金银花不同部位化学成分的比较研究
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摘要
为了比较金银花茎、叶和花中的化学成分含量,采用了硫酸-蒽酮法、苯酚-硫酸法、2,6-二氯酚靛酚滴定法、考马斯亮蓝法、茚三酮呈色法和紫外分光光度法,分别测定了金银花茎、叶和花中的黄酮类物质、维生素C、绿原酸、可溶性蛋白质、游离氨基酸、可溶性糖和多糖的含量。结果表明:黄酮类化合物和维生素C含量均为叶>花>茎;而绿原酸、可溶性蛋白质和游离氨基酸含量均为:花>叶>茎,虽然花中含量最高,但叶和茎中也有一定的含量;可溶性糖:茎>花>叶;多糖含量:茎>叶>花。这为金银花的综合利用提供了一定的理论依据。
This study aims to compare the chemical constituents in stems,leaves and flowers of honeysuckle.The contents of flavonoids,vitamin C,chlorogenic acid,soluble protein,free amino acid,soluble sugar and polysaccharide are measured by sulfuric acid-anthrone method,phenol-sulfuric acid method,2,6-di chlorphenol indophenol titration method,Coomassie brilliant blue method,ninhydrin coloration method,and ultraviolet spectrophotometry.Results showed that the content of flavonoids and vitamin C in honeysuckle organs were leaf>flower>stem,the content of chlorogenic acid,soluble protein and free amino acid in honeysuckle organs were flower>leaf>stem,although the content in flower was the highest,there were also certain contents in leaves and stems;the soluble sugar content in honeysuckle organs was stem>flower>leaf,and the polysaccharide content was stem>leaf>flower.This study provides a basis for comprehensive utilization of honeysuckle.
This study aims to compare the chemical constituents in stems,leaves and flowers of honeysuckle.The contents of flavonoids,vitamin C,chlorogenic acid,soluble protein,free amino acid,soluble sugar and polysaccharide are measured by sulfuric acid-anthrone method,phenol-sulfuric acid method,2,6-di chlorphenol indophenol titration method,Coomassie brilliant blue method,ninhydrin coloration method,and ultraviolet spectrophotometry.Results showed that the content of flavonoids and vitamin C in honeysuckle organs were leaf>flower>stem,the content of chlorogenic acid,soluble protein and free amino acid in honeysuckle organs were flower>leaf>stem,although the content in flower was the highest,there were also certain contents in leaves and stems;the soluble sugar content in honeysuckle organs was stem>flower>leaf,and the polysaccharide content was stem>leaf>flower.This study provides a basis for comprehensive utilization of honeysuckle.
引文
[1]王国亮,朱信强,王金凤,等.豫北平原栽培金银花精油化学成分分析[J].中国中药杂志,1992,17(5):268-270.
[2]刘家欣,谷宜洁.湘西金银花挥发油化学成分研究[J].分析科学学报,1999,15(1):66-69.
[3]高玉敏,王名洲,王建平,等.金银花化学成分的研究[J].中草药,1995,26(11):568-569.
[4]黄丽瑛,吕植祯,李继彪等.中药金银花化学成分的研究[J].中草药,1996,27(11):645-647.
[5]张英华,肖寄平,易晓煜,等.不同产地金银花中绿原酸的含量比较[J].长春中医学院学报,1998,14(003):47-47.
[6]景小琦,武雪芬,雷敬卫.金银花枝叶中粗黄酮、无机元素和蛋白质含量测定[J].河南中医,2001,21(4):66-67.
[7]张雁冰,寇娴,王桂红,等.密县金银花及其茎、叶中绿原酸含量测定[J].河南医科大学学报,1999,34(2):36-36.
[8]周春晖,李俊.β-环糊精辅助提取金银花中总黄酮的工艺研究[J].中成药,2010,32(10):1796-1798.
[9]吴春霞,宋曼殳,阿不都拉·阿巴斯.小甘菊不同部位总黄酮含量测定及其薄层色谱的初步分析[J].食品科学,2007,28(7):430-433.
[10]丁既鹏,李世林.醇提法和超声波法提取金银花中黄酮类物质的研究[J].中国实验方剂学杂志,2005,11(4):64-64.
[11]林炎坤.常用的几种蒽酮比色定糖法的比较和改进[J].植物生理学通讯,1989(4):53-55.
[12]咸丰,李发胜.苯酚-硫酸法测定党参多糖含量[J].沈阳部队医药,2006,19(3):176-177.
[13]罗毅,潘细贵,刘刚,等.苯酚-硫酸法测定多糖含量显色方式的优选[J].中国中医药信息杂志,2005,12(1):45-46.
[14]肖文平,李娟.金银花中绿原酸的提取和含量测定[J].安徽农业科学,2011,39(35):21675-21676.
[15]李玉贤.金银花茎中绿原酸和黄酮类化合物含量测定[J].河南科学,1998,16(1):66-68.
[16]李明菊.食品中维生素C的测定方法研究[J].攀枝花学院学报,2012,29(5):109-110.
[17]孙义.水果和蔬菜中维生素C含量的测定方法综述[J].天津化工,2008,22(3):58-59.
[18]王风霞,黄玉琴,谢天柱等.测定果蔬中维生素C含量的方法比较[J].落叶果树,2013,45(2):8-11.
[19]李关荣,李天俊,冯建成.生物化学实验教程[M].1版.北京:中国农业大学出版社,2011.
[20]张永芳.茚三酮呈色法测定谷子种子中的游离氨基酸含量[J].种子,2014,33(1):111-113.
[2]刘家欣,谷宜洁.湘西金银花挥发油化学成分研究[J].分析科学学报,1999,15(1):66-69.
[3]高玉敏,王名洲,王建平,等.金银花化学成分的研究[J].中草药,1995,26(11):568-569.
[4]黄丽瑛,吕植祯,李继彪等.中药金银花化学成分的研究[J].中草药,1996,27(11):645-647.
[5]张英华,肖寄平,易晓煜,等.不同产地金银花中绿原酸的含量比较[J].长春中医学院学报,1998,14(003):47-47.
[6]景小琦,武雪芬,雷敬卫.金银花枝叶中粗黄酮、无机元素和蛋白质含量测定[J].河南中医,2001,21(4):66-67.
[7]张雁冰,寇娴,王桂红,等.密县金银花及其茎、叶中绿原酸含量测定[J].河南医科大学学报,1999,34(2):36-36.
[8]周春晖,李俊.β-环糊精辅助提取金银花中总黄酮的工艺研究[J].中成药,2010,32(10):1796-1798.
[9]吴春霞,宋曼殳,阿不都拉·阿巴斯.小甘菊不同部位总黄酮含量测定及其薄层色谱的初步分析[J].食品科学,2007,28(7):430-433.
[10]丁既鹏,李世林.醇提法和超声波法提取金银花中黄酮类物质的研究[J].中国实验方剂学杂志,2005,11(4):64-64.
[11]林炎坤.常用的几种蒽酮比色定糖法的比较和改进[J].植物生理学通讯,1989(4):53-55.
[12]咸丰,李发胜.苯酚-硫酸法测定党参多糖含量[J].沈阳部队医药,2006,19(3):176-177.
[13]罗毅,潘细贵,刘刚,等.苯酚-硫酸法测定多糖含量显色方式的优选[J].中国中医药信息杂志,2005,12(1):45-46.
[14]肖文平,李娟.金银花中绿原酸的提取和含量测定[J].安徽农业科学,2011,39(35):21675-21676.
[15]李玉贤.金银花茎中绿原酸和黄酮类化合物含量测定[J].河南科学,1998,16(1):66-68.
[16]李明菊.食品中维生素C的测定方法研究[J].攀枝花学院学报,2012,29(5):109-110.
[17]孙义.水果和蔬菜中维生素C含量的测定方法综述[J].天津化工,2008,22(3):58-59.
[18]王风霞,黄玉琴,谢天柱等.测定果蔬中维生素C含量的方法比较[J].落叶果树,2013,45(2):8-11.
[19]李关荣,李天俊,冯建成.生物化学实验教程[M].1版.北京:中国农业大学出版社,2011.
[20]张永芳.茚三酮呈色法测定谷子种子中的游离氨基酸含量[J].种子,2014,33(1):111-113.