.Ginkgolide B modulates BDNFexpression in acute ischemic stroke[J].J Korean Neurosurg Soc,2017,60(4):391-396.
[5]Li W,Li X,Du Q,et al.Effect of tongluojiunao injection made from sanqi(Radix Notoginseng)and zhizi(Fructus Gardeniae)on brain microvascular endothelial cells and astrocytes in an in vitro ischemic model[J].J Tradit Chin Med,2014,34(6):725-732.
[6]杜宝奎.心脑舒通胶囊治疗脑血栓形成后遗症的临床效果[J].中国实用医药,2015,10(5):29-30.
[7]Ahmad M,Dar NJ,Bhat ZS,et al.Inflammation in ischemic stroke:mechanisms,consequences and possible drug targets[J].CNS Neurol Disord Drug Targets,2014,13(8):1378-1396.
[8]崔鹤鹛.脑卒中后星形胶质细胞的神经保护及神经修复作用及分子机制[D].石家庄:河北医科大学,2017.
[9]郭大志,冯园,胡慧军,等.高压氧对急性一氧化碳中毒大鼠少突胶质细胞前体细胞ADAM10 m RNA表达的影响[J].中国医药导报,2018,15(5):9-12.
[10]Cortes D,Carballo-Molina OA,Castellanos-Montiel MJ,et al.The non-survival effects of glial cell line-derived neurotrophic factor on neural cells[J].Front Mol Neurosci,2017,10:258.
[11]Sun M,Liu H,Min S,et al.Ciliary neurotrophic factor-treated astrocyte-conditioned medium increases the intracellular free calcium concentration in rat cortical neurons[J].Biomed Rep,2016,4(4):417-420.