新生儿缺氧缺血性脑病患儿外周血单核细胞CD14、TLR4及TRIF的表达水平及意义
摘要
目的探讨新生儿缺氧缺血性脑病(HIE)患儿外周血单核细胞CD14、TLR4和TRIF表达水平与HIE严重程度和预后的相关性。方法选取2017年1-10月在中国人民解放军第101医院住院的HIE患儿36例为HIE组,另选取同期在该院体检的健康新生儿23例为对照组,采用实时荧光定量PCR方法检测两组新生儿外周血单核细胞CD14、TLR4和TRIF mRNA的表达水平,分析CD14、TLR4和TRIF表达差异及与HIE严重程度和预后的相关性。结果 HIE患儿外周血单核细胞CD14、TLR4和TRIF水平高于对照组,差异有统计学意义(P<0. 05)。CD14、TLR4和TRIF与HIE严重程度呈正相关关系(P<0. 05),与预后效果呈负相关关系(P<0. 05)。结论 CD14,TLR4和TRIF表达水平与HIE的发生发展有关。
引文
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[13]Bauer S,Hartmann G.Toll-Like Receptors(TLRs)and Innate Immunity[J].Handb Exp Pharmacol,2008,183(5):267-276.
[14]梁桂娟,王迎涛,唐成和,等.Toll样受体4在新生大鼠缺氧缺血性脑损伤中的表达变化[J].中国新生儿科杂志,2016,31(3):222-225.
[15]黄晓雷,崔桂云.新生小鼠缺血缺氧性脑损伤中TLR4-TRIF信号通路的表达[J].中国实用神经疾病杂志,2012,15(9):16-18.
[16]喻婷,李晓瑜,邹晓萍,等.米诺环素对缺氧缺血脑损伤未成熟新生大鼠TLR4、NF-κBp65和TNF-α表达的影响[J].解剖学研究,2013(6):401-408.
[2]Kumar K.Hypoxic-ischemic brain damage in perinatal age group[J].Indian J Pediatr,1999,66(4):475-482.
[3]Zhou M,Wang CM,Yang WL,et al.Microglial CD14 Activated by i NOS Contributes to Neuroinflammation in Cerebral Ischemia[J].Brain Res,2013,1506(2):105-114.
[4]Nikodemova M,Watters JJ.Outbred ICR/CD1 mice display more severe neuroinflammation mediated by microglial TLR4/CD14 activation than inbred C57Bl/6 mice[J].Neuroscience,2011,190(190):67-74.
[5]Guo X,Geng M,Du G.Glucose transporter 1,distribution in the brain and in neural disorders:its relationship with transport of neuroactive drugs through the blood-brain barrier[J].Biochem Genet,2005,43(3-4):175.
[6]Nagamatsu S,Sawa H,Inoue N,et al.Gene expression of GLUT3glucose transporter regulated by glucose in vivo in mouse brain and in vitro in neuronal cell cultures from rat embryos[J].Biochem J,1994,300(Pt 1):125-131.
[7]中华医学会儿科学会新生儿学组.新生儿缺氧缺血性脑病诊断依据和临床分度[J].中国实用儿科杂志,2000,15(6):99-100.
[8]Van LH,de Haan TR,Offringa M,et al.Prognostic tests in term neonates with hypoxic-ischemic encephalopathy:a systematic review[J].Pediatrics,2013,131(1):88-98.
[9]许津莉,贾黎英,郭俊红,等.缺血缺氧性脑病新生儿免疫功能变化[J].临床荟萃,2012,27(16):1459-1461.
[10]刘敬,秦振庭.新生儿缺氧缺血性脑病的免疫学机制[J].国际儿科学杂志,2001,28(4):175-178.
[11]陆勤,王健,王薇,等.新生儿缺氧缺血性脑病细胞因子与细胞免疫的变化[J].中国小儿急救医学,2005,12(4):269-271.
[12]Zhou LJ,Tedder TF.CD14+Blood Monocytes can Differentiate into Functionally Mature CD83+Dendritic Cells[J].Proc Natl Acad Sci USA,1996,93(6):2588.
[13]Bauer S,Hartmann G.Toll-Like Receptors(TLRs)and Innate Immunity[J].Handb Exp Pharmacol,2008,183(5):267-276.
[14]梁桂娟,王迎涛,唐成和,等.Toll样受体4在新生大鼠缺氧缺血性脑损伤中的表达变化[J].中国新生儿科杂志,2016,31(3):222-225.
[15]黄晓雷,崔桂云.新生小鼠缺血缺氧性脑损伤中TLR4-TRIF信号通路的表达[J].中国实用神经疾病杂志,2012,15(9):16-18.
[16]喻婷,李晓瑜,邹晓萍,等.米诺环素对缺氧缺血脑损伤未成熟新生大鼠TLR4、NF-κBp65和TNF-α表达的影响[J].解剖学研究,2013(6):401-408.