淫羊藿苷对正畸牙齿移动及牙周组织改建的影响
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摘要
目的探讨淫羊藿苷对大鼠正畸牙齿移动速度及张力侧牙周组织改建的影响。方法 24只健康雄性SD大鼠,依据淫羊藿苷的浓度随机分为淫羊藿苷1、3、5 mg/kg组(实验组)和对照组,自加力装置放置第1天起用不同浓度的淫羊藿苷灌胃,对照组灌同等体积的生理盐水,加力14 d后处死实验动物。制取上颌第一磨牙及周围牙周组织标本块,进行免疫组化染色和HE染色分析,测量上颌第一恒磨牙向近中移动的距离。用SPSS 17.0软件进行统计分析。结果与对照组比较,实验组RUNX2在张力侧牙周组织中的平均光密度值高于对照组,随着药物浓度的增加,RUNX2的平均光密度值逐渐增大(P<0.05)。牙齿移动的距离随着药物浓度的增加逐渐减少(P<0.05),在药物浓度为5 mg/kg时,牙齿移动的量最少。结论淫羊藿苷可以抑制正畸牙齿移动,增加张力侧牙周组织中RUNX2的表达量。
Objective To explore the effect of icariin on orthodontic tooth movement and the expression of RUNX2 in periodontal tissue. Methods Twenty-four healthy male Sprague-Dawley(SD)rats were randomly divided into four groups: icariin 1, 3, 5 mg/kg group and control group. Icariin of different doses was ig given to these rats from the first day the torque device was placed. The same volume of physiological saline was given to the control group. All the rats were sacrificed after the torque device was loaded 14 days. Immunohistochemical staining was used to determine the expression of RUNX2. The range of tooth movement was measured. SPSS 17.0 software was used for statistical analysis.Results The expression of RUNX2 in the experimental group was higher than in the control group. With the increase of drug concentration, the expression of RUNX2 was increased gradually(P<0.05). The range of tooth movement was reduced gradually. When the drug concentration was 5 mg/kg, tooth movement was minimized. Conclusion Icariin can restrain orthodontic tooth movement and increase the expression of RUNX2 on the tension side.
Objective To explore the effect of icariin on orthodontic tooth movement and the expression of RUNX2 in periodontal tissue. Methods Twenty-four healthy male Sprague-Dawley(SD)rats were randomly divided into four groups: icariin 1, 3, 5 mg/kg group and control group. Icariin of different doses was ig given to these rats from the first day the torque device was placed. The same volume of physiological saline was given to the control group. All the rats were sacrificed after the torque device was loaded 14 days. Immunohistochemical staining was used to determine the expression of RUNX2. The range of tooth movement was measured. SPSS 17.0 software was used for statistical analysis.Results The expression of RUNX2 in the experimental group was higher than in the control group. With the increase of drug concentration, the expression of RUNX2 was increased gradually(P<0.05). The range of tooth movement was reduced gradually. When the drug concentration was 5 mg/kg, tooth movement was minimized. Conclusion Icariin can restrain orthodontic tooth movement and increase the expression of RUNX2 on the tension side.
引文
[1]陈雅竹,管晓燕,肖茜文,等.中草药在正畸牙移动牙周组织改建中的作用[J].中国组织工程研究,2014,18(46):7 477-7 481.
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[4]Zhang J,Song J,Shao J.Icariin attenuates glucocorticoid induced bone deteriorations,hypocalcemia and hypercalciuria in mice[J].Int J Clin Exp Med,2015,8(5):7 306-7 314.
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[9]Yamaguchi M.RANK.RANKL.OPG during orthodontic tooth movement[J].Orthod Craniofac Res,2009,12(2):113-119.
[10]顾继航,王兰珠,张苗苗,等.在正畸力的作用下大鼠牙周膜张力侧Runx2与β-catenin的表达[J].空军医学杂志,2016,32(2):127-132.
[11]Komori T.Regulation of bone development and extracellular matrix protein genes by RUNX2[J].Cell Tissue Res,2010,339(1):189-195.
[12]Zhang MM,Feng DF,Shao P.et al.Nifedipine regulated periodontal ligament remodeling:An experimental study in rats[J].Acta Odontol Scand,2008,66(6):327-333.
[13]任旭升,徐杰,王旭霞,等.二膦酸盐局部注射对去势正畸牙大鼠牙周组织改建的影响[J].山东医药,2013,53(3):28-30.
[14]杨柳,尹宏,钱卫庆.淫羊藿对成骨细胞及破骨细胞影响的研究进展[J].上海中医药杂志,2015,49(2):94-96.
[15]Hsieh TP,Sheu SY,Sun JS,et al.Icariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs/NF-κB regulated HIF-1αand PGE2 synthesis[J].Phytomedicine,2011,18(2):176-185.
[16]Li GW,Xu Z,Chang SX,et al.Icariin prevents ovariectomyinduced bone loss and lowers marrow adipogenesis[J].Menopause,2014,21(9):1 007-1 016.
[17]Liu M,Zhang C,He RX,et al.Icariin associated with exercise therapy is an effective treatment for postmenopausal osteoporosis[J].Chin Med J,2012,125(10):1 784-1 789.
[18]周艳妮,曹宝成,蒋小龙,等.淫羊藿苷可抑制正畸致牙根的吸收[J].中国组织工程研究,2013,17(28):5 171-5 176.
[2]刘波,张睿,徐彭,等.淫羊藿对去卵巢大鼠骨质疏松的影响[J].中国实验方剂学杂志,2013,19(7):178-181.
[3]Zhang DC,Liu JL,Ding YB,et al.Icariin potentiates the antitumor activity of gemcitabine in gallbladder cancer by suppressing NF-κB[J].Acta Pharmacol Sin,2013,34(2):301-308.
[4]Zhang J,Song J,Shao J.Icariin attenuates glucocorticoid induced bone deteriorations,hypocalcemia and hypercalciuria in mice[J].Int J Clin Exp Med,2015,8(5):7 306-7 314.
[5]Wang F,Liu Z,Lin S,et al.Icariin enhances the healing of rapid palatal expansion induced root resorption in rats[J].Phytomedicine,2012,19(11):1 035-1 041.
[6]Li XF,Xu H,Zhao YJ,et al.Icariin Augments Bone Formation and Reverses the Phenotypes of Osteoprotegerin-Defi cient Mice through the Activation of Wnt/beta-Catenin-BMP Signaling[J].Evid Based Complement Alternat Med,2013:652 317.
[7]顾新丰,蒋垚.RUNX2的研究进展[J].医学分子生物学杂志,2006,3(1):52-54.
[8]Han JY,He H.Expression and function of osteogenic genes runt-related transcription factor 2 and osterix in orthodontic tooth movement in rats[J].Clin Exp Patho,2015,8(9):11 895-11 900.
[9]Yamaguchi M.RANK.RANKL.OPG during orthodontic tooth movement[J].Orthod Craniofac Res,2009,12(2):113-119.
[10]顾继航,王兰珠,张苗苗,等.在正畸力的作用下大鼠牙周膜张力侧Runx2与β-catenin的表达[J].空军医学杂志,2016,32(2):127-132.
[11]Komori T.Regulation of bone development and extracellular matrix protein genes by RUNX2[J].Cell Tissue Res,2010,339(1):189-195.
[12]Zhang MM,Feng DF,Shao P.et al.Nifedipine regulated periodontal ligament remodeling:An experimental study in rats[J].Acta Odontol Scand,2008,66(6):327-333.
[13]任旭升,徐杰,王旭霞,等.二膦酸盐局部注射对去势正畸牙大鼠牙周组织改建的影响[J].山东医药,2013,53(3):28-30.
[14]杨柳,尹宏,钱卫庆.淫羊藿对成骨细胞及破骨细胞影响的研究进展[J].上海中医药杂志,2015,49(2):94-96.
[15]Hsieh TP,Sheu SY,Sun JS,et al.Icariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs/NF-κB regulated HIF-1αand PGE2 synthesis[J].Phytomedicine,2011,18(2):176-185.
[16]Li GW,Xu Z,Chang SX,et al.Icariin prevents ovariectomyinduced bone loss and lowers marrow adipogenesis[J].Menopause,2014,21(9):1 007-1 016.
[17]Liu M,Zhang C,He RX,et al.Icariin associated with exercise therapy is an effective treatment for postmenopausal osteoporosis[J].Chin Med J,2012,125(10):1 784-1 789.
[18]周艳妮,曹宝成,蒋小龙,等.淫羊藿苷可抑制正畸致牙根的吸收[J].中国组织工程研究,2013,17(28):5 171-5 176.