离子色谱-脉冲安培法测定芦荟多糖中7种单糖的含量
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摘要
采用水提醇沉法提取芦荟鲜叶中的多糖,经三氟乙酸水解,利用Dionex CarboPac PA10高效阴离子色谱柱分离,氢氧化钠梯度淋洗,积分脉冲安培检测,建立了芦荟多糖中岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖和木糖7种常见单糖的测定方法。结果表明:7种单糖在线性范围内的相关系数(R~2)均高于0.997,检出限为0.007～0.024 mg/L,加标回收率为97.5%～102.5%,相对标准偏差(RSD)为0.1%～4.8%。该法简单、快速、灵敏、准确,可用于芦荟多糖中单糖含量的测定和多糖组成的研究。
Aloe polysaccharide was extracted by water extraction and ethanol precipitation. It was hydrolyzed by trifluoroacetic acid, eluted by a sodium hydroxide gradient on a Dionex CarboPac PA10 column, and detected using a pulsed amperometric detector. This method was used to determine the fucose, rhamnose, arabinose, galactose, glucose, mannose, and xylose in aloe polysaccharide. The results showed that the correlation coefficients(R~2) of all the seven target compounds were more than 0.997 in the linear range, and the limits of detection were between 0.007 mg/L and 0.024 mg/L. The recoveries were in the range 97.5%-102.5% with the relative standard deviations in the range 0.1%-4.8%. The method is simple, rapid, sensitive, and accurate for the determination of the monosaccharide content and the constitution of aloe polysaccharides.
Aloe polysaccharide was extracted by water extraction and ethanol precipitation. It was hydrolyzed by trifluoroacetic acid, eluted by a sodium hydroxide gradient on a Dionex CarboPac PA10 column, and detected using a pulsed amperometric detector. This method was used to determine the fucose, rhamnose, arabinose, galactose, glucose, mannose, and xylose in aloe polysaccharide. The results showed that the correlation coefficients(R~2) of all the seven target compounds were more than 0.997 in the linear range, and the limits of detection were between 0.007 mg/L and 0.024 mg/L. The recoveries were in the range 97.5%-102.5% with the relative standard deviations in the range 0.1%-4.8%. The method is simple, rapid, sensitive, and accurate for the determination of the monosaccharide content and the constitution of aloe polysaccharides.
引文
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