连艾煎的体外抗氧化活性研究
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摘要
目的:通过研究连艾煎的体外抗氧化作用,为充分利用连艾煎提供参考依据。方法:以黄连、艾叶质量比2∶1制备水提液;以维生素C为对照,采用分光光度法测定连艾煎清除DPPH和ABTS自由基的能力;以N-乙酰-L-半胱氨酸(NAC)作为对照,采用荧光法测定连艾煎对LPS诱导的RAW264.7巨噬细胞活性氧簇(ROS)产生的影响。结果:连艾煎清除DPPH和ABTS自由基的IC_(50)值分别为(2391.20±41.85)mg/L和(249.98±17.19)mg/L,且在一定浓度范围内存在量效关系;而连艾煎对ROS的产生具有促进作用。结论:连艾煎具有一定胞外抗氧化能力,在胞内会促进ROS的生成,为连艾煎的充分利用提供参考。
Objective To study the antioxidant effect of Lian Ai Jian in vitro, and to provide reference for full use of Lian Ai Jian. Methods Coptis chinensis and Artemisia argyi were decocted three times with a mass ratio of 2∶1. The decoction was combined and concentrated to 1 mL water solution containing 66 mg of crude drug. The DPPH and ABTS free radicals scavenging capacity of Lian Ai Jian was determined by spectrophotometry using Vitamin C as positive; the intervention ability of Lian Ai Jian on the level of reactive oxygen species(ROS) in RAW264.7 macrophages was determined by fluorescence method using N-acetyl-L-cysteine(NAC) as positive. Result The IC_(50) value of Lian Ai Jian in DPPH and ABTS free radicals scavenging was(2391.20±41.85)mg/L and(249.98±17.19)mg/L,respectively, and there was a dose-effect relationship in a certain concentration range; it was found that Lian Ai Jian promoted the ROS production in cells.Conclusion Lian Ai Jian has a certain extracellular antioxidant capacity, while it can promote the production of ROS in cells. The experimental results can provide a reference for the comprehensive utilization of Lian Ai Jian.
Objective To study the antioxidant effect of Lian Ai Jian in vitro, and to provide reference for full use of Lian Ai Jian. Methods Coptis chinensis and Artemisia argyi were decocted three times with a mass ratio of 2∶1. The decoction was combined and concentrated to 1 mL water solution containing 66 mg of crude drug. The DPPH and ABTS free radicals scavenging capacity of Lian Ai Jian was determined by spectrophotometry using Vitamin C as positive; the intervention ability of Lian Ai Jian on the level of reactive oxygen species(ROS) in RAW264.7 macrophages was determined by fluorescence method using N-acetyl-L-cysteine(NAC) as positive. Result The IC_(50) value of Lian Ai Jian in DPPH and ABTS free radicals scavenging was(2391.20±41.85)mg/L and(249.98±17.19)mg/L,respectively, and there was a dose-effect relationship in a certain concentration range; it was found that Lian Ai Jian promoted the ROS production in cells.Conclusion Lian Ai Jian has a certain extracellular antioxidant capacity, while it can promote the production of ROS in cells. The experimental results can provide a reference for the comprehensive utilization of Lian Ai Jian.
引文
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[32]Hu J,Li H,Luo X,et al.The role of oxidative stress in EBV lytic reactivation,radioresistance and the potential preventive and therapeutic implications[J].International journal of cancer,2017,141(9):1722-1729.
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[35]Davies J M S,Cillard J,Friguet B,et al.The Oxygen Paradox,the French Paradox,and age-related diseases[J].Geroscience,2017,39(5-6):499-550.
[36]Obrador E,Liu-Smith F,Dellinger R W,et al.Oxidative stress and antioxidants in the pathophysiology of malignant melanoma[J].Biological Chemistry,2018.
[37]Trachootham D,Alexandre J P.Targeting cancer cells by ROS-mediated mechanisms:a radical therapeutic approach[J].Nature Reviews Drug Discovery,2009,8(7):579-591.
[2]国家药典委员会.中华人民共和国药典(第一部)[M].北京:中国医药科技出版社,2015.
[3]张立敏,安红梅.中药复方抗氧化治疗阿尔茨海默病[J].世界中医药,2017,12(3):708-711.
[4]盖晓红,刘素香,任涛,等.黄连的化学成分及药理作用研究进展[J].中草药,2018,49(20):4919-4927.
[5]李云,王炜,尹登科,等.黄连多糖不同组分抗氧化活性比较研究[J].安徽中医药大学学报,2015,34(1):66-69.
[6]曹玲,于丹,崔磊,等.艾叶的化学成分、药理作用及产品开发研究进展[J].药物评价研究,2018,41(5):918-923.
[7]王晓琴,周成江,张娜,等.野艾蒿化学成分研究[J].中药材,2011,34(2):234-236.
[8]王惠君,王文泉,卢诚,等.艾叶研究进展概述[J].江苏农业科学,2015,43(8):15-19.
[9]胡岗,尹美珍,喻昕,等.艾叶多糖体外抗氧化作用研究[J].时珍国医国药,2015,18(11):2650-2652.
[10]Fang L,Zhang H,Zhou J,et al.Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities[J].J ANAL METHODS CHEM,2018(1):3158293.
[11]Sonklin C,Laohakunjit N,Kerdchoechuen O.Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates[J].Peerj,2018(6):e5337.
[12]阚永军,赵立,杨彬君,等.野生和林下种植金线莲的体外抗氧化活性研究[J].中国民族民间医药,2017,26(6):15-17.
[13]李生茂,刘琳,潘媛,等.川芎挥发油化学成分GC-MS分析及抗氧化活性研究[J].中国民族民间医药,2018,27(2):16-20.
[14]Lee D,Lee O H,Choi G,et al.Antioxidant and Anti-Adipogenic Activities ofTrapa japonicaShell Extract Cultivated in Korea[J].Prev.Nutr.Food Sci,2017,22(4):327-334.
[15]王吉成,刘轩,唐劲天,等.桑叶发酵前后总黄酮、总酚酸含量变化及抗氧化活性研究[J].中国民族民间医药,2014,23(23):15-18.
[16]侯丽丽,陈婷,刘礼婷,等.生长激素释放多肽对高糖诱导视网膜色素上皮细胞的保护作用[J].国际眼科杂志,2018,18(7):1184-1187.
[17]谢海洲,卢祥之,谢芳.中医历代良方全书[M].青岛:青岛出版社,2006:482.
[18]付烊,张艳,运强.放射性肠炎的中医药治疗进展[J].中医学报,2016,31(1):121-124.
[19]Conversely.Nutrients and Oxidative Stress:Friend or Foe[J].OXID MED CELL LONGEV,2018,2018(6):314-321.
[20]Liguori I,Russo G,Curcio F,et al.Oxidative stress,aging,and diseases[J].Clin Interv Aging,2018,13(1):757-772.
[21]Gorrini C,Harris I S,Mak T W.Modulation of oxidative stress as an anticancer strategy[J].Nature reviews Drug discovery,2013,12(12):931.
[22]Helfinger V,Von Gall F F,Henke N,et al.Hydrogen peroxide formation by Nox4 limits malignant transformation[J].bioRxiv,2017:177055.
[23]Helfinger V,Schroeder K.Redox control in cancer development and progression[J].Molecular aspects of medicine,2018.
[24]Govindarajan B,Sligh J E,Vincent B J,et al.Overexpression of Akt converts radial growth melanoma to vertical growth melanoma[J].The Journal of clinical investigation,2007,117(3):719-729.
[25]Liou GY,Storz P.Reactive oxygen species in cancer[J].Free radical research,2010,44(5):479-496.
[26]Wang J,Yi J.Cancer cell killing via ROS:to increase or decrease,that is the question[J].Cancer biology & therapy,2008,7(12):1875-1884.
[27]Seifried H E,Mcdonald S S,Anderson D E,et al.The antioxidant conundrum in cancer[J].Cancer research,2003,63(15):4295-4298.
[28]Group A-T B C C P S.The effect of vitamin E and beta carotene on the incidence of lung cancer and other cancers in male smokers[J].New England Journal of Medicine,1994,330(15):1029-1035.
[29]Sayin V I,Ibrahim M X,Erik L,et al.Antioxidants accelerate lung cancer progression in mice[J].SCI TRANSL MED,2014,6(221):1-30.
[30]Lee S Y,Jeong E K,Ju M K,et al.Induction of metastasis,cancer stem cell phenotype,and oncogenic metabolism in cancer cells by ionizing radiation[J].Molecular cancer,2017,16(1):10.
[31]Zhen X,Cheng P,Pu K.Recent Advances in Cell Membrane–Camouflaged Nanoparticles for Cancer Phototherapy[J].Small,2018:1804105.
[32]Hu J,Li H,Luo X,et al.The role of oxidative stress in EBV lytic reactivation,radioresistance and the potential preventive and therapeutic implications[J].International journal of cancer,2017,141(9):1722-1729.
[33]Chandel N S,Tuveson D A.The Promise and Perils of Antioxidants for Cancer Patients — NEJM[J].N Engl J Med,2014,371(2):177-178.
[34]Watson J.Oxidants,antioxidants and the current incurability of metastatic cancers[J].OPEN BIOL,2013,3(120144):1-9.
[35]Davies J M S,Cillard J,Friguet B,et al.The Oxygen Paradox,the French Paradox,and age-related diseases[J].Geroscience,2017,39(5-6):499-550.
[36]Obrador E,Liu-Smith F,Dellinger R W,et al.Oxidative stress and antioxidants in the pathophysiology of malignant melanoma[J].Biological Chemistry,2018.
[37]Trachootham D,Alexandre J P.Targeting cancer cells by ROS-mediated mechanisms:a radical therapeutic approach[J].Nature Reviews Drug Discovery,2009,8(7):579-591.