BMSCs移植对AD大鼠海马组织SOD、AchE活性与MDA、Ach含量的影响及补脑Ⅰ号的干预作用
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摘要
目的:BMSCs移植对AD大鼠海马组织SOD、AchE活性与MDA、Ach含量的影响及补脑Ⅰ号的干预作用。方法:将80只SD大鼠,随机分为4组,每组20只,分别为:模型组、假手术组、BMSCs移植组(移植组)、中药+BMSCs移植组(中药+移植组),造模方法釆用连续6周三氯化铝溶液灌胃及D-半乳糖溶液腹注射,动物造模7天后予BMSCs移植,移植后2h补脑Ⅰ号浓缩药物灌胃,连续4周,检测大鼠海马组织SOD、AchE活性与MDA、Ach含量。结果:模型组与移植组、中药+移植组相比较,大鼠海马组织SOD、AchE活性与MDA、Ach含量检测均显示出显著差异(P<0. 01)。结论:BMSCs移植可提高AD大鼠自由基代谢及机体抗氧化的能力;有降低乙酰胆碱酯酶活性,减少乙酰胆碱水解的作用,且补脑Ⅰ号起到增强作用。
Objective: To study the effect of BMSCs on the activity of SOD and AchE and the content of MDA and Ach in the hippocampus of AD rats as well as the intervention effect of chinese medicine( Bu Nao I Hao). Methods: Eighty SD rats were randomly divided into 4 groups,20 rats in each group,they are model group,sham operation group,BMSCs transplantation group,Chinese medicine + BMSCs transplantation group.The alumina solution and D-galactose solution femoral injection were used to model group for 6 weeks. After seven days,BMSCs transplantation was used for animal model group. After transplantation for 2 hours,the activity of SOD and AchE as well as the contents of MDA and Ach in the hippocampus of rats were detected for 4weeks. Results: The activity of SOD and AchE in hippocampus of model group was compared with that of BMSCs transplantation group and traditional Chinese medicine + BMSCs transplantation group,the Ach content detection showed significant difference( P<0. 01).Conclusions: BMSCs transplantation can improve the free radical metabolism and anti-oxidation ability of AD rats; it can decrease the activity of acetylcholinesterase and decrease the hydrolysis of acetyl gallate,and enhance the function of chinese medicine( Bu Nao I Hao).
Objective: To study the effect of BMSCs on the activity of SOD and AchE and the content of MDA and Ach in the hippocampus of AD rats as well as the intervention effect of chinese medicine( Bu Nao I Hao). Methods: Eighty SD rats were randomly divided into 4 groups,20 rats in each group,they are model group,sham operation group,BMSCs transplantation group,Chinese medicine + BMSCs transplantation group.The alumina solution and D-galactose solution femoral injection were used to model group for 6 weeks. After seven days,BMSCs transplantation was used for animal model group. After transplantation for 2 hours,the activity of SOD and AchE as well as the contents of MDA and Ach in the hippocampus of rats were detected for 4weeks. Results: The activity of SOD and AchE in hippocampus of model group was compared with that of BMSCs transplantation group and traditional Chinese medicine + BMSCs transplantation group,the Ach content detection showed significant difference( P<0. 01).Conclusions: BMSCs transplantation can improve the free radical metabolism and anti-oxidation ability of AD rats; it can decrease the activity of acetylcholinesterase and decrease the hydrolysis of acetyl gallate,and enhance the function of chinese medicine( Bu Nao I Hao).
引文
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[2]贾桃. MSCs移植对AD大鼠海马突触素蛋白表达的影响及补脑Ⅰ号干预作用[D].贵阳:贵阳中医学院,2014.
[3]叶民.阿尔茨海默病病因及发病机制研究进展[J].实用老年医学,2017,31(05):402-406.
[4]田由,王雪,艾静.基底前脑胆碱能神经元功能异常在阿尔茨海默病中的作用相关研究进展[J].神经药理学报,2017,7(04):53-64.
[5]常富业,单媛莉,张云岭,等.醒脑液对双转基因痴呆小鼠行为学及血浆中胆碱能系统相关酶的影响[J].中华中医药学刊,2014,32(03):460-462.
[6] Bajda M,Cuzior N,Ignasik M,et al. Multi-target-directed ligands in Alzheimer’s disease treatment[J].Curent Medicinal Chemistry,2011,18(32):4949-4975.
[7]孙治坤,马兴荣,韩笑,等.白藜芦醇对阿尔茨海默病合并糖尿病大鼠的氧化应激作用[J].中国实用神经疾病杂志,2017,20(23):1-5.
[8]王春花,刘克明,张明月,等. SOD基因表达与衰老的相关性[J].中国公共卫生,2004,20(8):953-954.
[9]黎瑞珍,杨庆建,陈贻锐.超氧化物歧化酶(SOD)活性的测定及其应用研究[J].琼州大学学报,2004,11(5):34-36.
[10] Fukunaga,Deane R,Bell RD,et al. Clearane of amyloid beta by circulating lipoprotein receptor[J]. Nat Med,2007:13(9):1029-1031.
[11]况时祥,杨辉,刘琛,等.补脑Ⅰ号治疗老年人轻度认知功能障碍临床观察[J].新中医,2011,43(8):31-32.