当归茎叶与根对小鼠溶血性贫血模型的影响
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摘要
目的探讨当归茎叶与根对溶血性贫血小鼠的干预作用。方法将90只小鼠随机分为9组:空白组,模型组,阳性组,当归茎叶水煎液组(高、中、低剂量),当归根水煎液组(高、中、低剂量),每组10只,♀♂各半。采用乙酰苯肼(acetyl phenyl hydrazine,APH)制造溶血性贫血模型(1,4,7 d皮下注射含有APH的生理盐水,APH剂量为0.2,0.1,0.1 g·kg~(-1)),造模第1天给予当归根水煎液与茎叶水煎液灌胃治疗(0.02 mL·g~(-1)),13 d后取样,检测其血常规(Hb、RBC、PLT)、脏器指数(脾、胸腺指数)、生化指标(G6PD、GR、IL-2和EPO)以及观察肝、脾病理切片(苏木精-伊红染色)。结果与模型组比较,当归茎叶水煎液与当归根水煎液均能显著升高Hb、RBC、PLT,降低脾指数,提高胸腺指数,升高小鼠血液中G6PD、GR、IL-2、EPO的含量(P<0.05或<0.01)。另外,病理切片结果显示,当归茎叶水煎液和当归根水煎液均可减轻APH所致小鼠肝脏、脾脏细胞的点状坏死及瘀血。结论当归茎叶水煎液与当归根水煎液对溶血性贫血小鼠均具有一定的改善作用。
OBJECTIVE To study the effect of Angelica sinensis stems and leaves and Angelica sinensis roots on hemolytic anemia model mice. METHODS Ninety mice were randomly divided into 9 groups: control group, model group, positive group, and Angelica sinensis stems and leaves(high, middle and low dose) and Angelica sinensis roots(high, middle and low dose) groups, 10 mice in each group, half male and half female. The animal model of hemolytic anemia was made by using acetyl phenyl hydrazine(APH)(1, 4, 7 d hypodermic inject saline containing 0.2, 0.1, 0.1 g·kg~(-1) APH, respectively). On the first day of modeling, Angelica sinensis roots decoction, Angelica sinensis stems and leaves decoction were given to mice by gavage(0.02 mL·g~(-1)). Samples were taken 13 d later to test their blood routine(Hb, RBC, PLT), organ index(spleen index, thymus index), biochemical indicators(G6PD, GR, IL-2 and EPO), and to observe pathological sections of liver and spleen with HE staining. RESULTS Compared with the model group, Angelica sinensis stems and leaves, roots decoction could significantly increase Hb, RBC, PLT, decrease spleen index and improve the thymus index. Besides, increased the levels of G6PD, GR, IL-2 and EPO in the blood of mice(P<0.05 or <0.01). In addition, the results of pathological sections showed that Angelica sinensis stems and leaves decoction, Angelica sinensis roots decoction both reduced the point necrosis and congestion of liver and spleen cells in mouse induced by APH. CONCLUSION The Angelica sinensis stems and leaves, and Angelica sinensis roots have improving effect on hemolytic anemia mice.
OBJECTIVE To study the effect of Angelica sinensis stems and leaves and Angelica sinensis roots on hemolytic anemia model mice. METHODS Ninety mice were randomly divided into 9 groups: control group, model group, positive group, and Angelica sinensis stems and leaves(high, middle and low dose) and Angelica sinensis roots(high, middle and low dose) groups, 10 mice in each group, half male and half female. The animal model of hemolytic anemia was made by using acetyl phenyl hydrazine(APH)(1, 4, 7 d hypodermic inject saline containing 0.2, 0.1, 0.1 g·kg~(-1) APH, respectively). On the first day of modeling, Angelica sinensis roots decoction, Angelica sinensis stems and leaves decoction were given to mice by gavage(0.02 mL·g~(-1)). Samples were taken 13 d later to test their blood routine(Hb, RBC, PLT), organ index(spleen index, thymus index), biochemical indicators(G6PD, GR, IL-2 and EPO), and to observe pathological sections of liver and spleen with HE staining. RESULTS Compared with the model group, Angelica sinensis stems and leaves, roots decoction could significantly increase Hb, RBC, PLT, decrease spleen index and improve the thymus index. Besides, increased the levels of G6PD, GR, IL-2 and EPO in the blood of mice(P<0.05 or <0.01). In addition, the results of pathological sections showed that Angelica sinensis stems and leaves decoction, Angelica sinensis roots decoction both reduced the point necrosis and congestion of liver and spleen cells in mouse induced by APH. CONCLUSION The Angelica sinensis stems and leaves, and Angelica sinensis roots have improving effect on hemolytic anemia mice.
引文
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[15]张文卓.参红补血颗粒对血虚动物模型造血及免疫功能影响的作用机制研究[D].长春:长春中医药大学,2014.
[16]HUANG L P,WU S F,ZHOU J,et al.Effect comparison of Angelicae sinensis decoction for supplementing blood on three models of hematopenia mouse[J].Pharm Clin Chin Mater Med(中药药理与临床),2011(5):5-8.
[17]廖世初.胶芪养血颗粒对动物血虚证模型的主要药效学研究[D].北京:北京中医药大学,2010.
[18]孙红国.基于代谢组学的当归补血作用研究[D].兰州:甘肃农业大学,2013.
[19]WANG R Y,XI J F,HE L J,et al.Establishment of an acute hemolytic anemia mouse model induced by phenylhydrazine and its potential applications in regenerative medicine[J].Lett Biotech(生物技术通讯),2010,21(4):544-546.
[20]杨应文,王亚丽,潘新波,等.当归不同生长部位1H-NMR指纹图谱的研究[C].甘肃省化学会第二十八届年会暨第十届中学化学教学经验交流会论文集.兰州:2013:3.
[21]杨应文,王亚丽,潘新波,等.当归不同生长部位HPLC指纹图谱的研究[C].西北地区第七届色谱学术报告会暨甘肃省第十二届色谱年会论文集.兰州:2012:43-44.
[2]GU Z R,WANG Y L,SUN Y J,et al.Simultaneous determination of five constituents in Angelica sinensis from different areas and the quality evaluation[J].Chin Tradit Pat Med(中成药),2014,36(10):2135-2140.
[3]梁飞.道地药材考-以20种中药为例[D].北京:北京中医药大学,2013:128-129.
[4]李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,36(6):1023-1028.
[5]JIN M,ZHAO K,HUANG Q,et al.Isolation,structure and bioactivities of the polysaccharides from Angelica sinensis(Oliv.)Diels:a review[J].Carbohydr Polym,2012,89(3):713-722.
[6]HOU H F,BAO Y Z,LI Q,et al.Preparation of blood-deficient model and research of angelica polysaccharide on enriching blood in chickens[J].Evid Based Complement Alternat Med,2012,2012(1):965947.
[7]XIAO H,XIONG L,SONG X,et al.Angelica sinensis polysaccharides ameliorate stress-induced premature senescence of hematopoietic cell via protecting bone marrow stromal cells from oxidative injuries caused by 5-fluorouracil[J].Int J Mol Sci,2017,18(11):2265.
[8]LUO H Y,HUANG Y H.Protective effect of Angelicae Sinensis Radix volatile oil on acute myocardial ischemic injury in mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(2):159-162.
[9]张保国,刘庆芳.当归四逆加吴茱萸生姜汤药理研究与临床应用[J].中成药,2013,35(8):1745-1747.
[10]ZHANG B B,DIAO T T,DAI M Z,et al.Beauty pharmacology of activating blood circulation herbs and its application in modern cosmetics as plant extracts[J].Chin JMod Appl Pharm(中国现代应用药学),2016,33(9):1221-1226.
[11]萨日娜,潘新波,王亚丽,等.当归不同生长部位挥发油的GC-MS指纹图谱研究[C].海峡两岸暨CSNR全国第10届中药及天然药物资源学术研讨会论文集.兰州:2013:474-477.
[12]HUANG B,LI J,WANG X Z,et al.Establishment of hemolytic anemia rat model and its laboratory evaluation[J].JChongqing Med Univ(重庆医科大学学报),2010,35(5):703-705.
[13]纪鹏.生当归及其不同炮制品干预小鼠血虚模型的代谢组学研究[D].兰州:甘肃农业大学,2015.
[14]宋志学.当归水煎液对血虚小鼠补血作用的差异蛋白组学研究[D].兰州:甘肃农业大学,2013.
[15]张文卓.参红补血颗粒对血虚动物模型造血及免疫功能影响的作用机制研究[D].长春:长春中医药大学,2014.
[16]HUANG L P,WU S F,ZHOU J,et al.Effect comparison of Angelicae sinensis decoction for supplementing blood on three models of hematopenia mouse[J].Pharm Clin Chin Mater Med(中药药理与临床),2011(5):5-8.
[17]廖世初.胶芪养血颗粒对动物血虚证模型的主要药效学研究[D].北京:北京中医药大学,2010.
[18]孙红国.基于代谢组学的当归补血作用研究[D].兰州:甘肃农业大学,2013.
[19]WANG R Y,XI J F,HE L J,et al.Establishment of an acute hemolytic anemia mouse model induced by phenylhydrazine and its potential applications in regenerative medicine[J].Lett Biotech(生物技术通讯),2010,21(4):544-546.
[20]杨应文,王亚丽,潘新波,等.当归不同生长部位1H-NMR指纹图谱的研究[C].甘肃省化学会第二十八届年会暨第十届中学化学教学经验交流会论文集.兰州:2013:3.
[21]杨应文,王亚丽,潘新波,等.当归不同生长部位HPLC指纹图谱的研究[C].西北地区第七届色谱学术报告会暨甘肃省第十二届色谱年会论文集.兰州:2012:43-44.