亚硒酸钠对小鼠脾脏淋巴细胞体外增殖及细胞因子分泌的影响
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摘要
[目的]研究亚硒酸钠对小鼠脾脏淋巴细胞体外增殖及脾脏淋巴细胞中细胞因子分泌的影响,进而探讨亚硒酸钠调节机体免疫可能的作用途径。[方法]从BALB/c小鼠脾脏中分离淋巴细胞,采用MTS法检测不同浓度(0、10~(-8)、10~(-7)、10~(-6)和10~(-5) mol/L)的亚硒酸钠培养基培养48 h后的淋巴细胞增殖率并筛选出最适浓度的亚硒酸钠进行脾脏淋巴细胞培养,最后采用ELISA法检测培养48 h后培养液中IFN-γ、IL-1β、IL-4和IL-6的分泌量。[结果]与对照组相比,10~(-7) mol/L亚硒酸钠处理使淋巴细胞增殖率显著提高,且培养液中IFN-γ、IL-1β、IL-4和IL-6的分泌量分别为对照组的10.34、2.15、1.06和6.68倍(P<0.05)。[结论]亚硒酸钠可能通过调节脾脏淋巴细胞增殖和细胞因子的分泌来调节机体免疫功能。
[Objective]To study the effects of sodium selenite on the spleen lymphocyte's proliferation and the cytokines secretion of mice in vitro,and explore the possible immune regulation ways of sodium selenite.[Method]The lymphocyte was isolated from the spleen of BALB/c mice.MTS method was used to detect the proliferation rate of lymphocytes cultured in sodium selenite medium with different concentrations(0,10~(-8),10~(-7),10~(-6) and 10~(-5) mol/L) after 48 h and screen out the optimum concentration of sodium selenite for spleen lymphocyte culture.The secretion quantity of IFN-γ,IL-1β,IL-4 and IL-6 in culture supernatant after culturing 48 h were measured by ELISA.[Result]The proliferation rate of lymphocytes treated by 10~(-7) mol/mL sodium selenite was significantly increased compared with that in control group,and the secretion quantity of IFN-γ,IL-1β,IL-4 and IL-6 in culture supernatant increased by 10.34,2.15,1.06,and 6.68 times,respectively.[Conclusion]Sodium selenite may regulate the body immunity by regulating spleen lymphocyte's proliferation and secretion of cytokines.
[Objective]To study the effects of sodium selenite on the spleen lymphocyte's proliferation and the cytokines secretion of mice in vitro,and explore the possible immune regulation ways of sodium selenite.[Method]The lymphocyte was isolated from the spleen of BALB/c mice.MTS method was used to detect the proliferation rate of lymphocytes cultured in sodium selenite medium with different concentrations(0,10~(-8),10~(-7),10~(-6) and 10~(-5) mol/L) after 48 h and screen out the optimum concentration of sodium selenite for spleen lymphocyte culture.The secretion quantity of IFN-γ,IL-1β,IL-4 and IL-6 in culture supernatant after culturing 48 h were measured by ELISA.[Result]The proliferation rate of lymphocytes treated by 10~(-7) mol/mL sodium selenite was significantly increased compared with that in control group,and the secretion quantity of IFN-γ,IL-1β,IL-4 and IL-6 in culture supernatant increased by 10.34,2.15,1.06,and 6.68 times,respectively.[Conclusion]Sodium selenite may regulate the body immunity by regulating spleen lymphocyte's proliferation and secretion of cytokines.
引文
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[15] MISRA S,NIYOGI S.Selenite causes cytotoxicity in rainbow trout (Oncorhynchus mykiss) hepatocytes by inducing oxidative stress[J].Toxicology in vitro,2009,23(7):1249-1258.
[16] WANG W S,XU L,BRANDSMA J H,et al.Convergent transcription of interferon-stimulated genes by TNF-α and IFN-α augments antiviral activity against HCV and HEV[J].Scientific reports,2016,6(1):1-14.
[17] KUTTY R K,SAMUEL W,BOYCE K,et al.Proinflammatory cytokines decrease the expression of genes critical for RPE function[J].Mol Vis,2016,22:1156-1168.
[18] APTE R N,DOTAN S,ELKABETS M,et al.The involvement of IL-1 in tumorigenesis,tumor invasiveness,metastasis and tumor-host interactions[J].Cancer and metastasis reviews,2006,25(3):387-408.
[19] TANAKA T,NARAZAKI M,KISHIMOYO T.IL-6 in inflammation,immunity,and disease[J].Cold spring harbor perspectives in biology,2014,6(10):1-16.
[20] CARAMORI G,ADCOCK I M,DI STEFANO A,et al.Cytokine inhibition in the treatment of COPD[J].Int J Chron Obstruct Pulmon Dis,2014,9:397-412.
[2] BINTE HOSSAIN K F,RAHMAN M M,SIKDER M T,et al.Inhibitory effects of selenium on cadmium-induced cytotoxicity in PC12 cells via regulating oxidative stress and apoptosis[J].Food Chem Toxicol,2018,114:180-189.
[3] XU D N,TIAN Y B.Selenium and polysaccharides of Atractylodes macrocephala Koidz play different roles in improving the immune response induced by heat stress in chickens[J].Biological trace element research,2015,168(1):235-241.
[4] JELINEK P D,ELLIS T,WROTH R H,et al.The effect of selenium supplementation on immunity,and the establishment of an experimental Haemonchus contortus infection,in weaner Merino sheep fed a low selenium diet[J].Aust Vet J,1988,65(7):214-217.
[5] DELEZIE E,ROVERS M,VAN DER AA A,et al.Comparing responses to different selenium sources and dosages in laying hens[J].Poult Sci,2014,93(12):3083-3090.
[6] LESSON S,NAMKUNG H,CASTON L,et al.Comparison of selenium levels and sources and dietary fat quality in diets for broiler breeders and layer hens[J].Poult Sci,2008,87(12):2605-2612.
[7] LI B X,LIU Y,LI W Y,et al.Effect of selenium on ion profiles and antioxidant defense in mice livers[J].Biological terace element research,2018,184(1):127-135.
[8] RAO S V R,PRAKASH B,RAJU M V L N,et al.Effect of supplementing organic selenium on performance,carcass traits,oxidative parameters and immune responses in commercial broiler chickens[J].Asian-Australasian journal of animal sciences,2013,26(2):247-252.
[9] YANG Z J,LIU C,LIU C P,et al.Selenium deficiency mainly influences antioxidant selenoproteins expression in broiler immune organs[J].Biological trace element research,2016,172(1):209-221.
[10] HUANG Z,ROSE A H,HOFFMANN P R.The role of selenium in inflammation and immunity:From molecular mechanisms to therapeutic opportunities[J].Antioxidants & redox signaling,2012,16(7):705-743.
[11] REN F,CHEN X X,HESKETH J,et al.Selenium promotes T-cell response to TCR-stimulation and ConA,but not PHA in primary porcine splenocytes[J].PLoS One,2012,7(4):1-10.
[12] HPFFMANN P R,BERRY M J.The influence of selenium on immune responses[J].Molecular nutrition & food research,2008,52(11):1273-1280.
[13] HAWKES W C,KELLEY D S,TAYLOR P C.The effects of dietary selenium on the immune system in healthy men[J].Biol Trace Elem Res,2001,81(3):189-213.
[14] WEILLER M,LATTA M,KRESSE M,et al.Toxicity of nutritionally available selenium compounds in primary and transformed hepatocytes[J].Toxicology,2004,201(1/2/3):21-30.
[15] MISRA S,NIYOGI S.Selenite causes cytotoxicity in rainbow trout (Oncorhynchus mykiss) hepatocytes by inducing oxidative stress[J].Toxicology in vitro,2009,23(7):1249-1258.
[16] WANG W S,XU L,BRANDSMA J H,et al.Convergent transcription of interferon-stimulated genes by TNF-α and IFN-α augments antiviral activity against HCV and HEV[J].Scientific reports,2016,6(1):1-14.
[17] KUTTY R K,SAMUEL W,BOYCE K,et al.Proinflammatory cytokines decrease the expression of genes critical for RPE function[J].Mol Vis,2016,22:1156-1168.
[18] APTE R N,DOTAN S,ELKABETS M,et al.The involvement of IL-1 in tumorigenesis,tumor invasiveness,metastasis and tumor-host interactions[J].Cancer and metastasis reviews,2006,25(3):387-408.
[19] TANAKA T,NARAZAKI M,KISHIMOYO T.IL-6 in inflammation,immunity,and disease[J].Cold spring harbor perspectives in biology,2014,6(10):1-16.
[20] CARAMORI G,ADCOCK I M,DI STEFANO A,et al.Cytokine inhibition in the treatment of COPD[J].Int J Chron Obstruct Pulmon Dis,2014,9:397-412.