羧基化碳纳米片电化学传感器的研制及其在喹赛多灵敏检测中的应用
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摘要
本文构筑了一种基于羧基化碳纳米片修饰电极的喹赛多电化学传感器,该传感器对喹赛多的电化学还原具有优良的催化性能。与金电极相比,其电流增幅达到30余倍。通过优化羧基化碳纳米片涂覆量,Na_3PO_4浓度,电解液pH,富集电位,富集时间,搅拌速度等测试条件,建立了基于羧基化碳纳米片修饰电极的喹赛多超高灵敏度分析方法,其检出限可达3.79×10~(-9) mol·L~(-1)(S/N=3)。利用该方法对加标猪肉样品进行检测,结果令人满意。
In this study,a simple electrochemical sensor(ECS)was built based on carboxylated carbon nanosheets modified electrode.The ECS showed good electro-catalytic performance on the reduction of cyadox.The current of modified electrode were increased by more than 30 times compared with that of the gold electrode.A series of experimental conditions were systematically optimized.The modified electrode currents were linear to the concentration of cyadox from the range of 2.0×10~(-8) to 2.0×10~(-6) mol·L~(-1) with a detection limit of 3.79×10~(-9 )mol·L~(-1)(S/N=3).The ECS was successfully applied for determination of cyadox in real samples.
In this study,a simple electrochemical sensor(ECS)was built based on carboxylated carbon nanosheets modified electrode.The ECS showed good electro-catalytic performance on the reduction of cyadox.The current of modified electrode were increased by more than 30 times compared with that of the gold electrode.A series of experimental conditions were systematically optimized.The modified electrode currents were linear to the concentration of cyadox from the range of 2.0×10~(-8) to 2.0×10~(-6) mol·L~(-1) with a detection limit of 3.79×10~(-9 )mol·L~(-1)(S/N=3).The ECS was successfully applied for determination of cyadox in real samples.
引文
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[2]郭霞,孙建华,孙振中,等.水产品中喹烯酮和喹赛多及其主要代谢物残留的HPLC-MS/MS检测方法研究[J].分析测试学报,2016,35(12):1535-1541.
[3]Alagawany M,Farag M R,Abd El-Hack M E,et al.Assessment of cyadox effects on the antioxidant defense system and hemolysis of isolated rabbit erythrocytes[J].Int J Pharmacol,2017,13:183-190.
[4]Hao H H,Guo W G,Iqbal Z,et al.Impact of cyadox on human colonic microflora in chemostat modelsle[J].Regul Toxicol Pharm,2013,67:335-343.
[5]Huang Q,Ihsan A,Guo P,et al.Evaluation of the safety of primary metabolites of cyadox:Acute and sub-chronic toxicology studies and genotoxicity assessment[J].Regul Toxicol Pharm,2016,74:123-136.
[6]Ihsan A,Wang X,Zhang W,et al.Genotoxicity of quinocetone,cyadox and olaquindox in vitro and in vivo[J].Food Chem Toxicol,2013,59:207-214.
[7]Wang X,Zhou W,Ihsan A,et al.Assessment of thirteen-week subchronic oral toxicity of cyadox in Beagle dogs[J].Regul Toxicol Pharm,2015,73:652-659.
[8]Wang X,Fang G J,Wang Y L,et al.Two generation reproduction and teratogenicity studies of feeding cyadox in Wistar rats[J].Food Chem Toxicol,2011,49:1068-1079.
[9]Huang L L,Wang Y L,Tao Y F,et al.Development of high performance liquid chromatographic methods for the determination of cyadox and its metabolites in plasma and tissues of chicken[J].J Chromatogr B,2008,874:7-14.
[10]Zhang Y L,Huang L L,Chen D M,et al.Development of HPLC methods for the determination of cyadox and its main metabolites in goat tissues[J].Anal Sci,2005,21:1495-1499.
[11]He L M,Liu K Y,Su Y J,et al.Simultaneous determination of cyadox and its metabolites in plasma by high-performance liquid chromatography tandem mass spectrometry[J].J Sep Sci,2011,34:1755-1762.
[12]Cheng L L,Wang Z H,Shen J Z,et al.A specific UPLC-ESI-MS/MS method for analysis of cyadox and its three main metabolites in fish samples[J].Anal Method,2012,4:217-221.
[13]Miao X H,Xu L J,Li H P,et al.Determination of olaquindox,carbadox and cyadox in animal feeds by ultra-performance liquid chromatography tandem mass spectrometry[J].Food Addit Contam A,2018,35:1257-1265.
[14]Yan D D,He L M,Zhang G J,et al.Simultaneous determination of cyadox and its metabolites in chicken tissues by LC-MS/MS[J].Food Anal Method,2012,5:1497-1505.
[15]赵春保,彭大鹏,陶燕飞,等.猪饲料中喹噁啉类药物酶联免疫检测方法的建立[J].中国农业科技导报,2008,10(S2):69-75.
[16]Zhu C Z,Yang G H,Li H,et al.Electrochemical sensors and biosensors based on nanomaterials and nanostructures[J].Anal Chem,2015,87:230-249.
[17]Yanez-Sedeno P,Campuzano S,Pingarron J M.Electrochemical sensors based on magnetic molecularly imprinted polymers:A review[J].Anal Chim Acta,2017,960:1-17.
[18]Wang H,Yao S,Liu Y,et al.Molecularly imprinted electrochemical sensor based on Au nanoparticles in carboxylated multi-walled carbon nanotubes for sensitive determination of olaquindox in food and feedstuffs[J].Biosen Bioelectron,2017,87(1):417-421.
[19]Ding J,Zhou H,Zhang H L,et al.Exceptional energy and new insight with a sodium-selenium battery based on a carbon nanosheet cathode and a pseudographite anode[J].Energ Environ Sci,2017,10:153-165.
[20]Song Y F,Yang J,Wang K,et al.In-situ synthesis of graphene/nitrogen-doped ordered mesoporous carbon nanosheet for supercapacitor application[J].Carbon,2016,96:955-964.
[21]Zhou J W,Qin J,Zhang X,et al.2D space-confined synthesis of few-layer MoS2 anchored on carbon nanosheet for lithium-ion battery anode[J].ACS Nano,2015,9:3837-3848.
[22]Ding J,Wang H L,Li Z,et al.Carbon nanosheet frameworks derived from peat moss as high performance sodium ion battery anodes[J].ACS Nano,2013,7:11004-11015.
[23]De Volder M F L,Tawfick S H,Baughman RH,et al.Carbon nanotubes:Present and future commercial applications[J].Science,2013,339:535-539.
[24]Hecht D S,Hu L B,Irvin G.Emerging transparent electrodes based on thin films of carbon nanotubes,graphene,and metallic nanostructures[J].Adv Mater,2011,23:1482-1513.
[25]Liu Y X,Dong X C,Chen P.Biological and chemical sensors based on graphene materials[J].Chem Soc Rev,2012,41:2283-2307.
[26]Justino C I L,Comes A R,Freitas A C,et al.Graphene based sensors and biosensors[J].TRAC-Trend Anal Chem,2017,91:53-66.
[27]Yao S,Li G K,Liu Y Q,et al.Electrochemical detection of pyridoxal using a sonoelectrochemical prepared highly-oxidized carbon nanosheets modified electrode[J].Sensor Actuat B Chem,2018,274:324-330.