金银花及其制剂中掺伪山银花的快速鉴别研究
|
摘要
目的建立快速鉴别金银花及其制剂中掺伪山银花的方法。方法采用薄层色谱法,以灰毡毛忍冬皂苷乙为检测指标,以三氯甲烷-乙酸乙酯-甲醇-浓氨试液(0. 5∶0. 5∶10∶1)为展开剂,快速鉴别金银花及其制剂中掺伪的山银花。结果掺伪山银花在2%以上,即可准确判断。结论该方法简便、快捷,可用于快速鉴别金银花及其制剂中掺伪的山银花。
Objective To establish a rapid identification method of adulterated Lonicerae flos in Lonicerae japonicae flos and its preparations. Methods The adulterated Lonicerae flos in Lonicerae japonicae flos and its preparations was identified rapidly by TLC with macranthoidin B as identification of indicators, with a mixture of chloroform-acetic ether-methanol-concentrated ammonia solution( 0. 5 ∶ 0. 5 ∶ 10 ∶ 1) as the developing agent. Results The adulterated Lonicerae flos in more than 2% could be accurately identified.Conclusion The method is simple and quick, which can be used for rapid identification of adulterated Lonicerae flos in Lonicerae japonicae flos and its preparations.
Objective To establish a rapid identification method of adulterated Lonicerae flos in Lonicerae japonicae flos and its preparations. Methods The adulterated Lonicerae flos in Lonicerae japonicae flos and its preparations was identified rapidly by TLC with macranthoidin B as identification of indicators, with a mixture of chloroform-acetic ether-methanol-concentrated ammonia solution( 0. 5 ∶ 0. 5 ∶ 10 ∶ 1) as the developing agent. Results The adulterated Lonicerae flos in more than 2% could be accurately identified.Conclusion The method is simple and quick, which can be used for rapid identification of adulterated Lonicerae flos in Lonicerae japonicae flos and its preparations.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:30-31,221.
[2]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2000:177.
[3]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国化学工业出版社,2005:152-153,21-22.
[4]杜艳青,马斐,张静,等.小儿咳喘灵颗粒中金银花的质量分析[J].中成药,2015,37(12):2660-2664.
[5]马跃新,田小权,杨晓琼,等.金银花与山银花的薄层色谱鉴别[J].医药前沿,2014,33(11):351-352.
[6]笔雪艳,张清波,刘晓凤,等. 7种常用中药制剂中金银花与山银花的应用初探[J].药物分析杂志,2010,30(11):2208-2211.
[7]吴飞燕,冯宋岗,曾建国.金银花和山银花的鉴别与归属研究[J].中草药,2014,45(8):1150-1156.
[8]郑义,陈晓兰,丁宁,等.金银花和山银花有效成分测定及比较分析[J].甘肃农业大学学报,2015,50(4):170-174.
[9]代晶,罗玲,王佳慧,等. HPLC-UV-ELSD同时测定灰毡毛忍冬中9种成分的含量[J].中药材,2015,38(6):1217-1219.
[10]李红霞,王雪芹,李振国,等.不同产地金银花与山银花主要成分的含量比较[J].中国药房,2011,22(31):2935-2937.
[11]张湘东,宋伟峰,钟鸣,等.高效液相色谱串联质谱法鉴定山银花水提取液的化学成分[J].中国医药导报,2012,9(24):113-114.
[12]林永强,王淑华,徐丽华,等.金银花与山银花的鉴别方法研究[J].药学研究,2013,32(2):69-71.
[13]高珊,郭月秋,刘斯亮.现时金银花与山银花比较鉴别分析[J].中国医药科学,2017,7(1):52-55.
[2]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2000:177.
[3]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国化学工业出版社,2005:152-153,21-22.
[4]杜艳青,马斐,张静,等.小儿咳喘灵颗粒中金银花的质量分析[J].中成药,2015,37(12):2660-2664.
[5]马跃新,田小权,杨晓琼,等.金银花与山银花的薄层色谱鉴别[J].医药前沿,2014,33(11):351-352.
[6]笔雪艳,张清波,刘晓凤,等. 7种常用中药制剂中金银花与山银花的应用初探[J].药物分析杂志,2010,30(11):2208-2211.
[7]吴飞燕,冯宋岗,曾建国.金银花和山银花的鉴别与归属研究[J].中草药,2014,45(8):1150-1156.
[8]郑义,陈晓兰,丁宁,等.金银花和山银花有效成分测定及比较分析[J].甘肃农业大学学报,2015,50(4):170-174.
[9]代晶,罗玲,王佳慧,等. HPLC-UV-ELSD同时测定灰毡毛忍冬中9种成分的含量[J].中药材,2015,38(6):1217-1219.
[10]李红霞,王雪芹,李振国,等.不同产地金银花与山银花主要成分的含量比较[J].中国药房,2011,22(31):2935-2937.
[11]张湘东,宋伟峰,钟鸣,等.高效液相色谱串联质谱法鉴定山银花水提取液的化学成分[J].中国医药导报,2012,9(24):113-114.
[12]林永强,王淑华,徐丽华,等.金银花与山银花的鉴别方法研究[J].药学研究,2013,32(2):69-71.
[13]高珊,郭月秋,刘斯亮.现时金银花与山银花比较鉴别分析[J].中国医药科学,2017,7(1):52-55.