糖尿病脑病大鼠脑PET/CT显像、胰岛素样生长因子受体及葡萄糖转运蛋白4的表达
摘要
目的观察糖尿病脑病大鼠脑PET/CT显像、胰岛素样生长因子1型受体(IGF-1R)及葡萄糖转运蛋白(GLUT)4的表达。方法采用链脲佐菌素(STZ)诱导大鼠糖尿病模型,Morris水迷宫试验筛选糖尿病脑病大鼠模型,正常对照组,糖尿病组(排除脑病),糖尿病脑病组各随机选取8只,进行脑部PET/CT显像、免疫组化法检测脑组织IGF-1R及GLUT4表达。结果与正常对照组及糖尿病组比较,糖尿病脑病组大鼠标准摄取值(SUV)显著降低(P<0.01);IGF-1R表达显著增高而GLUT4显著下降(P<0.01)。结论糖尿病脑病大鼠脑组织IGF-1R异常高表达,扰乱了脑部糖代谢的相关通路。
引文
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2 Lin YH,Westenbroek C,Tie L,et al.Effects of glucose,insulin,and supernatant from pancreatic beta-cells on brain-pancreas relative protein in rat hippocampus[J].Neurochem Res,2006;31(12):1417-24.
3 Chang HK,Mukes JD,Figgers CL.Ameliorative effects of dietary caloric restriction on oxidative stress and inflammation in the brain of streptozotocin-induced diabetic rats[J].Am J Chin Med,2004;32(4):497-507.
4 Ugochukwu NH,Bady NE.The effect of Gongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats[J].J Biol Sci,2006;370(12):165-73.
5 Elangovan V,Kohen R,Shohami E.Neurological recovery from closed head injury is impaired in diabetic rats[J].J Neurotranma,2000;17(11):1013-27.
6 Dunphy MP,Lewis JS.Radiopharmaceuticals in preclinical and clinical development for monitoring of therapy with PET[J].J Nucl Med,2009;106S-121S.
7 Vallabhajosula S.Molecular imaging:radiopharmaceuticals for PET and SPECT[M].Berlin:Heidelberg,2009:201-8.
8 Thomas T,Thomas G,Mclendon C,et al.β-amyloid-mediated vasoactivity and vascular endothelial damage[J].Nature,1996;380:168-71.
9 Dong XC,Park S,Lin XY.Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth[J].J Clin Invest,2006;1:32-6.
10 Kobayashi M,Nikami H,Morimatsu M,et al.Expression and localization of insulin regulatable glucose transporter(Glut4)in rat brain[J].Neurosci Lett,1966;213:103-6.
11 Messari SE,Leloup C,Quignon M,et al.Immunocytochemical localization of the insulin regulatable glucose transporter(Glut4)in the central nervous system[J].J Comp Neurol,1998;399:492-512.
12 Vannucci SJ,Koehler-Stec EM,Li K,et al.Glut4 glucose transpoter expression in rodent brain effects of diabetes[J].Brain Res,1998;797:1-11.
13 Pardride WM.Brain metabolism:a perspective from the blood-brain barrier[J].Physiol Rev,1983;63:1481-535.
14 Mcewen BS,Reagen LP.Glucose transporter expression in the central nervous system:relationship to synaptic function[J].Eur J Pharmacol,2004;490:13-24.
15 Steen E,Terry BM,Rivera EJ,et al.Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer’s disease is this type diabetes[J].J Alzheimer Dis,2005;7:63-80.