毛蕊花苷对大鼠递增大强度运动下骨骼肌损伤的保护作用研究
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摘要
目的:研究毛蕊花苷对递增大强度运动大鼠骨骼肌损伤的保护作用。方法:SD大鼠随机分为正常对照组(NC)、单纯运动组(SE)、运动+毛蕊花苷低剂量组(EVL)、运动+毛蕊花苷中剂量组(EVM)、运动+毛蕊花苷高剂量组(EVH)。采用递增大强度跑台运动疲劳模型。实验末,取血和骨骼肌检测各组大鼠血清CK酶活性和骨骼肌氧化应激指标及HSP70蛋白表达量,并用光学显微镜观察骨骼肌微细结构改变。结果:1)与SE组相比,毛蕊花苷各剂量组明显减少了运动引起的血清CK活性的上升(P<0.05或P<0.01),减轻了运动所致的骨骼肌微细结构的异常,且EVH组的效果要好于EVL组和EVM组。2)与SE组相比,毛蕊花苷各剂量组显著抑制了运动导致的大鼠骨骼肌的氧化应激水平(P<0.05或P<0.01),其中EVH组抗氧化损伤的效果要好于EVL组和EVM组。3)与SE组相比,毛蕊花苷剂量组进一步诱导了运动引起的HSP70的高表达(P<0.05或P<0.01),其中以EVH组的蛋白表达增加更为显著(P<0.05)。结论:毛蕊花苷对运动大鼠骨骼肌损伤有良好的改善作用,显示出毛蕊花苷有明显抗骨骼肌氧化应激和减轻运动性骨骼肌微细结构损伤的保护作用,其中高剂量补充组的改善效果要优于其他剂量组,这可能与其能更好地诱导运动大鼠骨骼肌内源性保护蛋白(HSP70)表达以发挥HSP70保护效应有关。
Objective: To investigate protective effect of verbascoside on skeletal muscle injury induced exercise in trained rats. Methods: Sprague-Dawley(SD) rats were randomly divided into 5 groups: normal group(NC), simple exercise group(SE), exercise+verbascoside low dose group(EVL), exercise+verbascoside middle dose group(EVM) andexercise+verbascoside high dose group(EVH) with the mode of high-intensity increasing treadmill exercise fatigue. The serum CK was measured from the blood samples,and oxidative stress indexs and HSP70 protein expression were measured from skeletal muscle. And light microscope was employed to observe morphology changes of skeletal muscle in the experiment end. Results: 1) Compared to SE group,verbascoside treatment declined the leakage of creatine kinase induced exercise( P<0.05 or P<0.01) and skeletal muscle ultrastructural malformation. Among this,the improvement effect of EVH group was better than the two groups. 2) Compared to SE group,verbascoside treatment evidently alleviated oxidative stress by inhibiting lipiding effect of peroxidation( P<0.05 or P<0.01),and the improvement effect of EVH group was better than the two groups. 3) Compared to SE group,verbascoside treatment induced significantly the high HSP70 expression in the exercise rats( P<0.05 or P<0.01). Conclusion: Verbascoside exhibited protective role in exercise-induced injuries of skeletal muscle,and the improvement effect of high dose supplement was better than other supplement groups,which may be related to improving the endogenous protective protein( HSP70) expression in exercise rats.
Objective: To investigate protective effect of verbascoside on skeletal muscle injury induced exercise in trained rats. Methods: Sprague-Dawley(SD) rats were randomly divided into 5 groups: normal group(NC), simple exercise group(SE), exercise+verbascoside low dose group(EVL), exercise+verbascoside middle dose group(EVM) andexercise+verbascoside high dose group(EVH) with the mode of high-intensity increasing treadmill exercise fatigue. The serum CK was measured from the blood samples,and oxidative stress indexs and HSP70 protein expression were measured from skeletal muscle. And light microscope was employed to observe morphology changes of skeletal muscle in the experiment end. Results: 1) Compared to SE group,verbascoside treatment declined the leakage of creatine kinase induced exercise( P<0.05 or P<0.01) and skeletal muscle ultrastructural malformation. Among this,the improvement effect of EVH group was better than the two groups. 2) Compared to SE group,verbascoside treatment evidently alleviated oxidative stress by inhibiting lipiding effect of peroxidation( P<0.05 or P<0.01),and the improvement effect of EVH group was better than the two groups. 3) Compared to SE group,verbascoside treatment induced significantly the high HSP70 expression in the exercise rats( P<0.05 or P<0.01). Conclusion: Verbascoside exhibited protective role in exercise-induced injuries of skeletal muscle,and the improvement effect of high dose supplement was better than other supplement groups,which may be related to improving the endogenous protective protein( HSP70) expression in exercise rats.
引文
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[11]叶春,罗晶,许豪文,等.预热休克对大鼠骨骼肌运动性损伤的保护作用[J].体育科学,2005,2(9):53-55,82.
[12]朱洪竹,肖国强.预热适应对大鼠离心运动骨骼肌形态结构及HSP70表达的影响[J].体育科学,2011,31(12):73-78.
[13]朱洪竹,肖国强,朱梅菊,等.毛蕊花苷和马蒂苷对递增大强度运动大鼠骨骼肌细胞线粒体钙含量和肌浆网Ca2+-ATP酶活性的影响[J].广州体育学院学报,2012,32(4):109-113.
[14]张海平,高岩.离心运动致大鼠骨骼肌微损伤形态学及血清CK活性变化的研究[J].沈阳体育学院学报,2009,28(3):62-66.
[15]Staib J L,Tumer N,Powers S K. Increased temperature and protein oxidation lead to HSP72 mRNA and protein accumulation in the in vivo exercised rat heart[J]. Exp Physiol,2009,94(1):71-80.
[16]Hernando R,Manso R. Muscle fibre stress in response to exercise Synthesis,accumulation and isoform transitions of 70-kDa heat shock proteins[J].Eur J Biochem,1997,243(1-2):460-467.
[17]朱梅菊,高顺生,李红.螺旋藻复方对递增大负荷运动小鼠肝脏、心肌、骨骼肌的保护作用和HSP70表达的关系[J].天津体育学院学报,2005,20(3):35-37.
[18]Gonzales B,Manso R. Induction,modification and accumulation of HSP70s in the rat liver after acute exercise:early and late responses[J].J Physiol,2004,556(Pt2):369-385.
[19]LUC HEH SNOECKX,RICHARD N,et al.Heat shock proteins and cardiovascular Patho[J].Physiol Rev,2001,81:1461-1497.
[20]Murphy M E. The HSP70 family and cancer[J].Carcinogensis,2013,34(6):1181-1188.
[21]郑妩媚,王福文.热休克蛋白70及其在运动中作用研究进展[J],食品与药品,2015,17(3):223-227.
[22]关放,于兰,杨云.马先蒿属植物的研究进展[J].西北药学杂志,2006,21(3):142-144.
[23]Li J,Zheng R L,Liu Z M,et al. Scavenging effects of phenylpropanoid glycosides on superoxide and its antioxidative effects[J]. Acta Pharmacol Sin,1992,13:427-430.
[24]Bedirli A,Sakrak O,Muhtaroglu S,et al.Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70[J].J Surg Res,2004,122(1):96-102.
[2]朱洪竹,朱梅菊,等.毛蕊花苷和马蒂苷对递增大强度运动大鼠心肌和骨骼肌细胞线粒体过氧化损伤的保护作用[J].井冈山大学学报(自然科学版),2012,33(6):81-85.
[3]yzewski Z,Gregorczyk K P,Szulc-Dabrowska L,et al.Cooperation between heat shock proteins in organizing of proteins spatial structure[J].Postepy Hig Med Dosw(online),2014,68:793-807.
[4]Gehrmann M,Cervello M,Montalto G,et al. Heat shock protein 70 serum levels differ significantly in patients with chronic hepatitis,liver cirrhosis,and hepatocellular carcinoma[J]. Front Immunol,2014(5):307.
[5]Gou X,Yu TY,Zhou X,et al. Review of the mechanism of muscle fatigue and injury[J].CJTCMP,2016,31(7):2720-2724.
[6]汶希,潘华山等.大鼠运动性疲劳模型的建立[J].中国实验动物学报,2009,17,(5):368-372.
[7]Wang S Y,Camp M J,Ehlenfeldt M K. Antioxidant capacity and α-glucosidasa inhibitory activity in peel and flesh of blueberry (Vaccinium spp.) cultivars[J].Food Chemistry,2012,132(4):1759-1768.
[8]Fehrenbach E,Niess A M,Schlotz E,et al. Transcriptional and translational regulation of heat shock proteins in leukocytes of en durance runners[J]. J Appl Physiol (1985),2000,89(2):704-710.
[9]曹庆雷,何建伟,杨金龙.远红外陶瓷微珠对大鼠一次性力竭运动骨骼肌CK、CK-MM、LDH指标的影响[J].体育学刊,2013,20(3):125-129.
[10]袁青.血清肌酸激酶的运动训练负荷监控作用研究述评[J].体育学刊,2007,14 (6):40 -43.
[11]叶春,罗晶,许豪文,等.预热休克对大鼠骨骼肌运动性损伤的保护作用[J].体育科学,2005,2(9):53-55,82.
[12]朱洪竹,肖国强.预热适应对大鼠离心运动骨骼肌形态结构及HSP70表达的影响[J].体育科学,2011,31(12):73-78.
[13]朱洪竹,肖国强,朱梅菊,等.毛蕊花苷和马蒂苷对递增大强度运动大鼠骨骼肌细胞线粒体钙含量和肌浆网Ca2+-ATP酶活性的影响[J].广州体育学院学报,2012,32(4):109-113.
[14]张海平,高岩.离心运动致大鼠骨骼肌微损伤形态学及血清CK活性变化的研究[J].沈阳体育学院学报,2009,28(3):62-66.
[15]Staib J L,Tumer N,Powers S K. Increased temperature and protein oxidation lead to HSP72 mRNA and protein accumulation in the in vivo exercised rat heart[J]. Exp Physiol,2009,94(1):71-80.
[16]Hernando R,Manso R. Muscle fibre stress in response to exercise Synthesis,accumulation and isoform transitions of 70-kDa heat shock proteins[J].Eur J Biochem,1997,243(1-2):460-467.
[17]朱梅菊,高顺生,李红.螺旋藻复方对递增大负荷运动小鼠肝脏、心肌、骨骼肌的保护作用和HSP70表达的关系[J].天津体育学院学报,2005,20(3):35-37.
[18]Gonzales B,Manso R. Induction,modification and accumulation of HSP70s in the rat liver after acute exercise:early and late responses[J].J Physiol,2004,556(Pt2):369-385.
[19]LUC HEH SNOECKX,RICHARD N,et al.Heat shock proteins and cardiovascular Patho[J].Physiol Rev,2001,81:1461-1497.
[20]Murphy M E. The HSP70 family and cancer[J].Carcinogensis,2013,34(6):1181-1188.
[21]郑妩媚,王福文.热休克蛋白70及其在运动中作用研究进展[J],食品与药品,2015,17(3):223-227.
[22]关放,于兰,杨云.马先蒿属植物的研究进展[J].西北药学杂志,2006,21(3):142-144.
[23]Li J,Zheng R L,Liu Z M,et al. Scavenging effects of phenylpropanoid glycosides on superoxide and its antioxidative effects[J]. Acta Pharmacol Sin,1992,13:427-430.
[24]Bedirli A,Sakrak O,Muhtaroglu S,et al.Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70[J].J Surg Res,2004,122(1):96-102.