Isolation and Identification of Canine Enterococcus faecalis
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摘要
With the development of the urban pet industry, suppurative diseases of companion animals are increasing day by day. To explore its associated pathogens, this study was conducted on etilolgical identification from a case of a canine uterus with pus. Bacteriological detection methods were used to isolate and purify the collected disease materials, such as smear staining, microscopic examination and biochemical identification. Then, 16S rRNA molecular sequence analysis was used to identify the isolate. It was found that the morphological features of the isolated strain and the biochemical results were consistent with Enterococcus faecalis. The 16S rRNA of the isolate was also as high as 99% homologous with the reference stains. Drug susceptibility test showed that the isolate was resistant to tetracycline, penicillin G, minocycline, erythromycin, ciprofloxacin, norfloxacin, vancomycin, levofloxacin, clindamycin, polymyxin B, chloramphenicol, compound sulfamethoxazole, and clarithromycin, and only susceptible to oxacillin and nitrofurantoin. These results provided reference for the treatment of this type of multidrug-resistant enterococcal infection.
With the development of the urban pet industry, suppurative diseases of companion animals are increasing day by day. To explore its associated pathogens, this study was conducted on etilolgical identification from a case of a canine uterus with pus. Bacteriological detection methods were used to isolate and purify the collected disease materials, such as smear staining, microscopic examination and biochemical identification. Then, 16S rRNA molecular sequence analysis was used to identify the isolate. It was found that the morphological features of the isolated strain and the biochemical results were consistent with Enterococcus faecalis. The 16S rRNA of the isolate was also as high as 99% homologous with the reference stains. Drug susceptibility test showed that the isolate was resistant to tetracycline, penicillin G, minocycline, erythromycin, ciprofloxacin, norfloxacin, vancomycin, levofloxacin, clindamycin, polymyxin B, chloramphenicol, compound sulfamethoxazole, and clarithromycin, and only susceptible to oxacillin and nitrofurantoin. These results provided reference for the treatment of this type of multidrug-resistant enterococcal infection.
With the development of the urban pet industry, suppurative diseases of companion animals are increasing day by day. To explore its associated pathogens, this study was conducted on etilolgical identification from a case of a canine uterus with pus. Bacteriological detection methods were used to isolate and purify the collected disease materials, such as smear staining, microscopic examination and biochemical identification. Then, 16S rRNA molecular sequence analysis was used to identify the isolate. It was found that the morphological features of the isolated strain and the biochemical results were consistent with Enterococcus faecalis. The 16S rRNA of the isolate was also as high as 99% homologous with the reference stains. Drug susceptibility test showed that the isolate was resistant to tetracycline, penicillin G, minocycline, erythromycin, ciprofloxacin, norfloxacin, vancomycin, levofloxacin, clindamycin, polymyxin B, chloramphenicol, compound sulfamethoxazole, and clarithromycin, and only susceptible to oxacillin and nitrofurantoin. These results provided reference for the treatment of this type of multidrug-resistant enterococcal infection.
引文
[1]WANG YB,CHEN LY,HU H,et al.Development of a multiplex PCRassay for detection of swine-originated E.faecalis and E.faecium isolates[J].Chinese Journal of Veterinary Science,2011,31(8):1123-1127.(in Chinese)
[2]MALIK RK,MONTECALVO MA,REALE MR,et al.Epidemiology and control of vancomycin-resistant enterococci in a regional neonatal intensive care unit[J].Pediatr Infect Dis J,1999,18(4):352.
[3]TOLEDO-ARANA A,VALLE J,SOLANO C,et al.The enterococcal surface protein,Esp,is involved in Enterococcus faecalis biofilm formation[J].Appl Environ Microbiol,2001,67(10):4538-4545.
[4]CRETI R,IMPERI M,BERTUCCINI L,et al.Surver for virulence determinants among Enterococcus faecalis isolated from different sources[J].JMed Microbiol,2004,53:13-20.
[5]LIU YF,YANG JD,ZHAO RL.Research progress of animal diseases caused by Enterococcus faecalis[J].Journal of Domestic Animal Ecology,2013,34(11):75-77.(in Chinese)
[6]WANG YB,CHEN LY,ZHANG HY,et al.Identification and determination of virulence gene for different isolates of E.faecalis originated from infective piglet[J].Chinese Journal of Veterinary Science,2010,30(5):615-619.(in Chinese)()
[7]ANA RF,ANA PT,CARLA N,et al.Global spread of the hylemf colonization virulence gene plasmids of the Enterococcus farcium CC17 polyclonal sub cluster[J].Chemother,2010,54(6):2660-2665.
[8]WANG SL,LI YF,LIU QJ,et al.Drug resistance of swine Enterococcus faecalis in hunan province[J].Chinese Journal of Animal Infectious Diseases,2015,23(2):41-46.(in Chinese)
[9]HU GX,LIAO M,PENG YY,et al.Veterinary microbiology experiment course[M].Beijing:China Agriculture Press,2006.(in Chinese)
[10]CUI BA,NIU ZX,REN J,et al.Animal microbiology[M].Beijing:China Agriculture Press,2005.(in Chinese)
[11]JIANG DY,LIN XF,WANG BY,et al.Clinical distribution and drug resistance analysis of hospital infection en-terococci[J].China Modern Doctor,2015,53(35):99-102.(in Chinese)
[12]SHI CY.Isolation of bacteria from 43 cases of canine pyometra and the histopathology and immunohistochemical changes of uterine wall[D].Yangzhou:Yangzhou University,2016.(in Chinese)
[13]HU FP,ZHU DM,WANG X.2011 CHINET surveillance of bacterial resistance in China[J].Chinese Journal of Infection and Chemotherapy,2014,14(5):365-374.(in Chinese)
[14]LYU N,GAO Y.Advances in research on pathogenic factor of Enterococcus faecalis[J].Journal of Inner Mongolia University for Nationalities:Natural Sciences,2015,30(4):323-326.(in Chinese)
[15]WANG J,WANG XH,XU H.Antimicrobial resistance and dissemination of multidrug resistant organisms-A review[J].Acta microbiologica Sinica,2016,56(11):1671-1679.(in Chinese)
[2]MALIK RK,MONTECALVO MA,REALE MR,et al.Epidemiology and control of vancomycin-resistant enterococci in a regional neonatal intensive care unit[J].Pediatr Infect Dis J,1999,18(4):352.
[3]TOLEDO-ARANA A,VALLE J,SOLANO C,et al.The enterococcal surface protein,Esp,is involved in Enterococcus faecalis biofilm formation[J].Appl Environ Microbiol,2001,67(10):4538-4545.
[4]CRETI R,IMPERI M,BERTUCCINI L,et al.Surver for virulence determinants among Enterococcus faecalis isolated from different sources[J].JMed Microbiol,2004,53:13-20.
[5]LIU YF,YANG JD,ZHAO RL.Research progress of animal diseases caused by Enterococcus faecalis[J].Journal of Domestic Animal Ecology,2013,34(11):75-77.(in Chinese)
[6]WANG YB,CHEN LY,ZHANG HY,et al.Identification and determination of virulence gene for different isolates of E.faecalis originated from infective piglet[J].Chinese Journal of Veterinary Science,2010,30(5):615-619.(in Chinese)()
[7]ANA RF,ANA PT,CARLA N,et al.Global spread of the hylemf colonization virulence gene plasmids of the Enterococcus farcium CC17 polyclonal sub cluster[J].Chemother,2010,54(6):2660-2665.
[8]WANG SL,LI YF,LIU QJ,et al.Drug resistance of swine Enterococcus faecalis in hunan province[J].Chinese Journal of Animal Infectious Diseases,2015,23(2):41-46.(in Chinese)
[9]HU GX,LIAO M,PENG YY,et al.Veterinary microbiology experiment course[M].Beijing:China Agriculture Press,2006.(in Chinese)
[10]CUI BA,NIU ZX,REN J,et al.Animal microbiology[M].Beijing:China Agriculture Press,2005.(in Chinese)
[11]JIANG DY,LIN XF,WANG BY,et al.Clinical distribution and drug resistance analysis of hospital infection en-terococci[J].China Modern Doctor,2015,53(35):99-102.(in Chinese)
[12]SHI CY.Isolation of bacteria from 43 cases of canine pyometra and the histopathology and immunohistochemical changes of uterine wall[D].Yangzhou:Yangzhou University,2016.(in Chinese)
[13]HU FP,ZHU DM,WANG X.2011 CHINET surveillance of bacterial resistance in China[J].Chinese Journal of Infection and Chemotherapy,2014,14(5):365-374.(in Chinese)
[14]LYU N,GAO Y.Advances in research on pathogenic factor of Enterococcus faecalis[J].Journal of Inner Mongolia University for Nationalities:Natural Sciences,2015,30(4):323-326.(in Chinese)
[15]WANG J,WANG XH,XU H.Antimicrobial resistance and dissemination of multidrug resistant organisms-A review[J].Acta microbiologica Sinica,2016,56(11):1671-1679.(in Chinese)