Axin1和Axin2基因在急性白血病中的表达及临床意义
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摘要
目的:探讨Axin1和Axin2基因的表达与急性白血病发生发展的关系及其临床意义。方法:通过实时定量PCR技术检测36例急性白血病患者及15例对照组骨髓单个核细胞中Axin1和Axin2 mRNA的表达情况。结果:Axin1在初诊组、缓解组和未缓解组中的表达量均低于对照组(P<0.05),初诊组与缓解组、初诊组与未缓解组的表达量均无明显差异(P>0.05),缓解组与未缓解组初诊时Axin1的表达无明显差异(P>0.05);Axin2在缓解组中的表达量比对照组明显减低(P<0.05),未缓解组比缓解组初诊时Axin2的表达明显增高(P<0.05),初诊组与对照组、初诊组与未缓解组的表达均无明显差异(P>0.05)。结论:Axin1与急性白血病的发生诊断相关,Axin2则可以作为急性白血病的一个预后指标。
Objective:To investigate the roles of Axin1 and Axin2 genes when acute leukemia occurred and developed. Methods:Axin1 and Axin2 mRNA of 36 cases of acute leukemia patients and 15 normal bone marrow donors were detected by real-time quantitative PCR. Results:Aixn1 was lower in newly diagnosed group,remission group and non-remission group than the control group(P < 0. 05). There were no significant differences compared the newly diagnosed group and the remission group,and the non-remission group(P > 0. 05). Expression of Axin1 in the non-remission group was similar to the remission group when newly diagnosed(P > 0. 05). Axin2 was significantly lower in the remission group than the newly diagnosed group(P < 0. 05). Expression of Axin2 in the non-remission group was higher than the remission group when newly diagnosed(P < 0. 05). Compared the newly diagnosed group and the control group,the newly diagnosed group and the non-remission group,the results had high similarity(P >0. 05). Conclusion:Axin1 is associated with the occurrence of acute leukemia. Axin2 may be as a prognostic indicator in acute leukemia.
Objective:To investigate the roles of Axin1 and Axin2 genes when acute leukemia occurred and developed. Methods:Axin1 and Axin2 mRNA of 36 cases of acute leukemia patients and 15 normal bone marrow donors were detected by real-time quantitative PCR. Results:Aixn1 was lower in newly diagnosed group,remission group and non-remission group than the control group(P < 0. 05). There were no significant differences compared the newly diagnosed group and the remission group,and the non-remission group(P > 0. 05). Expression of Axin1 in the non-remission group was similar to the remission group when newly diagnosed(P > 0. 05). Axin2 was significantly lower in the remission group than the newly diagnosed group(P < 0. 05). Expression of Axin2 in the non-remission group was higher than the remission group when newly diagnosed(P < 0. 05). Compared the newly diagnosed group and the control group,the newly diagnosed group and the non-remission group,the results had high similarity(P >0. 05). Conclusion:Axin1 is associated with the occurrence of acute leukemia. Axin2 may be as a prognostic indicator in acute leukemia.
引文
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[3]Seiji Satoh,Yataro Daigo,Yoichi Furukawa,et al.Axin1 mutations in hepatocellular carcinomas,and growth suppression in cancer cells by virus-mediated transfer of Axin1[J].Nature Genetics,2000,24:245-250.
[4]Xu HT,Wang L,Lin D,et al.Abnormalβ-catenin and reduced Axin expression are associated with poor differentiation and progression in non-small cell lung cancer[J].Am J Clin Pathol,2006,125(4):534-541.
[5]Taipale J,Beachy PA.The hedgehog and Wnt signaling pathways in cancer[J].Nature,2001,411(6835):349-354.
[6]Jamieson CHM,Ailles LE,Jones C,et al.The role of beta-catenin in chronic myelogenous leukemic progenitor expansion[J].Blood,2003,102:510-512.
[7]Chen SM,Sun H,Liu YF,et al.Expression and role ofβ-catenin in acute leukemia bone marrow cells[J].J Experimental Hematol,2006,14(3):460-463.[陈胜梅,孙慧,刘延方,等.β-连环蛋白在急性白血病骨髓细胞中的表达及其意义[J].中国实验血液学杂志,2006,14(3):460-463.]
[8]Feng GJ,Welwyn Cotta,Wei XQ,et al.Conditional disruption of Axin1 leads to development of liver tumors in mice[J].Gastroenterology,2012,143:1650-1659.
[9]Nicola E,Potter,Mel Greaves.Persistence of leukaemic ancestors[J].Nature,2014,506:300.
[10]Li S,Wang CP,Liu XD,et al.The roles of Axin2 in tumorigenesis and epigenetic regulation[J].Familial Cancer,2015,14:325-331.
[11]Wu ZT,Sun YF,Xu H,et al.Axin2 rs2240308 polymorphism contributes to increased cancer risk:evidence based on a meta-analysis[J].Cancer Cell International,2015,15:68.
[12]Brian R Hu,Adrian S Fairey,Anisha Madhav,et al.Axin2 expression predicts prostate cancer recurrence and regulates invasion and tumor growth[J].The Prostate,2016,76:597-608.
[13]Zhao QW,Thomas B,Eric Fearona,et al.Canonical Wnt suppressor,Axin2,promotes colon carcinoma oncogenic activity[J].PNAS,2012,109(28):11312-11317.
[14]N Figeac,PS Zammit.Coordinated action of Axin1 and Axin2 suppressesβ-catenin to regulate muscle stem cell function[J].Cellular Signalling,2015,27:1652-1665.