工厂化生产金针菇菌渣栽培糙皮侧耳的应用分析
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摘要
研究了生产用糙皮侧耳菌株对工厂化生产金针菇菌渣的适应性,菌渣不同发酵处理方式和复配基质对糙皮侧耳生长发育的影响。结果表明,不同糙皮侧耳菌株对金针菇菌渣的适应性不同,各菌株以菌渣为基质的生物转化率均显著低于棉籽壳基质配方,但降低幅度存在较大差异;金针菇菌渣分别发酵0、3 d、5 d,随着发酵时间延长,菌袋污染率呈降低趋势,糙皮侧耳产量呈升高趋势;单一金针菇菌渣为主料栽培糙皮侧耳,各潮菇的产量均显著低于棉籽壳基质配方,第2潮～第4潮菇的产量降低更加明显,金针菇菌渣与棉籽壳、玉米芯复配基质的产量显著提高。
The adaptability of Pleurotus ostreatus strains to spent industrialized Flammulina velutipes substrate was analyzed,and the effects of different spent substrate fermentation treatments and mixed substrates on the growth of P. ostreatus were studied. The results showed that the adaptability of different P. ostreatus strains to spent substrate of F. velutipes was different, the biological efficiency of each P. ostreatus strains cultivated on spent substrate were significantly lower than that cultivated on cotton seed hull substrate, but there were significant differences in the extent of reduction. The spent substrate f F. velutipes was fermented 0, 3 d and 5 d respectively, with the prolonging of fermentation time, the contamination rate of P. ostreatus bags was decreasing, and the yield of P. ostreatus was increasing. The yield of each flush of P. ostreatus cultivated on spent substrate f F.velutipes was significantly lower than that cultivated on the cotton seed hull substrate, the yields of the 2nd to the 4th flushes of P. ostreatus were reduced more obviously. The yield increased significantly when spent substrate of F. velutipes was used in combination with cotton seed hull and corncob.
The adaptability of Pleurotus ostreatus strains to spent industrialized Flammulina velutipes substrate was analyzed,and the effects of different spent substrate fermentation treatments and mixed substrates on the growth of P. ostreatus were studied. The results showed that the adaptability of different P. ostreatus strains to spent substrate of F. velutipes was different, the biological efficiency of each P. ostreatus strains cultivated on spent substrate were significantly lower than that cultivated on cotton seed hull substrate, but there were significant differences in the extent of reduction. The spent substrate f F. velutipes was fermented 0, 3 d and 5 d respectively, with the prolonging of fermentation time, the contamination rate of P. ostreatus bags was decreasing, and the yield of P. ostreatus was increasing. The yield of each flush of P. ostreatus cultivated on spent substrate f F.velutipes was significantly lower than that cultivated on the cotton seed hull substrate, the yields of the 2nd to the 4th flushes of P. ostreatus were reduced more obviously. The yield increased significantly when spent substrate of F. velutipes was used in combination with cotton seed hull and corncob.
引文
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[14]Xian-lu Zeng,Fei Han,Jing-li Ye,et al.Recycling spent Pleurotus eryngii substrate supplemented with Tenebrio molitor feces for cultivation of Agrocybe chaxingu[J].International Journal of Recycling of Organic Waste in Agriculture,2017,6(4):275-280.
[15]王庆武,安秀荣,李秀梅,等.金针菇菌渣栽培糙皮侧耳配方试验[J].山东农业科学,2012,44(9):56-58.
[16]杨鹏,韩建东,万鲁长,等.利用杏鲍菇菌渣栽培糙皮侧耳试验[J].中国食用菌,2015,34(5):14-16.
[17]Economou CN,Diamantopoulou PA,Philippoussis AN.Valorization of spent oyster mushroom substrate and laccase recovery through successive solid state cultivation of Pleurotus,Ganoderma,and Lentinula strains[J].Applied Microbiology and Biotechnology,2017,101(12):5213-5222.
[18]Phan CW,Sabaratnam V.Potential uses of spent mushroom substrate and its associated lignocellulosic enzymes[J].Applied Microbiology and Biotechnology,2012,96(4):863-873.
[2]Dilena G,Vivanti V,Quaglia GB.Amino acid composition of wheat milling by products after bioconversion by edible fungi mycelia[J].Nahrung-Food,1997,41(5):285-288.
[3]宫志远,韩建东,任鹏飞,等.工厂化金针菇菌糠栽培秀珍菇配方筛选试验[J].中国食用菌,2010,29(4):14-16.
[4]韩建东,宫志远,任海霞,等.利用金针菇工厂化生产的菌渣栽培洛巴伊大口蘑[J].食用菌学报,2011,18(3):39-41.
[5]韩建东,万鲁长,杨鹏,等.刺芹侧耳菌渣对肺形侧耳(秀珍菇)生长和营养成分的影响[J].菌物学报,2014,33(2):433-439.
[6]李正鹏,余昌霞,黄建春,等.三种食用菌菌渣部分替代废棉栽培草菇[J].食用菌学报,2016,23(1):27-30.
[7]Mamiro DP,Royse DJ.The influence of spawn type and strain on yield,size and mushroom solids content of Agaricus bisporus produced on non-composted and spent mushroom compost[J].Bioresource Technology,2008,99(8):3205-3212.
[8]González Matute R,Figlas D,Curvetto N.Agaricus blazei production on non-composted substrates based on sunflower seed hulls and spent oyster mushroom substrate[J].World Journal of Microbiology and Biotechnology,2011,27(6):1331-1339.
[9]Poppe J.Use of agricultural waste materials in the cultivation of mushrooms[J].Mushroom Science,2000(15):3-23.
[10]Royse DJ.Recycling of spent shiitake substrate for production of the oyster mushroom Pleurotus sajor-caju[J].Applied Microbiology and Biotechnology,1992,38(2):179-182.
[11]Singh RN,Bhandari TPS,Adhikari KS,et al.Physiochemical parameters of casing soil in relation to yield of button mushroom(Agaricus brunnescens)[J].Indian Journal of Mycology and Plant Pathology,1992,22(2):160-164.
[12]Sharma HSS,Furlan A,Lyons G.Comparative assessment of chelated spent mushroom substrates as casing material for the production of Agaricus bisporus[J].Applied Microbiology and Biotechnology,1999,52(3):366-372.
[13]Siddhant,Singh CS.Recycling of spent oyster mushroom substrate to recover additional value[J].Kathmandu University Journal of Science,Engineering and Technology,2009,5(2):66-71.
[14]Xian-lu Zeng,Fei Han,Jing-li Ye,et al.Recycling spent Pleurotus eryngii substrate supplemented with Tenebrio molitor feces for cultivation of Agrocybe chaxingu[J].International Journal of Recycling of Organic Waste in Agriculture,2017,6(4):275-280.
[15]王庆武,安秀荣,李秀梅,等.金针菇菌渣栽培糙皮侧耳配方试验[J].山东农业科学,2012,44(9):56-58.
[16]杨鹏,韩建东,万鲁长,等.利用杏鲍菇菌渣栽培糙皮侧耳试验[J].中国食用菌,2015,34(5):14-16.
[17]Economou CN,Diamantopoulou PA,Philippoussis AN.Valorization of spent oyster mushroom substrate and laccase recovery through successive solid state cultivation of Pleurotus,Ganoderma,and Lentinula strains[J].Applied Microbiology and Biotechnology,2017,101(12):5213-5222.
[18]Phan CW,Sabaratnam V.Potential uses of spent mushroom substrate and its associated lignocellulosic enzymes[J].Applied Microbiology and Biotechnology,2012,96(4):863-873.