HDAC6 mRNA在食管鳞癌组织中的表达及其临床意义
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摘要
目的检测HDAC6 mRNA在食管鳞癌组织及癌旁组织的表达情况,探讨其与食管癌临床病理特征相关性。方法应用RT-PCR(逆转录-聚合酶链反应)检测食管鳞癌组织及癌旁组织中HDAC6 mRNA在的表达情况。结果食管鳞癌组织中HDAC6 mRNA的表达水平高于癌旁组织;其表达情况与TNM分期及淋巴结转移程度呈正相关,而与患者其它临床及病理特征无关。结论 HDAC6 mRNA参与食管鳞癌的发生发展,将来可能作为食管癌新的生物学指标及药物作用靶点应用到患者的诊断及治疗中。
Objective To detect the expression of HDAC6 mRNA in esophageal squamous cell carcinoma(ESCC) tissues and adjacent noncancerous tissues,as well as to investigate the relation between HDAC6 mRNA's expression and clinical pathologic characteristics of ESCC. Methods The Semi-quantitative RT-PCR method was chosen to investigate the relative expression levels of HDAC6 mRNA in ESCC tissues and adjacent noncancerous tissues. Results The positive ratio and relative levels of HDAC6 mRNA in ESCC tissues is significantly higher than that in the adjacent noncancerous tissues. The positive ratio and relative levels of HDAC6 m RNA in ESCC tissues has positive correlation with TNM staging and lymph node metastasis. Conclusion HDAC6 mRNA is related to ESCC's development and metastasis and it may be act as one new biological target and drug target in diagnosis and therapy of ESCC.
Objective To detect the expression of HDAC6 mRNA in esophageal squamous cell carcinoma(ESCC) tissues and adjacent noncancerous tissues,as well as to investigate the relation between HDAC6 mRNA's expression and clinical pathologic characteristics of ESCC. Methods The Semi-quantitative RT-PCR method was chosen to investigate the relative expression levels of HDAC6 mRNA in ESCC tissues and adjacent noncancerous tissues. Results The positive ratio and relative levels of HDAC6 mRNA in ESCC tissues is significantly higher than that in the adjacent noncancerous tissues. The positive ratio and relative levels of HDAC6 m RNA in ESCC tissues has positive correlation with TNM staging and lymph node metastasis. Conclusion HDAC6 mRNA is related to ESCC's development and metastasis and it may be act as one new biological target and drug target in diagnosis and therapy of ESCC.
引文
[1]赫捷,邵康.中国食管癌流行病学现状、诊疗现状及未来对策[J].中国癌症杂志,2011(07):501-504.
[2]周脉耕,王晓风,胡建平.2004-2005年中国主要恶性肿瘤死亡的地理分布特点[J].中华预防医学杂志,2010,44(4):303-308.
[3]王涛,郭晓雷,张吉玉,等.山东大汶河流域居民恶性肿瘤死因调查[J].公共卫生,2007(4).
[4]钟钏,谭家驹,徐致祥.食管癌流行病学病因学研究进展[J].河南预防医学杂志,2011,22(1):1-10.
[5]周素丽,毛伟敏,凌志强,等.食管癌中hMSH2启动子区CpG岛过甲基化研究[J].肿瘤学杂志,2012(02):81-85.
[6]Fiskus W,Rao R,Fernandez P,et al.Molecular and biologic characterization and drug sensitivity of pan-histone deacetylase inhibitor-resistant acute myeloid leukemia cells[J]Blood,2008,112(7):2896-2905.
[7]Parmigiani R B,Xu W S,Venta-Perez G,et al.HDAC6 is a specific deacetylase of peroxiredoxins and is involved in redox regulation[J].Proc Natl Acad Sci U S A,2008,105(28):9633-9638.
[8]Verdel A,Khochbin S.Identification of a new family of higher eukaryotic histone deacetylases.Coordinate expression of differentiation-dependent chromatin modifiers[J].J Biol Chem,1999,274(4):2440-2445.
[9]Grozinger C M,Hassig C A,Schreiber S L.Three proteins define a class of human histone deacetylases related to yeast Hda1p[J].Proc Natl Acad Sci U S A,1999,96(9):4868-4873.
[10]Zhang Y,Gilquin B,Khochbin S,et al.Two catalytic domains are required for protein deacetylation[J].J Biol Chem,2006,281(5):2401-2404.
[11]Aoyagi S,Archer T K.Modulating molecular chaperone Hsp90 functions through reversible acetylation[J].Trends Cell Biol,2005,15(11):565-567.
[12]杨晋,赵绍林,杨新玲,等.HDAC6在食道癌中表达的初步研究[J].中国实验诊断学,2009,13(11):1577-1579.
[13]任月霞.组蛋白去乙酰化酶6(HDAC6)在食管鳞癌组织中的表达及其意义[D].2011.
[14]Lee Y S,Lim K H,Guo X,et al.The cytoplasmic deacetylase HDAC6 is required for efficient oncogenic tumorigenesis[J].Cancer Res,2008,68(18):7561-7569.
[15]曹金林,HDAC6在食管癌发生发展中的作用及其机制研究[D].2018.浙江大学.
[2]周脉耕,王晓风,胡建平.2004-2005年中国主要恶性肿瘤死亡的地理分布特点[J].中华预防医学杂志,2010,44(4):303-308.
[3]王涛,郭晓雷,张吉玉,等.山东大汶河流域居民恶性肿瘤死因调查[J].公共卫生,2007(4).
[4]钟钏,谭家驹,徐致祥.食管癌流行病学病因学研究进展[J].河南预防医学杂志,2011,22(1):1-10.
[5]周素丽,毛伟敏,凌志强,等.食管癌中hMSH2启动子区CpG岛过甲基化研究[J].肿瘤学杂志,2012(02):81-85.
[6]Fiskus W,Rao R,Fernandez P,et al.Molecular and biologic characterization and drug sensitivity of pan-histone deacetylase inhibitor-resistant acute myeloid leukemia cells[J]Blood,2008,112(7):2896-2905.
[7]Parmigiani R B,Xu W S,Venta-Perez G,et al.HDAC6 is a specific deacetylase of peroxiredoxins and is involved in redox regulation[J].Proc Natl Acad Sci U S A,2008,105(28):9633-9638.
[8]Verdel A,Khochbin S.Identification of a new family of higher eukaryotic histone deacetylases.Coordinate expression of differentiation-dependent chromatin modifiers[J].J Biol Chem,1999,274(4):2440-2445.
[9]Grozinger C M,Hassig C A,Schreiber S L.Three proteins define a class of human histone deacetylases related to yeast Hda1p[J].Proc Natl Acad Sci U S A,1999,96(9):4868-4873.
[10]Zhang Y,Gilquin B,Khochbin S,et al.Two catalytic domains are required for protein deacetylation[J].J Biol Chem,2006,281(5):2401-2404.
[11]Aoyagi S,Archer T K.Modulating molecular chaperone Hsp90 functions through reversible acetylation[J].Trends Cell Biol,2005,15(11):565-567.
[12]杨晋,赵绍林,杨新玲,等.HDAC6在食道癌中表达的初步研究[J].中国实验诊断学,2009,13(11):1577-1579.
[13]任月霞.组蛋白去乙酰化酶6(HDAC6)在食管鳞癌组织中的表达及其意义[D].2011.
[14]Lee Y S,Lim K H,Guo X,et al.The cytoplasmic deacetylase HDAC6 is required for efficient oncogenic tumorigenesis[J].Cancer Res,2008,68(18):7561-7569.
[15]曹金林,HDAC6在食管癌发生发展中的作用及其机制研究[D].2018.浙江大学.