均匀设计法优选淫羊藿多糖提取工艺
|
摘要
目的:优选淫羊藿多糖的提取工艺。方法:采用均匀设计法,以液料比、提取时间、提取温度、提取次数为考察因素,以多糖收率为评价指标,对淫羊藿多糖热水浸提工艺进行优化。结果:淫羊藿多糖最佳提取工艺为液料比25 m L·g-1、提取温度90℃、提取时间140 min、提取次数3次,多糖收率为1. 09%。结论:优选的淫羊藿多糖提取工艺稳定、可行,为淫羊藿多糖进一步研究开发提供实验依据。
Objective: To optimize the extraction process for Epimedium polysaccharide( EPS). Methods: The effects of liquid-material ratio,extraction time,extraction temperature and extraction times on polysaccharides extraction yields were investigated by uniform design method. The result was analyzed and the extracting process was optimized. Results: The optimized conditions were as follows: extracted 3 times with 25 times the amount of water at 90 ℃ and each for 140 min. The yield was 1. 09%. Conclusion: The optimum extraction process of EPS is stable and feasible,which could provide experimental basis for further research and development.
Objective: To optimize the extraction process for Epimedium polysaccharide( EPS). Methods: The effects of liquid-material ratio,extraction time,extraction temperature and extraction times on polysaccharides extraction yields were investigated by uniform design method. The result was analyzed and the extracting process was optimized. Results: The optimized conditions were as follows: extracted 3 times with 25 times the amount of water at 90 ℃ and each for 140 min. The yield was 1. 09%. Conclusion: The optimum extraction process of EPS is stable and feasible,which could provide experimental basis for further research and development.
引文
[1]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:327-328.
[2]王程成,苏嘉炎,蔡继炎,等.响应面优化朝鲜淫羊藿多糖提取条件及其肿瘤免疫活性研究[J].药学学报,2016,51(9):1464-1471.
[3]孟宪丽,李建亚,张艺,等.淫羊藿多糖对老年大鼠神经内分泌免疫调节作用的研究[J].中药药理与临床,1998,4(3):19-20.
[4]李秋莹,陈守哲,李波,等.淫羊藿多糖抗氧化性研究[J].亚太传统医药,2016,12(18):24-26.
[5]董娜,李倩,张贵强,等.淫羊藿多糖对甲型H1N1流感病毒裂解疫苗的免疫佐剂作用[J].国际药学研究杂志,2013,40(1):63-68.
[6]吕岫华,刘伟,郝冬梅,等.中药多糖对EB病毒早期抗原激活的抑制作用[J].北京工业大学学报,2010,36(1):92-97.
[7]陈建,郭辉,马瑞彬.淫羊藿多糖的精制实验研究[J].时珍国医国药,2006,17(7):1241-1242.
[8]于红梅.淫羊藿多糖提取工艺的优化及其对肉鸡免疫功能的调节作用[D].聊城:聊城大学,2015.
[9]姜虹,李新龙,李海平,等.响应面法优化淫羊藿多糖超声提取工艺的研究[J].长春师范学院学报,2013,32(2):59-64.
[10]刘晓鹏,姜宁,刘志彪,等.微波辅助提取淫羊藿多糖研究[J].亚太传统医药,2014,10(16):16-18.
[11]张曜武,王薇薇,王超,等.淫羊藿多糖的酶法除蛋白工艺研究[J].青岛科技大学学报(自然科学版),2014,35(2):167-169,173.
[12]侯丽丽,陈洪海,张守勤.朝鲜淫羊藿多糖的提取工艺研究[J].湖北农业科学,2015,54(1):145-147,151.
[13]王小梅,孙润广,张静,等.超声提取功率对麦冬多糖结构及抗氧化活性的影响[J].陕西师范大学学报(自然科学版),2013,41(3):68-75.
[14]张贵明,王继文,王文泽,等.朝鲜产东北红豆杉树皮中多糖提取工艺的比较研究[J].中国现代中药,2008,10(6):32-34.
[15]张庆红,马梅芳.硫酸-蒽酮法测定天冬中多糖含量[J].中国现代中药,2008,10(8):18-19,22.
[2]王程成,苏嘉炎,蔡继炎,等.响应面优化朝鲜淫羊藿多糖提取条件及其肿瘤免疫活性研究[J].药学学报,2016,51(9):1464-1471.
[3]孟宪丽,李建亚,张艺,等.淫羊藿多糖对老年大鼠神经内分泌免疫调节作用的研究[J].中药药理与临床,1998,4(3):19-20.
[4]李秋莹,陈守哲,李波,等.淫羊藿多糖抗氧化性研究[J].亚太传统医药,2016,12(18):24-26.
[5]董娜,李倩,张贵强,等.淫羊藿多糖对甲型H1N1流感病毒裂解疫苗的免疫佐剂作用[J].国际药学研究杂志,2013,40(1):63-68.
[6]吕岫华,刘伟,郝冬梅,等.中药多糖对EB病毒早期抗原激活的抑制作用[J].北京工业大学学报,2010,36(1):92-97.
[7]陈建,郭辉,马瑞彬.淫羊藿多糖的精制实验研究[J].时珍国医国药,2006,17(7):1241-1242.
[8]于红梅.淫羊藿多糖提取工艺的优化及其对肉鸡免疫功能的调节作用[D].聊城:聊城大学,2015.
[9]姜虹,李新龙,李海平,等.响应面法优化淫羊藿多糖超声提取工艺的研究[J].长春师范学院学报,2013,32(2):59-64.
[10]刘晓鹏,姜宁,刘志彪,等.微波辅助提取淫羊藿多糖研究[J].亚太传统医药,2014,10(16):16-18.
[11]张曜武,王薇薇,王超,等.淫羊藿多糖的酶法除蛋白工艺研究[J].青岛科技大学学报(自然科学版),2014,35(2):167-169,173.
[12]侯丽丽,陈洪海,张守勤.朝鲜淫羊藿多糖的提取工艺研究[J].湖北农业科学,2015,54(1):145-147,151.
[13]王小梅,孙润广,张静,等.超声提取功率对麦冬多糖结构及抗氧化活性的影响[J].陕西师范大学学报(自然科学版),2013,41(3):68-75.
[14]张贵明,王继文,王文泽,等.朝鲜产东北红豆杉树皮中多糖提取工艺的比较研究[J].中国现代中药,2008,10(6):32-34.
[15]张庆红,马梅芳.硫酸-蒽酮法测定天冬中多糖含量[J].中国现代中药,2008,10(8):18-19,22.