C57BL/6小鼠上颌第一磨牙拔牙创愈合过程观察分析
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摘要
目的建立C57BL/6雌性小鼠拔牙创模型,观察第一磨牙拔除后的愈合过程。方法采用自制器械拔除小鼠上颌第一磨牙,应用体式显微镜、Micro-CT及HE染色等方法观察并记录拔牙后1、3、7、10、14、21、28、35 d共8个时间点拔牙创软组织及骨组织的愈合情况。结果小鼠拔牙后14 d拔牙窝表面软组织完全愈合;拔牙后7 d拔牙窝底部出现新生骨质,14 d时牙槽窝被新生骨质充填满,21～28 d时拔牙窝新生骨质继续改建,骨密度显著增加,35 d时新生骨质完成重塑改建。结论本研究详细阐明了正常小鼠上颌第一磨牙拔牙创愈合过程的阶段特性及时间,为利用小鼠拔牙创构建各种疾病模型的研究提供了详尽的参考。
Objective To establish a model of tooth extraction with C57 BL/6 mice,and observe the healing process after removal of the maxillary first molar. Methods The maxillary first molars were removed by surgical procedure. The changes of soft tissue and bone tissue at 8 different time points after tooth extraction were observed and recorded by stereomicroscope,Micro-CT and HE staining. Results The soft tissue on the surface of the extraction socket was completely healed 14 d after tooth extraction. New bone appeared at the bottom of the extraction socket 7 d after tooth extraction,and the extraction socket was fully filled at 14 d. After that,bone remodeling continued at 21 to 28 d,and the bone density increased significantly. At 35 d,the new bone had completed the remodeling. Conclusion This study states in detail the features at each time points in the healing process of the extraction of maxillary first molars in normal mice,and provides some basic data support for other studies based on the C57 BL/6 mouse extraction model.
Objective To establish a model of tooth extraction with C57 BL/6 mice,and observe the healing process after removal of the maxillary first molar. Methods The maxillary first molars were removed by surgical procedure. The changes of soft tissue and bone tissue at 8 different time points after tooth extraction were observed and recorded by stereomicroscope,Micro-CT and HE staining. Results The soft tissue on the surface of the extraction socket was completely healed 14 d after tooth extraction. New bone appeared at the bottom of the extraction socket 7 d after tooth extraction,and the extraction socket was fully filled at 14 d. After that,bone remodeling continued at 21 to 28 d,and the bone density increased significantly. At 35 d,the new bone had completed the remodeling. Conclusion This study states in detail the features at each time points in the healing process of the extraction of maxillary first molars in normal mice,and provides some basic data support for other studies based on the C57 BL/6 mouse extraction model.
引文
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[11]Pang P,Shimo T,Takada H,et al.Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction[J].Biochem Biophys Res Commun,2015,467(1):21-26.
[12]Cardaropoli G,Araujo M,Lindhe J.Dynamics of bone tissue formation in tooth extraction sites.An experimental study in dogs[J].J Clin Periodontol,2003,30(9):809-818.
[13]Bi Y,Gao Y,Ehirchiou D,et al.Bisphosphonates cause osteonecrosis of the jaw-like disease in mice[J].Am J Pathol,2010,177(1):280-290.
[2]Kim I,Ki H,Lee W,et al.The effect of systemically administered bisphosphonates on bony healing after tooth extraction and osseointegration of dental implants in the rabbit maxilla[J].Int J Oral Maxillofac Implants,2013,28(5):1194-1200.
[3]Allen MR,Chu TG,Ruggiero SL.Absence of exposed bone following dental extraction in beagle dogs treated with 9 months of high-dose zoledronic acid combined with dexamethasone[J].JOral Maxillofac Surg,2013,71(6):1017-1026.
[4]Kim S,Williams DW,Lee C,et al.IL-36 Induces bisphosphonate-related osteonecrosis of the jaw-like lesions in mice by inhibiting TGF-β-mediated collagen expression[J].J Bone Miner Res,2017,32(2):309-318.
[5]Soundia A,Hadaya D,Esfandi N,et al.Osteonecrosis of the jaws(ONJ)in mice after extraction of teeth with periradicular disease[J].Bone,2016,90:133-141.
[6]Matsuura Y,Atsuta I,Ayukawa Y,et al.Therapeutic interactions between mesenchymal stem cells for healing medication-related osteonecrosis of the jaw[J].Stem Cell Res Ther,2016,7(1):119.
[7]Yeh K,Popowics T,Rafferty K,et al.The effects of tooth extraction on alveolar bone biomechanics in the miniature pig,Sus scrofa[J].Arch Oral Biol,2010,55(9):663-669.
[8]Bodner L,Kaffe I,Littner MM,et al.Extraction site healing in rats.A radiologic densitometric study[J].Oral Surg Oral Med Oral Pathol Oral Radiol,1993,75(3):367-372.
[9]Vieira AE,Repeke CE,Ferreira Junior Sde B,et al.Intramembranous bone healing process subsequent to tooth extraction in mice:micro-computed tomography,histomorphometric and molecular characterization[J].PLoS One,2015,10(5):e0128021.
[10]Avivi-Arber L,Avivi D,Perez M,et al.Impaired bone healing at tooth extraction sites in CD24-deficient mice:A pilot study[J].PLoS One,2018,13(2):e0191665.
[11]Pang P,Shimo T,Takada H,et al.Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction[J].Biochem Biophys Res Commun,2015,467(1):21-26.
[12]Cardaropoli G,Araujo M,Lindhe J.Dynamics of bone tissue formation in tooth extraction sites.An experimental study in dogs[J].J Clin Periodontol,2003,30(9):809-818.
[13]Bi Y,Gao Y,Ehirchiou D,et al.Bisphosphonates cause osteonecrosis of the jaw-like disease in mice[J].Am J Pathol,2010,177(1):280-290.