乌头属中药多糖研究进展
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摘要
乌头属植物是重要的药用植物资源,已经开发出草乌甲素等二萜生物碱类无成瘾性镇痛药。除二萜生物碱外,多糖亦是乌头属植物的主要活性成分。乌头属植物多糖具有增强免疫、降血糖、抗炎、抗肿瘤、抗氧化、降胆固醇等生物活性。该文对1986年以来文献报道的乌头属植物多糖的提取分离、含量测定及生物活性研究进行综述,为乌头属植物的研究开发提供参考。
Aconitum plants are important medicinal plant resources. Diterpenoid alkaloids such as bulleyaconitine A have been developed as non-addictive analgesics. In addition to diterpenoid alkaloids, polysaccharides are also the main active ingredients of Aconitum plants. The polysaccharides of Aconitum plants have the bioactivity of enhancing immunity, lowering blood sugar, anti-inflammation,anti-tumor, anti-oxidation and lowering cholesterol. In this paper, the extraction, separation, content determination and bioactivity of Aconitum polysaccharides reported in the literature since 1986 were reviewed, which could provide a reference for the research and development of Aconitum plants.
Aconitum plants are important medicinal plant resources. Diterpenoid alkaloids such as bulleyaconitine A have been developed as non-addictive analgesics. In addition to diterpenoid alkaloids, polysaccharides are also the main active ingredients of Aconitum plants. The polysaccharides of Aconitum plants have the bioactivity of enhancing immunity, lowering blood sugar, anti-inflammation,anti-tumor, anti-oxidation and lowering cholesterol. In this paper, the extraction, separation, content determination and bioactivity of Aconitum polysaccharides reported in the literature since 1986 were reviewed, which could provide a reference for the research and development of Aconitum plants.
引文
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[3]乌力吉特古斯,白学良,阿拉坦松布尔,等.蒙药草乌叶化学成分及临床研究进展[J].中草药,2006,37(3):472-474.
[4] NYIRIMIGABO E,XU Y,LI Y,et al. A review on phytochemistry,pharmacology and toxicology studies of Aconitum[J]. J Pharm Pharmacol,2015,67(1):1-19.
[5] GONZALEZ-COLOMA A,REINA M,MEDINAVEITIA A,et al.Structural diversity and defensive properties of norditerpenoid alkaloids[J]. J Chem Ecol,2004,30(7):1393-1408.
[6] KONNO C,SUZUKI Y,OISHI K,et al. Isolation and hypoglycemic activity of atractans A,B and C,glycans of Atractylodes japonica rhizomes[J]. Planta Med,1985(2):102-103.
[7]赵驰.附子多糖提取、分离、纯化、结构鉴定与免疫调节作用的研究[D].广州:中山大学,2007.
[8] ZHAO C,LI M,LUO Y,et al. Isolation and structural characterization of an immunostimulating polysaccharide from fuzi,Aconitum carmichaeli[J]. Carbohydrate Research,2006,341(4):485-491.
[9]施松善,王顺春.多糖生物活性研究进展[J].生命科学,2011,23(7):662-670.
[10] GAO TT,MA S,SONG JY,et al. Antioxidant and immunological activities of water-soluble polysaccharides from Aconitum kusnezoffii Reichb.[J]. Int J Biol Macromol,2011,49(4):580-586.
[11] ATAL C,SHARMA M,KAUL A,et al. Immunomodulating agents of plant origin. I:Preliminary screening[J]. J Ethnopharmacol,1986,18(2):133-141.
[12] LIANG DY,LI XQ,SHI XY,et al. The complement component C5a receptor mediates pain and inflammation in a postsurgical pain model[J]. Pain,2012,153(2):366-372.
[13]李晓军,蒋嘉烨,施松善,等.关白附中1,6-葡聚糖及其硫酸酯的抗补体活性[J].高等学校化学学报,2014,35(7):1423-1426.
[14]阮期平,赵莉,陈吉森.附子多糖FI的分离纯化及生物学活性研究[J].四川生理科学杂志,2000,22(2):40-41.
[15] KONNO C,MURAYAMA M,SUGIYAMA K,et al. Isolation and hypoglycemic activity of aconitans A,B,C and D,glycans of Aconitum carmichaeli roots[J]. Planta Med,1985(2):160-161.
[16] WANG BB,WANG JL,YUAN J,et al. Sugar composition analysis of Fuzi polysaccharides by HPLC-MS(n)and their protective effects on Schwann cells exposed to high glucose[J]. Molecules,2016,21(11):1496-1510.
[17] LI H,SUN M,XU J,et al. Immunological response in H22 transplanted mice undergoing Aconitum coreanum polysaccharide treatment[J]. Int J Biol Macromol,2013,55:295-300.
[18] LIANG M,LI S,SHEN B,et al. Anti-hepatocarcinoma effects of Aconitum coreanum polysaccharides[J]. Carbohydrate Polymers,2012,88(3):973-976.
[19] LIANG M,LIU J,JI H,et al. A Aconitum coreanum polysaccharide fraction induces apoptosis of hepatocellular carcinoma(HCC)cells via pituitary tumor transforming gene 1(PTTG1)-mediated suppression of the P13K/Akt and activation of p38 MAPK signaling pathway and displays antitumor activity in vivo[J]. Tumour Biol,2015,36(9):7085-7091.
[20] ROBINSON DR,WU YM,LONIGRO RJ,et al. Integrative clinical genomics of metastatic cancer[J]. Nature,2017,548(7667):297-303.
[21] ZHANG Y,WU W,KANG L,et al. Effect of Aconitum coreanum polysaccharide and its sulphated derivative on the migration of human breast cancer MDA-MB-435s cell[J]. Int J Biol Macromol,2017,103:477-483.
[22] SUN L,WANG Z,WANG H,et al. Polysaccharide sulphated derivative from Aconitum coreanum induces cell apoptosis in the human brain glioblastoma U87MG cell line via the NF-kappaB/Bcl-2 cell apoptotic signaling pathway[J]. Oncol Rep,2018,39(3):1469-1474.
[23] LI X,JIANG J,SHI SS,et al. A RG-Ⅱtype polysaccharide purified from Aconitum coreanum and their anti-inflammatory activity[J]. Plos One,2014,9(6):e99697.
[24] WADE CR,JACKSON PG,HIGHTON J,et al. Lipid peroxidation and malondialdehyde in the synovial fluid and plasma of patients with rheumatoid arthritis[J]. Clinica Chimica Acta,1987,164(3):245-250.
[25]郭憬憬,职润,张娜,等.关白附多糖体外抗氧化性研究[J].食品与生物技术学报,2007,26(1):43-45.
[26] LIU CH,WANG CH,XU ZL,et al. Isolation,chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water[J]. Process Biochem,2007,42(6):961-970.
[27]温爱平,杨来秀,王玉华,等.紫花高乌头多糖提取工艺及抗氧化活性的研究[J].中药材,2016,39(11):2579-2581.
[28] TSIAPALI E,WHALEY S,KALBFLEISCH J,et al. Glucans exhibit weak antioxidant activity,but stimulate macrophage free radical activity[J]. Free Radic Biol Med,2001,30(4):393-402.
[29] PRASAD KN,XIE H,HAO J,et al. Antioxidant and anticancer activities of 8-hydroxypsoralen isolated from wampee[Clausena lansium(Lour.)Skeels] peel[J]. Food Chem,2010,118(1):62-66.
[30]黄雄庆.附子多糖降血胆固醇作用及机制的研究[D].广州:中山大学,2008.
[31] LIAO L,ZHUANG X,LI W,et al. Polysaccharide from Fuzi protects against Ox-LDL-induced calcification of human vascular smooth muscle cells by increasing autophagic activity[J]. Mol Med Rep,2018,17(4):5109-5115.
[32]袁晶,徐晓婷,徐凌川.圆锥乌头多糖脱蛋白及抗HIV-1 RT活性研究[J].齐鲁药事,2010,29(5):303-306.
[33]哈文秀,利毛才让,热增才旦.藏药唐古特乌头中多糖的提取实验研究[J].安徽农业科学,2010,38(5):2577-2578.
[34]哈文秀,利毛才让.超声法提取藏药唐古特乌头中多糖的研究[J].化学与生物工程,2010,27(7):63-65.
[35]马鸿雁,刘小彬,李楠,等.附子粗多糖提取工艺的优化[J].时珍国医国药,2005,16(1):22-23.
[36]叶思勇,邵孟霞,陈智.乌头多糖的提取分离与含量测定[J].中国医药导报,2011,8(33):70-71.
[37]于永交,梁冬雪,张婷岚,等.响应面法优化关白附中多糖的提取工艺[J].食品与药品,2011,13(7):260-264.
[38]叶强,张丹,郭力.附子多糖提取纯化工艺研究[J].中药与临床,2013,4(2):29-31.
[39] TONG H,FENG K,ZHANG X,et al. Purification and chemical compositions of a proteoglycan isolated from Aconitum coreanum[J].Chem Nat Compd,2010,46(2):329-330.
[40]祝晓涛.关白附多糖提取纯化工艺及理化性质研究[D].延吉:延边大学,2011.
[41] ZHANG YL,GAO J,MIAO XZ,et al. Monosaccharide composition analysis of polysaccharides from Aconitum Kusnezoffii based on HPLC-MS[J]. Transylvanian Review,2017,1(14):1-5.
[42]孙玉军,陈彦,吴佳静,等.草乌多糖的分离纯化和组成性质研究[J].中国药学杂志,2000,35(11):11-13.
[43]阮期平,周立,赵莉.黄附子中性多糖和酸性蛋白多糖的分离、纯化与鉴定[J].中国生化药物杂志,2000,21(1):20-22.
[44]朱晓霞,罗学刚.天麻多糖水提及脱蛋白工艺优化[J].中药材,2007,30(6):724-726.
[45]钟方晓,任海华,李岩.多糖含量测定方法比较[J].时珍国医国药,2007,18(8):1916-1917.
[46]杨帆,郝爱国,徐恒瑰,等.附子多糖的含量测定[J].大连医科大学学报,2007,29(5):453-454.
[47]赵祥升,王惠,许源,等.江油附子多糖含量的测定[J].安徽农业科学,2009,37(2):650-651.
[48]冯凯,佟海滨,田丹,等.关白附中多糖含量的测定[J].北华大学学报(自然科学版),2009,10(5):436-438.
[49]舒晓燕,侯大斌,李凤.不同品种附子生物碱和多糖含量的比较[J].中国药房,2010,21(31):2916-2918.
[50]欧水平,王森,郑琴,等.乌头全植株各组织部位粗多糖含量的动态差异性考察[J].中国实验方剂学杂志,2013,19(5):7-9.
[51]吕永磊,王丹,李向日,等.附子多糖的含量测定[J].药物分析杂志,2011,31(5):835-838.
[52]吕永磊,卜海博,杨蕾,等.乌头母根、子根、须根多糖的比较[J].中国中药杂志,2011,31(9):1154-1157.
[53]赵莉,高长健,王刚,等.不同气候环境对乌头粗多糖含量的影响[J].绵阳师范高等专科学校学报,2000,19(5):49-51.
[54]赵祥升,侯大斌,舒晓燕.植物激素对附子多糖含量的影响[J].时珍国医国药,2009,20(9):2195-2196.
[55] WU Y,ANTONY S,MEITZLER JL,et al. Molecular mechanisms underlying chronic inflammation-associated cancers[J]. Cancer Lett,2014,345(2):164-173.
[2]李谦,过立农,郑健,等.乌头属药用植物的研究进展[J].药物分析杂志,2016,36(7):1129-1149.
[3]乌力吉特古斯,白学良,阿拉坦松布尔,等.蒙药草乌叶化学成分及临床研究进展[J].中草药,2006,37(3):472-474.
[4] NYIRIMIGABO E,XU Y,LI Y,et al. A review on phytochemistry,pharmacology and toxicology studies of Aconitum[J]. J Pharm Pharmacol,2015,67(1):1-19.
[5] GONZALEZ-COLOMA A,REINA M,MEDINAVEITIA A,et al.Structural diversity and defensive properties of norditerpenoid alkaloids[J]. J Chem Ecol,2004,30(7):1393-1408.
[6] KONNO C,SUZUKI Y,OISHI K,et al. Isolation and hypoglycemic activity of atractans A,B and C,glycans of Atractylodes japonica rhizomes[J]. Planta Med,1985(2):102-103.
[7]赵驰.附子多糖提取、分离、纯化、结构鉴定与免疫调节作用的研究[D].广州:中山大学,2007.
[8] ZHAO C,LI M,LUO Y,et al. Isolation and structural characterization of an immunostimulating polysaccharide from fuzi,Aconitum carmichaeli[J]. Carbohydrate Research,2006,341(4):485-491.
[9]施松善,王顺春.多糖生物活性研究进展[J].生命科学,2011,23(7):662-670.
[10] GAO TT,MA S,SONG JY,et al. Antioxidant and immunological activities of water-soluble polysaccharides from Aconitum kusnezoffii Reichb.[J]. Int J Biol Macromol,2011,49(4):580-586.
[11] ATAL C,SHARMA M,KAUL A,et al. Immunomodulating agents of plant origin. I:Preliminary screening[J]. J Ethnopharmacol,1986,18(2):133-141.
[12] LIANG DY,LI XQ,SHI XY,et al. The complement component C5a receptor mediates pain and inflammation in a postsurgical pain model[J]. Pain,2012,153(2):366-372.
[13]李晓军,蒋嘉烨,施松善,等.关白附中1,6-葡聚糖及其硫酸酯的抗补体活性[J].高等学校化学学报,2014,35(7):1423-1426.
[14]阮期平,赵莉,陈吉森.附子多糖FI的分离纯化及生物学活性研究[J].四川生理科学杂志,2000,22(2):40-41.
[15] KONNO C,MURAYAMA M,SUGIYAMA K,et al. Isolation and hypoglycemic activity of aconitans A,B,C and D,glycans of Aconitum carmichaeli roots[J]. Planta Med,1985(2):160-161.
[16] WANG BB,WANG JL,YUAN J,et al. Sugar composition analysis of Fuzi polysaccharides by HPLC-MS(n)and their protective effects on Schwann cells exposed to high glucose[J]. Molecules,2016,21(11):1496-1510.
[17] LI H,SUN M,XU J,et al. Immunological response in H22 transplanted mice undergoing Aconitum coreanum polysaccharide treatment[J]. Int J Biol Macromol,2013,55:295-300.
[18] LIANG M,LI S,SHEN B,et al. Anti-hepatocarcinoma effects of Aconitum coreanum polysaccharides[J]. Carbohydrate Polymers,2012,88(3):973-976.
[19] LIANG M,LIU J,JI H,et al. A Aconitum coreanum polysaccharide fraction induces apoptosis of hepatocellular carcinoma(HCC)cells via pituitary tumor transforming gene 1(PTTG1)-mediated suppression of the P13K/Akt and activation of p38 MAPK signaling pathway and displays antitumor activity in vivo[J]. Tumour Biol,2015,36(9):7085-7091.
[20] ROBINSON DR,WU YM,LONIGRO RJ,et al. Integrative clinical genomics of metastatic cancer[J]. Nature,2017,548(7667):297-303.
[21] ZHANG Y,WU W,KANG L,et al. Effect of Aconitum coreanum polysaccharide and its sulphated derivative on the migration of human breast cancer MDA-MB-435s cell[J]. Int J Biol Macromol,2017,103:477-483.
[22] SUN L,WANG Z,WANG H,et al. Polysaccharide sulphated derivative from Aconitum coreanum induces cell apoptosis in the human brain glioblastoma U87MG cell line via the NF-kappaB/Bcl-2 cell apoptotic signaling pathway[J]. Oncol Rep,2018,39(3):1469-1474.
[23] LI X,JIANG J,SHI SS,et al. A RG-Ⅱtype polysaccharide purified from Aconitum coreanum and their anti-inflammatory activity[J]. Plos One,2014,9(6):e99697.
[24] WADE CR,JACKSON PG,HIGHTON J,et al. Lipid peroxidation and malondialdehyde in the synovial fluid and plasma of patients with rheumatoid arthritis[J]. Clinica Chimica Acta,1987,164(3):245-250.
[25]郭憬憬,职润,张娜,等.关白附多糖体外抗氧化性研究[J].食品与生物技术学报,2007,26(1):43-45.
[26] LIU CH,WANG CH,XU ZL,et al. Isolation,chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water[J]. Process Biochem,2007,42(6):961-970.
[27]温爱平,杨来秀,王玉华,等.紫花高乌头多糖提取工艺及抗氧化活性的研究[J].中药材,2016,39(11):2579-2581.
[28] TSIAPALI E,WHALEY S,KALBFLEISCH J,et al. Glucans exhibit weak antioxidant activity,but stimulate macrophage free radical activity[J]. Free Radic Biol Med,2001,30(4):393-402.
[29] PRASAD KN,XIE H,HAO J,et al. Antioxidant and anticancer activities of 8-hydroxypsoralen isolated from wampee[Clausena lansium(Lour.)Skeels] peel[J]. Food Chem,2010,118(1):62-66.
[30]黄雄庆.附子多糖降血胆固醇作用及机制的研究[D].广州:中山大学,2008.
[31] LIAO L,ZHUANG X,LI W,et al. Polysaccharide from Fuzi protects against Ox-LDL-induced calcification of human vascular smooth muscle cells by increasing autophagic activity[J]. Mol Med Rep,2018,17(4):5109-5115.
[32]袁晶,徐晓婷,徐凌川.圆锥乌头多糖脱蛋白及抗HIV-1 RT活性研究[J].齐鲁药事,2010,29(5):303-306.
[33]哈文秀,利毛才让,热增才旦.藏药唐古特乌头中多糖的提取实验研究[J].安徽农业科学,2010,38(5):2577-2578.
[34]哈文秀,利毛才让.超声法提取藏药唐古特乌头中多糖的研究[J].化学与生物工程,2010,27(7):63-65.
[35]马鸿雁,刘小彬,李楠,等.附子粗多糖提取工艺的优化[J].时珍国医国药,2005,16(1):22-23.
[36]叶思勇,邵孟霞,陈智.乌头多糖的提取分离与含量测定[J].中国医药导报,2011,8(33):70-71.
[37]于永交,梁冬雪,张婷岚,等.响应面法优化关白附中多糖的提取工艺[J].食品与药品,2011,13(7):260-264.
[38]叶强,张丹,郭力.附子多糖提取纯化工艺研究[J].中药与临床,2013,4(2):29-31.
[39] TONG H,FENG K,ZHANG X,et al. Purification and chemical compositions of a proteoglycan isolated from Aconitum coreanum[J].Chem Nat Compd,2010,46(2):329-330.
[40]祝晓涛.关白附多糖提取纯化工艺及理化性质研究[D].延吉:延边大学,2011.
[41] ZHANG YL,GAO J,MIAO XZ,et al. Monosaccharide composition analysis of polysaccharides from Aconitum Kusnezoffii based on HPLC-MS[J]. Transylvanian Review,2017,1(14):1-5.
[42]孙玉军,陈彦,吴佳静,等.草乌多糖的分离纯化和组成性质研究[J].中国药学杂志,2000,35(11):11-13.
[43]阮期平,周立,赵莉.黄附子中性多糖和酸性蛋白多糖的分离、纯化与鉴定[J].中国生化药物杂志,2000,21(1):20-22.
[44]朱晓霞,罗学刚.天麻多糖水提及脱蛋白工艺优化[J].中药材,2007,30(6):724-726.
[45]钟方晓,任海华,李岩.多糖含量测定方法比较[J].时珍国医国药,2007,18(8):1916-1917.
[46]杨帆,郝爱国,徐恒瑰,等.附子多糖的含量测定[J].大连医科大学学报,2007,29(5):453-454.
[47]赵祥升,王惠,许源,等.江油附子多糖含量的测定[J].安徽农业科学,2009,37(2):650-651.
[48]冯凯,佟海滨,田丹,等.关白附中多糖含量的测定[J].北华大学学报(自然科学版),2009,10(5):436-438.
[49]舒晓燕,侯大斌,李凤.不同品种附子生物碱和多糖含量的比较[J].中国药房,2010,21(31):2916-2918.
[50]欧水平,王森,郑琴,等.乌头全植株各组织部位粗多糖含量的动态差异性考察[J].中国实验方剂学杂志,2013,19(5):7-9.
[51]吕永磊,王丹,李向日,等.附子多糖的含量测定[J].药物分析杂志,2011,31(5):835-838.
[52]吕永磊,卜海博,杨蕾,等.乌头母根、子根、须根多糖的比较[J].中国中药杂志,2011,31(9):1154-1157.
[53]赵莉,高长健,王刚,等.不同气候环境对乌头粗多糖含量的影响[J].绵阳师范高等专科学校学报,2000,19(5):49-51.
[54]赵祥升,侯大斌,舒晓燕.植物激素对附子多糖含量的影响[J].时珍国医国药,2009,20(9):2195-2196.
[55] WU Y,ANTONY S,MEITZLER JL,et al. Molecular mechanisms underlying chronic inflammation-associated cancers[J]. Cancer Lett,2014,345(2):164-173.