留兰香挥发油熏蒸抑菌活性研究
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摘要
为了确定留兰香挥发油对植物病原真菌的生防效果,进一步探讨其抑菌机制,以明确其在植物真菌病害防控方面的应用前景,本研究采用菌丝生长速率法测定了留兰香挥发油对番茄灰霉病菌Botrytis cinerea、小麦赤霉病菌Fusarium graminearum、水稻纹枯病菌Rhizoctonia solani和莴苣菌核病菌Sclerotinia sclerotiorum 4种植物病原真菌的熏蒸抑制作用,并利用GC-MS分析了留兰香挥发油的化学组分。结果显示,留兰香挥发油对4种供试真菌均有强烈的熏蒸抑制作用,EC_(50)均低于7.0μL/L。挥发油处理后番茄灰霉病菌菌丝畸变、表面塌陷,细胞膜被破坏、原生质泄漏,细胞质结构呈消解状,线粒体受损。通过GC-MS从留兰香挥发油中分离出30个组分,鉴定出其中24个组分,占挥发油总量的94.918%,其主要成分有L-香芹酮(57.468%)、D-柠檬烯(8.976%)等。研究表明,留兰香挥发油是理想的绿色熏蒸杀菌资源。
The biocontrol potential of essential oils from Mentha spicata against plant pathogenic fungi was investigated to explore their applications in the prevention and control of plant fungal diseases. In this study, the fumigant antifungal activities of essential oils from M. spicata against four plant pathogenic fungi including Botrytis cinerea, Fusarium graminearum, Rhizoctonia solani and Sclerotinia sclerotiorum were determined by mycelial growth rate method; the influences of essential oils on mycelia of B.cinerea were observed, and chemical constituents of essential oils were analyzed by GC-MS.The results showed that the essential oils from M. spicata had strong fumigation activities against all tested pathogenic fungi and EC_(50) values were lower than 7.0 μL/L. After treatment with essential oils, aberration and surface depression in mycelia of B.cinerea, cell membrane destruction and cytoplasmic exosmosis, cytoplasmic structure digestion, and mitochondrial damage were observed. Totally 30 constituents were isolated, and 24 were identified by GC-MS, accounting for 94.918% of the total essential oils. The main chemical constituents included L-carvone(57.468%), D-limonene(8.976%) and so on. In conclusion, the essential oils from M. spicata were ideal green fumigant antifungal resources.
The biocontrol potential of essential oils from Mentha spicata against plant pathogenic fungi was investigated to explore their applications in the prevention and control of plant fungal diseases. In this study, the fumigant antifungal activities of essential oils from M. spicata against four plant pathogenic fungi including Botrytis cinerea, Fusarium graminearum, Rhizoctonia solani and Sclerotinia sclerotiorum were determined by mycelial growth rate method; the influences of essential oils on mycelia of B.cinerea were observed, and chemical constituents of essential oils were analyzed by GC-MS.The results showed that the essential oils from M. spicata had strong fumigation activities against all tested pathogenic fungi and EC_(50) values were lower than 7.0 μL/L. After treatment with essential oils, aberration and surface depression in mycelia of B.cinerea, cell membrane destruction and cytoplasmic exosmosis, cytoplasmic structure digestion, and mitochondrial damage were observed. Totally 30 constituents were isolated, and 24 were identified by GC-MS, accounting for 94.918% of the total essential oils. The main chemical constituents included L-carvone(57.468%), D-limonene(8.976%) and so on. In conclusion, the essential oils from M. spicata were ideal green fumigant antifungal resources.
引文
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[14] 邹正耀,张红英,王学兵,等.留兰香挥发油提取及其体外抗植物真菌研究[J].中国农学通报,2011,27(7):262-265.
[15] 陈利军,智亚楠,王国君,等.土荆芥花序和叶挥发油的抑菌作用及组分分析[J].植物保护,2014,40(5):40-43.
[16] 陈利军,智亚楠,王国君,等.土荆芥果实挥发油的抑菌活性及其组分分析[J].河南农业科学,2015,44(1):70-76.
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[21] 郝静梅,盛冉,孙志高,等.柠檬烯抗菌性研究进展[J].食品与发酵工业,2017,43(2):274-278.
[22] 李亚茹,周林燕,李淑荣,等.植物精油对果蔬中微生物的抑菌效果及作用机理研究进展[J].食品科学,2014,35(11):325-329.
[23] 李文茹,施庆珊,谢小保,等.植物精油化学成分及其抗菌活性的研究进展[J].微生物学报,2016,43(6):1339-1344.
[24] 李燕君,孔维军,李梦华,等.植物精油抑制真菌及真菌毒素的研究进展[J].中草药,2016,47(11):2011-2018.
[25] 丁月,叶仁凤,欧昌荣,等.加拿大一枝黄花精油的提取及对灰霉菌抑制作用的研究[J].食品工业科技,2015,36(6):153-156.
[26] SHAO X F, CHENG S, WANG H F, et al. The possible mechanism of antifungal action of tea tree oil on Botrytis cinerea [J].Journal of Applied Microbiology,2013,114(6):1642-1649.
[27] 王一,杨雅婷,杨静,等.物种多样性与病虫草害管理研究进展[J].中国生物防治学报,2015,31(5):801-810.
[28] 向慧敏,章家恩,罗明珠,等.水稻与水芹间作栽培对水稻病虫草害和产量的影响[J].生态与农村环境学报,2013,29(1):58-63.
[29] 江兵兵,张彧,郭存武,等.韭菜和辣椒间作对辣椒疫病的防治效果及其化感机理[J].植物保护学报,2017,44(1):145-151.
[30] 奚同行,何长高.果园套种留兰香的生物防治作用与生态效益分析[J].南昌水专学报,1999,18(1):41-46.
[2] 陈建烟,李永裕,吴少华.植物精油生物活性作用机理研究进展[J].天然产物研究与开发,2012,24(9):1312-1318.
[3] 陈利军,陈月华,周巍,等.河南鸡公山小鱼仙草挥发油的抑菌作用及组分分析[J].南方农业学报,2016(11):1875-1879.
[4] 高宇,孙晓玲,金珊,等.绿叶挥发物及其生态功能研究进展[J].农学学报,2012,2(4):11-23.
[5] TUMLINSON H. The importance of volatile organic compounds in ecosystem functioning [J]. Journal of Chemical Ecology, 2014, 40(3): 212-213.
[6] 中国科学院中国植物志编辑委员会. 中国植物志第66卷[M]. 北京: 科学出版社, 1977: 271.
[7] ZAIDI S, DAHIYA P. In vitro antimicrobial activity, phytochemical analysis and total phenolic content of essential oil from Mentha spicata and Mentha piperita[J]. International Food Research Journal, 2015, 22(6): 2440-2445.
[8] SHAHBAZI Y. Chemical composition and in vitro antibacterial activity of Mentha spicata essential oil against common food-borne pathogenic bacteria[J]. Journal of Pathogens, 2015, 2015(2): 916305-916310.
[9] 潘旭迟,徐剑锋,傅昱晟,等.3种植物挥发油抑制食源性细菌生长活性成分及机理[J].食品科学,2017,38(13):143-149.
[10] CHRYSARGYRIS A, XYLIA P, BOTSARIS G, et al. Antioxidant and antibacterial activities, mineral and essential oil composition of spearmint (Mentha spicata L.) affected by the potassium levels [J]. Industrial Crops and Products, 2017, 103(10): 202-212.
[11] SNOUSSI M, NOUMI E, TRABELSI N, et al. Mentha spicata essential oil: chemical composition, antioxidant and antibacterial activities against planktonic and biofilm cultures of Vibrio spp. strains [J]. Molecules, 2015, 20: 14402-14424.
[12] KEDIA A, DWIVEDY A K, JHA D K, et al. Efficacy of Mentha spicata essential oil in suppression of Aspergillus flavus and aflatoxin contamination in chickpea with particular emphasis to mode of antifungal action[J]. Protoplasma, 2016, 253(3): 647-653.
[13] OJEWUMI M E, ADEDOKUM S O, OMODARA O J, et al. Phytochemical and antimicrobial of the leaf oil extract of Mentha spicata and its efficacy in repelling mosquito [J]. International Journal of Pharmaceutical Research and Allied Sciences, 2017, 6(4): 17-27.
[14] 邹正耀,张红英,王学兵,等.留兰香挥发油提取及其体外抗植物真菌研究[J].中国农学通报,2011,27(7):262-265.
[15] 陈利军,智亚楠,王国君,等.土荆芥花序和叶挥发油的抑菌作用及组分分析[J].植物保护,2014,40(5):40-43.
[16] 陈利军,智亚楠,王国君,等.土荆芥果实挥发油的抑菌活性及其组分分析[J].河南农业科学,2015,44(1):70-76.
[17] 康振生.植物病原真菌的超微结构[M].北京:中国科学技术出版社,1995:1-29.
[18] 付磊磊,李双阳,何军,等.香芹酮和薄荷醇手性异构体对淡色库蚊熏蒸及趋避活性[J].昆虫学报,2016,59(7):732-738.
[19] REGNIER T, COMBRINCK S, PLOOY W D, et al. Evaluation of Lippia scaberrima essential oil and some pure terpenoid constituents as postharvest mycobiocides for avocado fruit [J]. Postharvest Bioloby and Technology,2010,57(3):176-182.
[20] BOONRUANG K, KERDDONFAG N, CHINSIRIKUL W, et al. Antifungal effect of poly(lactic acid) films containing thymol and R-(-)-carvone against anthracnose pathogens isolated from avocado and citrus [J].Food Control,2017,78(2):85-93.
[21] 郝静梅,盛冉,孙志高,等.柠檬烯抗菌性研究进展[J].食品与发酵工业,2017,43(2):274-278.
[22] 李亚茹,周林燕,李淑荣,等.植物精油对果蔬中微生物的抑菌效果及作用机理研究进展[J].食品科学,2014,35(11):325-329.
[23] 李文茹,施庆珊,谢小保,等.植物精油化学成分及其抗菌活性的研究进展[J].微生物学报,2016,43(6):1339-1344.
[24] 李燕君,孔维军,李梦华,等.植物精油抑制真菌及真菌毒素的研究进展[J].中草药,2016,47(11):2011-2018.
[25] 丁月,叶仁凤,欧昌荣,等.加拿大一枝黄花精油的提取及对灰霉菌抑制作用的研究[J].食品工业科技,2015,36(6):153-156.
[26] SHAO X F, CHENG S, WANG H F, et al. The possible mechanism of antifungal action of tea tree oil on Botrytis cinerea [J].Journal of Applied Microbiology,2013,114(6):1642-1649.
[27] 王一,杨雅婷,杨静,等.物种多样性与病虫草害管理研究进展[J].中国生物防治学报,2015,31(5):801-810.
[28] 向慧敏,章家恩,罗明珠,等.水稻与水芹间作栽培对水稻病虫草害和产量的影响[J].生态与农村环境学报,2013,29(1):58-63.
[29] 江兵兵,张彧,郭存武,等.韭菜和辣椒间作对辣椒疫病的防治效果及其化感机理[J].植物保护学报,2017,44(1):145-151.
[30] 奚同行,何长高.果园套种留兰香的生物防治作用与生态效益分析[J].南昌水专学报,1999,18(1):41-46.