增强玉米幼苗抗干旱能力内生真菌的筛选
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摘要
室内盆栽条件下,将从玉米及小麦组织中分离的20种内生真菌接种玉米幼苗,采用8%PEG-6000模拟干旱胁迫,比较正常浇水及干旱胁迫下玉米幼苗的生长差异。结果显示,接种玉米来源的Cladosporium、Chaetomium、Beauveria对玉米幼苗的促生作用均优于Epicoccum、Alternaria,其中,LW29(Cladosporium sp.)、JN16(Cladosporium sp.)、NY15 (Chaetomium sp.)及YC1(Beauveria sp.)4个菌株对玉米幼苗的促生作用明显,可增强玉米幼苗的抗干旱能力。小麦来源的木霉菌JN-MY1(Trichoderma sp.)对玉米幼苗有一定的促生作用,但效果不如NY15、LW29、YC1及JN16。
In this study, 20 species of endophytic fungi isolated from maize and wheat tissues were inoculated into maize seedlings in a pot experiment under well-watered and drought stress simulated with 8% PEG-6000 in greenhouse. The differences in plant growth were analyzed. The results showed that the growth promoting effect of Cladosporium, Chaetomium and Beauveria from maize tissues on maize seedlings were superior to that of Epicoccum and Alternaria. LW29(Cladosporium sp.), JN16(Cladosporium sp.), NY15(Chaetomium sp.) and YC1(Beauveria sp.) promoted the growth of maize seedlings obviously both under well-water and drought stress conditions enhancing the drought resistance of maize seedlings. JN-MY1(Trichoderma sp.), a wheat-based Trichoderma, also exhibited growth-promoting effects on maize seedlings, but less than those of NY15, LW29, YC1 and JN16.
In this study, 20 species of endophytic fungi isolated from maize and wheat tissues were inoculated into maize seedlings in a pot experiment under well-watered and drought stress simulated with 8% PEG-6000 in greenhouse. The differences in plant growth were analyzed. The results showed that the growth promoting effect of Cladosporium, Chaetomium and Beauveria from maize tissues on maize seedlings were superior to that of Epicoccum and Alternaria. LW29(Cladosporium sp.), JN16(Cladosporium sp.), NY15(Chaetomium sp.) and YC1(Beauveria sp.) promoted the growth of maize seedlings obviously both under well-water and drought stress conditions enhancing the drought resistance of maize seedlings. JN-MY1(Trichoderma sp.), a wheat-based Trichoderma, also exhibited growth-promoting effects on maize seedlings, but less than those of NY15, LW29, YC1 and JN16.
引文
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[21] 胡美玲,郑勇,孙翔,等.内生真菌促进玉米幼苗的抗旱性研究[J].菌物学报,2017,36 (11):1556-1565.
[22] 张可可.玉米内生链格孢多样性及其生态功能研究[D].郑州:郑州大学,2017.
[23] 刘国丽,王娜,龚娜,等.干旱胁迫下植物内生菌提取物对玉米萌芽期抗旱性的影响[J].山东农业科学,2015,47 (10):39-41.
[24] Alwhibi M S,Hashem A,Abd_Allah E F,et al.Increased resistance of drought by Trichoderma harzianum fungal treatment correlates with increased secondary metabolites and proline content[J].Journal of Integrative Agriculture,2017,16 (8):1751-1757.
[2] Jacobson D J,Lefebvre S M,Ojerio R S,et al.Persistent,systemic,asymptomatic infections of Albugo candida,an oomycete parasite,detected in three wild crucifer species[J].Canadian Journal of Botany,1998,76 (5):739-750.
[3] Duckett J G,Russell J,Ligrone R.Basidiomycetous endophytes in jungerman-nialean (leafy) liverworts have novel cytology and species-specific host ranges:a cytological and experimental study[J].Canadian Journal of Botany,2006,84 (7):1075-1093.
[4] Nettles R,Watkins J,Ricks K,et al.Influence of pesticide seed treatments on rhizosphere fungal and bacterial communities and leaf fungal endophyte communities in maize and soybean[J].Applied Soil Ecology,2016,102:61-69.
[5] 王娜,杨镇,曹君,等.植物内生菌提取物对水稻生长发育的影响[J].吉林农业大学学报,2014,36(1):10-16.
[6] Hubbard M,Germida J J,Vujanovic V.Fungal endophytes enhance wheat and drought tolerance in terms of grain yield and second-generation seed viability[J].Journal of Applied Microbiology,2014,116 (1):109-122.
[7] Azad K,Kaminskyj S.A fungal endophyte strategy for mitigating the effect of salt and drought stress on plant growth[J].Symbiosis,2016,68 (1/2/3):73-78.
[8] Malinowski D P,Belesky D P.Adaptations of endophyte-infected cool-season grasses to environmental stresses:mechanisms of drought and mineral stress tolerance[J].Crop Science,2000,40 (4):923-940.
[9] Gusain Y S,Singh U S,Sharma A K.Enhance activity of stress related enzymes in rice (Oryza sativa L.) induced by plant growth promoting fungi under drought stress[J].African Journal of Agricultural Research,2014,9:1430-1434.
[10] Rai M,Rathod D,Agarka,G,et al.Fungal growth promoter endophytes:a pragmatic approach towards sustainable food and agriculture[J].Symbiosis,2014,62 (2):63-79.
[11] Mei C,Flinn B S.The use of beneficial microbial endophytes for plant biomass and stress tolerance improvement[J].Recent Patents Biotechnol.,2010,4 (1):81-95.
[12] 翁凌云.我国玉米生产现状及发展对策分析[J].中国食品与营养,2010(1):22-25.
[13] 范翠丽,陈景堂.玉米苗期及萌芽期抗旱性评定方法筛选[J].玉米科学,2007,15(3):114-117.
[14] Seema N,Hamayun M,Hussain A,et al.Endophytic fungi promote growth of Zea mays L.under PEG induced drought stress[J].Journal of Applied Environmental and Biological Sciences,2018,8 (3):95-101.
[15] Stone J K,Bacon C W,White J F,et al.An overview of endophytic microbes:endophytism defined[M]// Bacon C W,White J F (Eds.).Microbial Endophytes.Marcel Dekker,Inc.,N Y,2000.
[16] Eleena A T,Christiane B,Alexander I N,et al.Production of gibberellic acids by an orchid-associated Fusarium proliferatum strain[J].Fungal Genetics and Biology,2008,45 (10):1393-1403.
[17] 冯固,白灯莎,杨茂秋,等.不同生态型摩西球囊霉菌株对棉花耐盐性的影响[J].生态学报,2001,21(2):259-264.
[18] 姚彩艳,汪晓丽,盛海军,等.NaCl和PEG胁迫对玉米幼苗根系生长的影响[J].扬州大学学报 (农业与生命科学版),2007,28(4):42-46.
[19] Koide K,Osono T,Takeda H.Colonization and lignin decomposition of Camellia japonica leaf litter by endophytic fungi[J].Mycoscience,2005,46 (5):280-286.
[20] 王坚,刁治民,徐广,等.植物内生菌的研究概况及其应用[J].青海草业,2008(1):24-28.
[21] 胡美玲,郑勇,孙翔,等.内生真菌促进玉米幼苗的抗旱性研究[J].菌物学报,2017,36 (11):1556-1565.
[22] 张可可.玉米内生链格孢多样性及其生态功能研究[D].郑州:郑州大学,2017.
[23] 刘国丽,王娜,龚娜,等.干旱胁迫下植物内生菌提取物对玉米萌芽期抗旱性的影响[J].山东农业科学,2015,47 (10):39-41.
[24] Alwhibi M S,Hashem A,Abd_Allah E F,et al.Increased resistance of drought by Trichoderma harzianum fungal treatment correlates with increased secondary metabolites and proline content[J].Journal of Integrative Agriculture,2017,16 (8):1751-1757.