绿衣枳壳中枳属苷的提取工艺研究
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摘要
目的优选绿衣枳壳中枳属苷的最佳提取工艺。方法高效液相色谱法测定绿衣枳壳中枳属苷的含量,以枳属苷的含量为指标,应用单因素试验结合正交试验法优化绿衣枳壳中枳属苷的提取工艺。结果绿衣枳壳中枳属苷的最佳提取工艺为加入6倍量90%乙醇,提取3次,每次1 h。结论该提取工艺方法简单、稳定可行,可以为绿衣枳壳中枳属苷的大规模化生产工艺提供理论依据。
Objective: To study the optimum extraction of poncirin from Fructus Ponciri Trifoliatae. Methods:The content of poncirin, as the index, in Fructus Ponciri Trifoliatae were determined by HPLC, using single factor test combined with orthogonal experiment, the extraction process of Fructus Ponciri Trifoliatae in Poncirin was optimized. Results: The best extraction process of Fructus Ponciri Trifoliatae in poncirin was adding 6 times amount of 90% ethanol, extracting for 3 times, 1h each time. Conclusion: This method is simple, stable, reliable and can provide solid basis for large scale extraction of l poncirin from Fructus Ponciri Trifoliatae.
Objective: To study the optimum extraction of poncirin from Fructus Ponciri Trifoliatae. Methods:The content of poncirin, as the index, in Fructus Ponciri Trifoliatae were determined by HPLC, using single factor test combined with orthogonal experiment, the extraction process of Fructus Ponciri Trifoliatae in Poncirin was optimized. Results: The best extraction process of Fructus Ponciri Trifoliatae in poncirin was adding 6 times amount of 90% ethanol, extracting for 3 times, 1h each time. Conclusion: This method is simple, stable, reliable and can provide solid basis for large scale extraction of l poncirin from Fructus Ponciri Trifoliatae.
引文
[1]南京中医药大学.中药大辞典[M].2版.上海:上海科技出版社,2006:2245-2246.
[2]蔡逸平,曹岚,范崔生.枳壳、枳实类药材的品种考证和资源应用的调查研究[J].江西中医学院学报,1998,10(4):184-186.
[3]潘馨,谢秋情,李煌诚.HPLC法同时测定绿衣枳壳中3种黄酮类化合物的含量[J].药物分析杂志,2015,35(4):666-668.
[4]李煌,张梦云.不同采收期绿衣枳壳中柚皮苷的含量测定[J].中国中医药信息杂志,2008,15(7):43-44.
[5]欧德銮,张振.枸橘药材质量标准的研究[J].海峡药学,2009,21(7):74-76.
[6]潘馨,梁鸣.绿衣枳实挥发油的GC-MS分析[J].中药新药与临床药,2004,1(6):415-416.
[7]胡源祥,谭舒舒,陈海芳,等.绿衣枳壳中葡萄内酯的含量高效液相色谱法测定[J].时珍国医国药,2016,27(8):1810-1811.
[8]毕琼瑛,施大文.枸橘质量研究[J].中药通报,1988,13(11):34-36.
[9]董晶,梁兆昌,陈海芳,等.HPLC法同时测定不同采收期江枳壳中辛弗林和N-甲基酪胺的含量[J].井冈山大学学报:自然科学版,2016,37(2):92-96.
[10]李增,陈灵芝,刘晨.枸橘苷抑制血管生成作用研究[J].时珍国医国药,2016,27(2):277-279.
[11]Jong-Bin Kim,Ah-Reum Han,Eun-Young Park,et al.inhibition of induced io NS,COX-2 and cytokines expression by poncirin through the NF-ΚB Inactivation in RAW 264.7 macrophage cells[J].Biological and Pharmaceutical Bulletin,2007,30(12):2345-2351.
[12]Dong-Hyun K I M,Eun-Ah B A E,Han M J.Anti-Helicobacter pylori activity of the metabolites of poncirin from Poncirus trifoliata by human intestinal bacteria[J].Biological and Pharmaceutical Bulletin,1999,22(4):422-424.
[13]Lee J H,Lee S H,Kim Y S,et al.Protective effects of neohesperidin and poncirin isolated from the fruits of Poncirus trifoliata on potential gastric disease[J].Phytotherapy Research,2009,23(12):1748-1753.
[14]Zhu X,Luo F,Zheng Y,et al.Characterization,purification of poncirin from edible citrus ougan(Citrus reticulate cv.suavissima)and its growth inhibitory effect on human gastric cancer cells SGC-7901[J].International journal of molecular sciences,2013,14(5):8684-8697.
[15]高伟城,蓝晓庆,潘馨.绿衣枳壳总黄酮提取工艺研究[J].天津药学,2014,26(2):38-40.
[16]滕建业,孟宪生,韩凌,等.枳壳中柚皮苷提取工艺研究[J].中国实验方剂学杂志,2011,17(3):34-35.
[17]陈海芳,魏玲,袁金斌,等.枳壳中脂溶性部位有效成分提取工艺研究[J].中成药,2010,32(9):1510-1513.
[18]易徐航,夏放高,陈海芳,等.枳壳中黄酮苷类成分对正常小鼠小肠推进作用[J].时珍国医国药,2015,26(2):278-280.
[2]蔡逸平,曹岚,范崔生.枳壳、枳实类药材的品种考证和资源应用的调查研究[J].江西中医学院学报,1998,10(4):184-186.
[3]潘馨,谢秋情,李煌诚.HPLC法同时测定绿衣枳壳中3种黄酮类化合物的含量[J].药物分析杂志,2015,35(4):666-668.
[4]李煌,张梦云.不同采收期绿衣枳壳中柚皮苷的含量测定[J].中国中医药信息杂志,2008,15(7):43-44.
[5]欧德銮,张振.枸橘药材质量标准的研究[J].海峡药学,2009,21(7):74-76.
[6]潘馨,梁鸣.绿衣枳实挥发油的GC-MS分析[J].中药新药与临床药,2004,1(6):415-416.
[7]胡源祥,谭舒舒,陈海芳,等.绿衣枳壳中葡萄内酯的含量高效液相色谱法测定[J].时珍国医国药,2016,27(8):1810-1811.
[8]毕琼瑛,施大文.枸橘质量研究[J].中药通报,1988,13(11):34-36.
[9]董晶,梁兆昌,陈海芳,等.HPLC法同时测定不同采收期江枳壳中辛弗林和N-甲基酪胺的含量[J].井冈山大学学报:自然科学版,2016,37(2):92-96.
[10]李增,陈灵芝,刘晨.枸橘苷抑制血管生成作用研究[J].时珍国医国药,2016,27(2):277-279.
[11]Jong-Bin Kim,Ah-Reum Han,Eun-Young Park,et al.inhibition of induced io NS,COX-2 and cytokines expression by poncirin through the NF-ΚB Inactivation in RAW 264.7 macrophage cells[J].Biological and Pharmaceutical Bulletin,2007,30(12):2345-2351.
[12]Dong-Hyun K I M,Eun-Ah B A E,Han M J.Anti-Helicobacter pylori activity of the metabolites of poncirin from Poncirus trifoliata by human intestinal bacteria[J].Biological and Pharmaceutical Bulletin,1999,22(4):422-424.
[13]Lee J H,Lee S H,Kim Y S,et al.Protective effects of neohesperidin and poncirin isolated from the fruits of Poncirus trifoliata on potential gastric disease[J].Phytotherapy Research,2009,23(12):1748-1753.
[14]Zhu X,Luo F,Zheng Y,et al.Characterization,purification of poncirin from edible citrus ougan(Citrus reticulate cv.suavissima)and its growth inhibitory effect on human gastric cancer cells SGC-7901[J].International journal of molecular sciences,2013,14(5):8684-8697.
[15]高伟城,蓝晓庆,潘馨.绿衣枳壳总黄酮提取工艺研究[J].天津药学,2014,26(2):38-40.
[16]滕建业,孟宪生,韩凌,等.枳壳中柚皮苷提取工艺研究[J].中国实验方剂学杂志,2011,17(3):34-35.
[17]陈海芳,魏玲,袁金斌,等.枳壳中脂溶性部位有效成分提取工艺研究[J].中成药,2010,32(9):1510-1513.
[18]易徐航,夏放高,陈海芳,等.枳壳中黄酮苷类成分对正常小鼠小肠推进作用[J].时珍国医国药,2015,26(2):278-280.