圆齿野鸦椿花青素提取工艺优化及抗氧化活性评价
|
摘要
以圆齿野鸦椿果皮为原料,在单因素试验的基础上,设计四因素三水平的组合试验,建立了超声辅助提取果皮花青素的最佳工艺条件,即超声温度40℃,时间40 min,功率300 W,料液比1∶6.在此条件下花青素提取量为(3.740±0.058) mg·g~(-1).1,1-二苯基-2-苦肼自由基(DPPH·)、羟基自由基(·OH)、超氧自由基■活性评价结果表明,圆齿野鸦椿花青素具有明显的抗氧化活性,花青素质量浓度为0.1～3.2 mg·mL~(-1)时,其抗氧化活性与浓度呈线性相关.表明超声辅助提取圆齿野鸦椿果皮花青素,效果优,时间短,浸提液用量少.
To find the optimal process for anthocyanin extraction from Euscaphis konishiifruit, A L_9(3~4) orthogonal experiment, consisting of 4 factors at 3 levels, were developed based on single factor experiment. The results showed that the optimum extraction condition was to add solid-liquid ingredients at the ratio of 1∶6, and extract ultrasonically for 40 min at 40 ℃ under the power of 300 W. Under this condition, the extraction of anthocyanin was(3.74±0.058)mg·g~(-1). Subsequently, in vitro antioxidant activities of anthocyanin were evaluated by scavenging 1, 1-diphenyl-2-picrylhydrazyl radica(DPPH·), hydroxyl radical(OH·) and superoxide radical ■. The results proved that E.konishii anthocyanin possessed significant antioxidant capacity. The antioxidant activity was linearly positively correlated with anthocyanin concentration within the range of 0.1-3.2 mg·mL~(-1). In summary, ultrasonic-assisted extraction was an effective method for extracting anthocyanin from E.konishiipeal with less extractant and processing time.
To find the optimal process for anthocyanin extraction from Euscaphis konishiifruit, A L_9(3~4) orthogonal experiment, consisting of 4 factors at 3 levels, were developed based on single factor experiment. The results showed that the optimum extraction condition was to add solid-liquid ingredients at the ratio of 1∶6, and extract ultrasonically for 40 min at 40 ℃ under the power of 300 W. Under this condition, the extraction of anthocyanin was(3.74±0.058)mg·g~(-1). Subsequently, in vitro antioxidant activities of anthocyanin were evaluated by scavenging 1, 1-diphenyl-2-picrylhydrazyl radica(DPPH·), hydroxyl radical(OH·) and superoxide radical ■. The results proved that E.konishii anthocyanin possessed significant antioxidant capacity. The antioxidant activity was linearly positively correlated with anthocyanin concentration within the range of 0.1-3.2 mg·mL~(-1). In summary, ultrasonic-assisted extraction was an effective method for extracting anthocyanin from E.konishiipeal with less extractant and processing time.
引文
[1] 侯锐,陈琦,王利,等.花青素及其生物活性的研究进展[J].现代生物医学进展,2015,15(28):5 590-5 593.
[2] 左玉,田芳.花青素稳定性研究进展[J].粮食与油脂,2014,27(7):1-5.
[3] REIN M. Copigmentation Reactions and Color Stability of Berry Anthocyanins[D]. Finland: University of Helsinki, 2005.
[4] HOU Z, QIN P, ZHANG Y, et al. Identification of anthocyanins isolated from black rice (Oryza sativa, L.) and their degradation kinetics[J]. Food Research International, 2013,50(2):691-697.
[5] 方文培.中国植物志(46卷)[M].北京:科学出版社,1981(46):23-24.
[6] 范敏.赣州大规模引种圆齿野鸦椿[N].中国花卉报,2014-04-24(003).
[7] 李玉平,邹双全,何碧珠.圆齿野鸦椿种子外植体的快繁体系[J].福建农林大学学报(自然科学版),2010,39(5):480-483.
[8] 何碧珠,何官榕,邹双全.圆齿野鸦椿叶片的植株再生及快速繁殖[J].福建农林大学学报(自然科学版),2010,39(3):257-262.
[9] 孙维红,袁雪艳,吴玲娇,等.圆齿野鸦椿开花动态特征与繁育系统[J].植物生理学报,2017,53(12):2 215-2 221.
[10] 覃嘉佳,胡滨,黄焱辉,等.圆齿野鸦椿种子内含物的提取、分离以及生物测定[J].安徽农业科学,2011,39(32):19 693-19 694.
[11] 邹小兴,刘宇,邹双全,等.圆齿野鸦椿叶及枝化学成分初步研究[J].中国野生植物资源,2016,35(1):70-72.
[12] 刘迪栋,文旭,汤勇,等.野鸦椿水提物对大鼠慢性肝纤维化的影响[J].当代医学,2013,19(15):33-35.
[13] 葛翠莲,黄春辉,徐小彪.果实花青素生物合成研究进展[J].园艺学报,2012,39(9):1 655-1 664.
[14] SAURE M C. External control of anthocyanin formation in apple[J]. Scientia Horticulturae, 1990,42(3):181-218.
[15] 汪良驹,王中华,李志强,等.L-谷氨酸促进富士苹果花青素积累的效应[J].果树学报,2006,(2):154-160.
[16] 齐秀娟,徐善坤,林苗苗,等.红肉猕猴桃果实着色机制研究进展[J].果树学报,2015,32(6):1 232-1 240.
[17] JAAKOLA L. New insights into the regulation of anthocyanin biosynthesis in fruits[J]. Trends in Plant Science, 2013,18(9):477.
[18] 袁雪艳,邹小兴,黄维,等.圆齿野鸦椿朔果着色及呈色分析[J].经济林研究,2018,36(3):100-106.
[19] YUAN X, SUN W, ZOU X, et al. Sequencing of Euscaphis konishii endocarp transcriptome points to molecular mechanisms of endocarp coloration[J]. Int J Mol Sci, 2018,19:3 209.
[20] 陈成花,张婧,陈海燕,等.蓝莓果渣花色苷超声提取工艺优化及组成分析[J].食品科技,2016,41(4):192-199.
[21] 陈小婕,阴文娅.植物中花青素提取方法探讨[J].食品工业科技,2013,34(2):395-399.
[22] 徐渊金,杜琪珍.花色苷分离鉴定方法及其生物活性[J].食品与发酵工业,2006(3):67-72.
[23] CHEN F, SUN Y, ZHAO G, et al. Optimization of ultrasound-assisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography-mass spectrometry[J]. Ultrasonics Sonochemistry, 2007,14(6):767-78.
[24] 谭莉,陈瑞战,彭雨沙,等.蓝莓花青素提取工艺优化及抗氧化活性评价[J].食品工业,2017,38(8):136-141.
[2] 左玉,田芳.花青素稳定性研究进展[J].粮食与油脂,2014,27(7):1-5.
[3] REIN M. Copigmentation Reactions and Color Stability of Berry Anthocyanins[D]. Finland: University of Helsinki, 2005.
[4] HOU Z, QIN P, ZHANG Y, et al. Identification of anthocyanins isolated from black rice (Oryza sativa, L.) and their degradation kinetics[J]. Food Research International, 2013,50(2):691-697.
[5] 方文培.中国植物志(46卷)[M].北京:科学出版社,1981(46):23-24.
[6] 范敏.赣州大规模引种圆齿野鸦椿[N].中国花卉报,2014-04-24(003).
[7] 李玉平,邹双全,何碧珠.圆齿野鸦椿种子外植体的快繁体系[J].福建农林大学学报(自然科学版),2010,39(5):480-483.
[8] 何碧珠,何官榕,邹双全.圆齿野鸦椿叶片的植株再生及快速繁殖[J].福建农林大学学报(自然科学版),2010,39(3):257-262.
[9] 孙维红,袁雪艳,吴玲娇,等.圆齿野鸦椿开花动态特征与繁育系统[J].植物生理学报,2017,53(12):2 215-2 221.
[10] 覃嘉佳,胡滨,黄焱辉,等.圆齿野鸦椿种子内含物的提取、分离以及生物测定[J].安徽农业科学,2011,39(32):19 693-19 694.
[11] 邹小兴,刘宇,邹双全,等.圆齿野鸦椿叶及枝化学成分初步研究[J].中国野生植物资源,2016,35(1):70-72.
[12] 刘迪栋,文旭,汤勇,等.野鸦椿水提物对大鼠慢性肝纤维化的影响[J].当代医学,2013,19(15):33-35.
[13] 葛翠莲,黄春辉,徐小彪.果实花青素生物合成研究进展[J].园艺学报,2012,39(9):1 655-1 664.
[14] SAURE M C. External control of anthocyanin formation in apple[J]. Scientia Horticulturae, 1990,42(3):181-218.
[15] 汪良驹,王中华,李志强,等.L-谷氨酸促进富士苹果花青素积累的效应[J].果树学报,2006,(2):154-160.
[16] 齐秀娟,徐善坤,林苗苗,等.红肉猕猴桃果实着色机制研究进展[J].果树学报,2015,32(6):1 232-1 240.
[17] JAAKOLA L. New insights into the regulation of anthocyanin biosynthesis in fruits[J]. Trends in Plant Science, 2013,18(9):477.
[18] 袁雪艳,邹小兴,黄维,等.圆齿野鸦椿朔果着色及呈色分析[J].经济林研究,2018,36(3):100-106.
[19] YUAN X, SUN W, ZOU X, et al. Sequencing of Euscaphis konishii endocarp transcriptome points to molecular mechanisms of endocarp coloration[J]. Int J Mol Sci, 2018,19:3 209.
[20] 陈成花,张婧,陈海燕,等.蓝莓果渣花色苷超声提取工艺优化及组成分析[J].食品科技,2016,41(4):192-199.
[21] 陈小婕,阴文娅.植物中花青素提取方法探讨[J].食品工业科技,2013,34(2):395-399.
[22] 徐渊金,杜琪珍.花色苷分离鉴定方法及其生物活性[J].食品与发酵工业,2006(3):67-72.
[23] CHEN F, SUN Y, ZHAO G, et al. Optimization of ultrasound-assisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography-mass spectrometry[J]. Ultrasonics Sonochemistry, 2007,14(6):767-78.
[24] 谭莉,陈瑞战,彭雨沙,等.蓝莓花青素提取工艺优化及抗氧化活性评价[J].食品工业,2017,38(8):136-141.