戈宝红麻叶片水提物的抗氧化作用
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摘要
为戈宝红麻天然保健品的开发利用奠定理论依据,采用回流提取法得戈宝红麻叶片水提物,通过体外测定其清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基、超氧自由基(O_2~-·)能力和还原能力,评价水提物的抗氧化能力;并考察10μg/mL、100μg/mL、500μg/mL和1 000μg/mL水提物对秀丽隐杆线虫的寿命、头部摆动、排泄周期、吞咽频率及体内抗氧化酶活力的影响。结果表明:戈宝红麻叶片水提物对DPPH和O_2~-·均表现出良好的清除效果,清除率均与浓度呈正相关;也具有良好的还原力,与水提物浓度呈剂量效应;10μg/mL、100μg/mL和500μg/mL水提物均能显著延长秀丽隐杆线虫的寿命、提高体内抗氧化酶活力、提高运动能力、缩短排泄周期并提高吞咽频率。适宜浓度的戈宝红麻叶片水提物具有一定的抗氧化作用。
The antioxidant capacity of the aqueous extract fromApocynum venetumleaves by a reflux extraction method was evaluated by determining its DPPH and O_2~-· scavenging capacity and reducing power and the effects of the aqueous extracts with four different concentrations(10μg/mL,100μg/mL,500μg/mL and 1 000μg/mL)on life span,head swing,excretion cycle,deglutition frequency and antioxidant enzyme activity of Caenorhabditis elegans in vivo to lay a theoretical basis for utilization and exploitation of A.venetum natural health product.Result:The aqueous extract from A.venetumleaves has a good scavenging effect on DPPH and O_2~-· and the scavenging rate is related to its concentration positively.The aqueous extract fromA.venetumleaves has a good reducing power and its reducing power increases linearly with concentration.The aqueous extract from A.venetum leaves with 10μg/mL,100μg/mL and 500μg/mL concentration can prolong life span of C.elegans,increase the antioxidant enzyme activity in vivo,improve its athletic ability,shorten excretion cycle and raise its deglutition frequency significantly.In conclusion,the aqueous extract from A.venetum leaves with the suitable concentration is of a certain antioxidation.
The antioxidant capacity of the aqueous extract fromApocynum venetumleaves by a reflux extraction method was evaluated by determining its DPPH and O_2~-· scavenging capacity and reducing power and the effects of the aqueous extracts with four different concentrations(10μg/mL,100μg/mL,500μg/mL and 1 000μg/mL)on life span,head swing,excretion cycle,deglutition frequency and antioxidant enzyme activity of Caenorhabditis elegans in vivo to lay a theoretical basis for utilization and exploitation of A.venetum natural health product.Result:The aqueous extract from A.venetumleaves has a good scavenging effect on DPPH and O_2~-· and the scavenging rate is related to its concentration positively.The aqueous extract fromA.venetumleaves has a good reducing power and its reducing power increases linearly with concentration.The aqueous extract from A.venetum leaves with 10μg/mL,100μg/mL and 500μg/mL concentration can prolong life span of C.elegans,increase the antioxidant enzyme activity in vivo,improve its athletic ability,shorten excretion cycle and raise its deglutition frequency significantly.In conclusion,the aqueous extract from A.venetum leaves with the suitable concentration is of a certain antioxidation.
引文
[1]作者不详.戈宝红麻简介[J].中国野生植物资源,2009,28(1):72.
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[8]张强,孙玉军,储俊,等.洋葱中黄酮类化合物的提取及其体外抗氧化活性研究[J].中国食品添加剂,2009(3):74-79.
[9]帕尔哈提·柔孜,阿依姑丽·艾合麦提,朱昆,等.玫瑰花瓣总黄酮和总多糖的体外抗氧化活性[J].食品科学,2013,34(11):138-141.
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[15]SHU H J,YU X M,WU Q L,et al.Pretreatment with paeonol prevents the adverse effects and alters thetranslocation ofmulti-walled carbonnanotubes in nematode Caenorhabditis elegans[J].RSC Advances,2015,5:8942-8951.
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[17]张佳婵,王昌涛,刘瑶,等.沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制[J].食品科学,2017,38(23):141-148.
[18]程艳,陈璐,米艳华,等.水稻抗氧化酶活性测定方法的比较研究[J].江西农业学报,2018,30(2):108-111.
[19]HUANG R T,HUANG Q,WU G L,et al.Evaluation of the antioxidant property and effects in Caenorhabditis elegans of Xiangxi flavor vinegar,a Hunan local traditional vinegar[J].Biomed&Biotechnol,2017,18(4):324-333.
[20]熊双丽,卢飞,史敏娟,等.DPPH自由基清除活性评价方法在抗氧化剂筛选中的研究进展[J].食品工业科技,2012,33(8):380-383.
[21]王罗玲,沈雪林,李亚峰,等.香青菜不同溶剂提取物抗氧化活性研究[J].食品研究与开发,2017,38(9):28-33.
[22]柴明艳.红觅菜提取物抗氧化活性的研究[J].食品研究与开发,2016,37(12):19-23.
[23]杨燚超,邢晓娟,王大勇.重金属暴露秀丽线虫中存在咽泵运动与寿命之间的显著关联[J].东南大学学报(医学版),2010,29(1):10-16.
[24]张佳婵,王昌涛,刘瑶,等.沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制[J].食品科学,2017,38(23):141-148.
[25]田立,郭晓农,孟杨,等.罗布麻多糖成分体外抗氧化活性研究[J].中兽医医药杂志,2016,35(4):5-8.
[2]肖正春,张卫明,孟繁杰,等.戈宝麻保健饮品研究进展[J].中国野生植物资源,2012,31(1):4-8.
[3]TAN Z J,YI Y J,WANG H Y,et al.Extraction,Preconcentration and Isolation of Flavonoids from Apocynum venetum L.Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an A-queous Biphasic System[J].Molecules,2016,21:262.
[4]张文彬,龚海英,张丽,等.天然产物抗氧化活性体内外评价方法[J].武警后勤学院学报(医学版),2013,22(11):1035-1038.
[5]GREERE L,BLANCOM A,GU L,et al.DNA methylation on N6-Adenine in C.elegans[J].Cell,2015,161(4):868-878.
[6]KOOPMAN M,MICHELS H,DANCYB M,et al.Ascreening-based platform for the assessment of cellular respiration in Caenorhabditi selegans[J].Nature Protocols,2016,11(10):1798-1816.
[7]徐悦,张卫明,马世宏,等.芫荽水提物的体外抗氧化活性和抑制α-葡萄糖苷酶作用的研究[J].中国调味品,2018,43(3):46-50.
[8]张强,孙玉军,储俊,等.洋葱中黄酮类化合物的提取及其体外抗氧化活性研究[J].中国食品添加剂,2009(3):74-79.
[9]帕尔哈提·柔孜,阿依姑丽·艾合麦提,朱昆,等.玫瑰花瓣总黄酮和总多糖的体外抗氧化活性[J].食品科学,2013,34(11):138-141.
[10]田立,郭晓农,孟杨,等.罗布麻多糖成分体外抗氧化活性研究[J].中兽医医药杂志,2016,35(4):5-8.
[11]WANG D,CAO M,DIN H J,et al.Methods for creating mutations in C.elegans that extend lifespan[J].Methods in MelecularBology,2013,1048:65-75.
[12]WANG D,WANG Y,SHEN L,et al.Confirmation of combinational effects of calcium with other metals in apaper recycling mill effluent on nematode lifespan with toxicity identification evaluation method[J].Environmental Science&Technology,2010,22(5):731-737.
[13]ZHANG W,LV T,LI M,et al.Beneficial effects of wheat gluten hydrolysate to extend lifespan and induce stress resistance in nematode Caenorhabditis elegans[J].PLoS One,2013,8(9):e74553.
[14]王晗,米生权.壳寡糖对秀丽隐杆线虫寿命的影响[J].食品科学,2015,36(1):229-233.
[15]SHU H J,YU X M,WU Q L,et al.Pretreatment with paeonol prevents the adverse effects and alters thetranslocation ofmulti-walled carbonnanotubes in nematode Caenorhabditis elegans[J].RSC Advances,2015,5:8942-8951.
[16]王艳菊,马建伟,王曦茁,等.葡萄籽原花青素对秀丽隐杆线虫抗衰老的影响[J].食品工业科技,2014,35(20):369-373.
[17]张佳婵,王昌涛,刘瑶,等.沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制[J].食品科学,2017,38(23):141-148.
[18]程艳,陈璐,米艳华,等.水稻抗氧化酶活性测定方法的比较研究[J].江西农业学报,2018,30(2):108-111.
[19]HUANG R T,HUANG Q,WU G L,et al.Evaluation of the antioxidant property and effects in Caenorhabditis elegans of Xiangxi flavor vinegar,a Hunan local traditional vinegar[J].Biomed&Biotechnol,2017,18(4):324-333.
[20]熊双丽,卢飞,史敏娟,等.DPPH自由基清除活性评价方法在抗氧化剂筛选中的研究进展[J].食品工业科技,2012,33(8):380-383.
[21]王罗玲,沈雪林,李亚峰,等.香青菜不同溶剂提取物抗氧化活性研究[J].食品研究与开发,2017,38(9):28-33.
[22]柴明艳.红觅菜提取物抗氧化活性的研究[J].食品研究与开发,2016,37(12):19-23.
[23]杨燚超,邢晓娟,王大勇.重金属暴露秀丽线虫中存在咽泵运动与寿命之间的显著关联[J].东南大学学报(医学版),2010,29(1):10-16.
[24]张佳婵,王昌涛,刘瑶,等.沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制[J].食品科学,2017,38(23):141-148.
[25]田立,郭晓农,孟杨,等.罗布麻多糖成分体外抗氧化活性研究[J].中兽医医药杂志,2016,35(4):5-8.