油橄榄果渣中多酚的提取与纯化研究
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摘要
以油橄榄果渣为原料,利用微波辅助技术提取油橄榄果渣多酚并对其进行纯化研究。通过单因素和正交试验优化橄榄多酚提取工艺,最佳优化工艺参数为液料比20 mL/g、微波时间2 min、微波功率200 W、乙醇体积分数50%。在此条件下,油橄榄果渣多酚得率为0.97%。对比5种大孔树脂对油橄榄酚类粗提物的纯化效果可知,经AB-8型树脂纯化后油橄榄果渣多酚含量可提高至80%。
With olive pomace as raw material, the olive pomace polyphenols was extracted and purified by microwave–assisted technology. Single factor method and orthogonal array design were used to optimize the extraction process of olive polyphenols. The optimum parameters were as follows: liquid-solid ratio 20 mL/g, microwave time 2 min, microwave power 200 W and ethanol concentration 50%. Under these conditions, the yield of polyphenol in olive pomace was 0.97%. Comparing the purification effect of 5 kinds of macroporous resin on the crude extraction of olive phenol, the content of olive pomace polyphenol increased to 80% after the purification of AB-8 macroporous resin.
With olive pomace as raw material, the olive pomace polyphenols was extracted and purified by microwave–assisted technology. Single factor method and orthogonal array design were used to optimize the extraction process of olive polyphenols. The optimum parameters were as follows: liquid-solid ratio 20 mL/g, microwave time 2 min, microwave power 200 W and ethanol concentration 50%. Under these conditions, the yield of polyphenol in olive pomace was 0.97%. Comparing the purification effect of 5 kinds of macroporous resin on the crude extraction of olive phenol, the content of olive pomace polyphenol increased to 80% after the purification of AB-8 macroporous resin.
引文
[1]王成章,高彩霞,姜成英.油橄榄的化学组成和加工利用[J].林业科技开发,2006,20(2):1-4.
[2]ZARZUELO A,DUARTE J,JIMENEZ J,et al.Vasodilator effect of olive leaf[J].Planta Medica,1991,57(5):417-419.
[3]夏雅俊,刘永峰,裴栋,等.中压制备色谱法分离制备油橄榄叶中多酚类化合物[J].中草药,2014,45(12):1689-1692.
[4]陈岗.橄榄中多酚类物质的分离纯化及功能性研究[D].重庆:西南大学,2009.
[5]林玉芳,陈清西,关夏玉,等.橄榄总多酚提取工艺优化研究[J].中国农学通报,2011,7(5):396-400.
[6]孔维宝,李阳,白万明,等.微波辅助提取油橄榄果渣多酚[J].食品与发酵工业,2011,37(4):233-237.
[7]何志勇,夏文水.Folin-Ciocalteu比色法测定橄榄中多酚含量的研究[J].林产化学与工业,2006,26(4):15-18.
[8]梁瑞红,袁敏,李倩,等.大孔吸附树脂分离纯化南酸枣枣皮多酚的研究[J].南昌大学学报(工科版),2016,38(1):1-6.
[9]何志勇.橄榄酚类化合物的分离纯化和结构研究[D].无锡:江南大学,2007.
[10]马乐,韩军岐,白欢欢,等.采用大孔树脂分离纯化核桃青皮中多酚[J].食品与发酵工业,2016,42(3):237-242.
[11]王晓群.微波辅助提取猕猴桃叶多酚及其抗氧化性的研究[J].粮食与油脂,2016,29(3):77-80.
[12]李健,安廷,刘宁,等.微波辅助响应曲面法优化提取苹果多酚工艺[J].食品安全质量检测学报,2015(6):2029-2035.
[13]何志勇.橄榄酚类化合物的分离纯化和结构研究[D].无锡:江南大学,2007.
[2]ZARZUELO A,DUARTE J,JIMENEZ J,et al.Vasodilator effect of olive leaf[J].Planta Medica,1991,57(5):417-419.
[3]夏雅俊,刘永峰,裴栋,等.中压制备色谱法分离制备油橄榄叶中多酚类化合物[J].中草药,2014,45(12):1689-1692.
[4]陈岗.橄榄中多酚类物质的分离纯化及功能性研究[D].重庆:西南大学,2009.
[5]林玉芳,陈清西,关夏玉,等.橄榄总多酚提取工艺优化研究[J].中国农学通报,2011,7(5):396-400.
[6]孔维宝,李阳,白万明,等.微波辅助提取油橄榄果渣多酚[J].食品与发酵工业,2011,37(4):233-237.
[7]何志勇,夏文水.Folin-Ciocalteu比色法测定橄榄中多酚含量的研究[J].林产化学与工业,2006,26(4):15-18.
[8]梁瑞红,袁敏,李倩,等.大孔吸附树脂分离纯化南酸枣枣皮多酚的研究[J].南昌大学学报(工科版),2016,38(1):1-6.
[9]何志勇.橄榄酚类化合物的分离纯化和结构研究[D].无锡:江南大学,2007.
[10]马乐,韩军岐,白欢欢,等.采用大孔树脂分离纯化核桃青皮中多酚[J].食品与发酵工业,2016,42(3):237-242.
[11]王晓群.微波辅助提取猕猴桃叶多酚及其抗氧化性的研究[J].粮食与油脂,2016,29(3):77-80.
[12]李健,安廷,刘宁,等.微波辅助响应曲面法优化提取苹果多酚工艺[J].食品安全质量检测学报,2015(6):2029-2035.
[13]何志勇.橄榄酚类化合物的分离纯化和结构研究[D].无锡:江南大学,2007.