墨旱莲代谢产物的GC-MS分析
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摘要
目的:建立基于GC-MS技术的代谢组学方法,研究不同产地和不同时间采收的墨旱莲代谢产物指纹图谱的差异。方法:优化提取条件及色谱条件,采用主成分分析法对不同产地及不同时间采收的墨旱莲样本进行模式识别。结果:基于优化的提取条件及色谱条件,建立了墨旱莲的GC-MS代谢组学方法,并考察了方法的稳定性,获得了小分子代谢产物的表达谱,发现不同产地及不同时间采收的样本之间代谢产物有显著性差异。结论:优化的GC-MS代谢组学方法可以从代谢水平检测墨旱莲药材,找出差异性,阐述产地及采收时间对代谢物的影响,可为墨旱莲代谢组学的进一步研究提供参考。
Objective:To establish a metabolomics method based on GC-MS technology and study the differences of metabolite fingerprints of Ecliptae Herba harvested from different origins and at different time.Methods:The extraction conditions and chromatographic conditions were optimized,and principal component analysis was used to extract valuable information of the Ecliptae Herba samples harvested from different origins and at different time.Results:Based on the optimized extraction conditions and chromatographic conditions,GC-MS metabolomics method was established,and the stability of the method was investigated.The small molecular metabolite expression profiles were obtained,and significant differences were found among metabolites of Ecliptae Herba harvested from different origins and at different time.Conclusion:The optimized GC-MS metabolomics method could detect the differences of Ecliptae Herba samples from metabolic level,and expound the effect of different origins and harvest time on metabolites.The study could provide reference for further studies on the metabolomics of Ecliptae Herba.
Objective:To establish a metabolomics method based on GC-MS technology and study the differences of metabolite fingerprints of Ecliptae Herba harvested from different origins and at different time.Methods:The extraction conditions and chromatographic conditions were optimized,and principal component analysis was used to extract valuable information of the Ecliptae Herba samples harvested from different origins and at different time.Results:Based on the optimized extraction conditions and chromatographic conditions,GC-MS metabolomics method was established,and the stability of the method was investigated.The small molecular metabolite expression profiles were obtained,and significant differences were found among metabolites of Ecliptae Herba harvested from different origins and at different time.Conclusion:The optimized GC-MS metabolomics method could detect the differences of Ecliptae Herba samples from metabolic level,and expound the effect of different origins and harvest time on metabolites.The study could provide reference for further studies on the metabolomics of Ecliptae Herba.
引文
[1] 国家药典委员会.中华人民共和国药典.2015年版一部[S].北京:中国医药科技出版社,2015:374.State Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China.2015 ed.Volume 1[S].Beijing:Chinese Medical Science Press,2015:374.In Chinese.
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[9] 杨楠,张静,王磊,等.肌醇及其代谢关键酶基因与植物逆境响应机制的研究进展[J].鲁东大学学报(自然科学版),2017,33(4):321-325.YANG Nan,ZHANG Jing,WANG Lei,et al.Research progress in myo-inositol and its metabolic key enzyme genes with plant stress response mechanism[J].J Ludong Univ(Nat Sci Ed),2017,33(4):321-325.In Chinese with English abstract.
[10] 赵瑞雪,朱慧森,程钰宏,等.植物脯氨酸及其合成酶系研究进展[J].草业科学,2008,25(2):90-96.ZHAO Ruixue,ZHU Huisen,CHENG Yuhong,et al.Research progress in proline and its biosynthesis enzymes in plant[J].Pratacultural Sci,2008,25(2):90-96.In Chinese with English abstract.
[2] 方悦,李熙晨,张朝凤.墨旱莲化学成分与药理活性的研究进展[J].海峡药学,2015,27(6):1-3.FANG Yue,LI Xichen,ZHANG Chaofeng.Research advances in chemical constituents and pharmacological effects of Herba Ecliptae[J].Strait Pharm J,2015,27(6):1-3.In Chinese with English abstract.
[3] 淡墨,高先富,谢国祥,等.代谢组学在植物代谢研究中的应用[J].中国中药杂志,2007,32(22):2337-2341.DAN Mo,GAO Xianfu,XIE Guoxiang,et al.Application of metabolomics in research of plant metabolites[J].China J Chin Mater Med,2007,32(22):2337-2341.In Chinese with English abstract.
[4] 段礼新,漆小泉.基于GC-MS的植物代谢组学研究[J].生命科学,2015,27(8):971-977.DUAN Lixin,QI Xiaoquan.GC-MS-based plant metabolomics researches[J].Chin Bull Life Sci,2015,27(8):971-977.In Chinese with English abstract.
[5] 周佳,王霜原,常玉玮,等.基于气相色谱-质谱联用技术的水稻代谢轮廓分析方法的建立[J].色谱,2012,30(10):1037-1042.ZHOU Jia,WANG Shuangyuan,CHANG Yuwei,et al.Development of a gas chromatography-mass spectrometry method for the metabolomic study of rice(Oryza sativa L.) grain[J].Chin J Chromatogr,2012,30(10):1037-1042.In Chinese with English abstract.
[6] M?nchgesang S,Strehmel N,Trutschel D,et al.Plant-to-plant variability in root metabolite profiles of 19 arabidopsis thaliana accessions is substance-class-dependent[J].Int J Mol Sci,2016,17(9):1565-1573.
[7] Fiehn O.Metabolomics-the link between genotypes and phenotypes[J].Plant Mol Biol,2002,48(1-2):155-171.
[8] 杨旭,杨志玲,刘若楠,等.姜黄根茎中姜黄素类成分含量的产地差异及其与环境因子的CCA分析[J].植物资源与环境学报,2011,20(3):16-23.YANG Xu,YANG Zhiling,LIU Ruonan,et al.Location variation of curcuminoids content in Curcuma longa rhizome and CCA analysis with environmental factors[J].J Plant Resour Environ,2011,20(3):16-23.In Chinese with English abstract.
[9] 杨楠,张静,王磊,等.肌醇及其代谢关键酶基因与植物逆境响应机制的研究进展[J].鲁东大学学报(自然科学版),2017,33(4):321-325.YANG Nan,ZHANG Jing,WANG Lei,et al.Research progress in myo-inositol and its metabolic key enzyme genes with plant stress response mechanism[J].J Ludong Univ(Nat Sci Ed),2017,33(4):321-325.In Chinese with English abstract.
[10] 赵瑞雪,朱慧森,程钰宏,等.植物脯氨酸及其合成酶系研究进展[J].草业科学,2008,25(2):90-96.ZHAO Ruixue,ZHU Huisen,CHENG Yuhong,et al.Research progress in proline and its biosynthesis enzymes in plant[J].Pratacultural Sci,2008,25(2):90-96.In Chinese with English abstract.