棉花果枝节间长短性状内源激素的差异以及外施激素对其影响
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摘要
【目的】进行棉花果枝节间长度研究对于棉花株型的塑造具有重要意义。【方法】本研究通过对果枝节间长短不同的两个陆地棉中2549和中5081测定内源激素以及喷施不同的外源激素,探究棉花不同长度果枝节间的内源激素差异和喷施外源激素对棉花果枝节间长度的影响;同时对两个材料的果枝节间组织进行切片处理观察其细胞水平上的差异。【结果】果枝节间长的中2549内源激素IAA、GA_3和ABA的含量都明显高于果枝节间短的中5081;喷施外源激素IAA、GA_3和ABA都能使棉花果枝节间长度有一定程度的伸长;与中5081相比,中2549的果枝节间组织细胞明显较大,但细胞数量明显较少。【结论】本研究发现了不同棉花果枝节间长度材料在内源激素以及细胞水平上的差异,为棉花果枝节间长度分子机理探究奠定了基础。
[Objective] It is of great significance for shaping plant architecture, strengthening the research on cotton fruiting branch internode length. [Method] In this research, two upland cotton materials Zhong2549(long branch internode) and Zhong5081(short branch internode) were used for the study of the endogenous hormone differences by measuring endogenous hormone of the fruiting branch internode of Zhong2549 and Zhong5081; and for the study of the influence to the internode length by spraying exogenous hormone to Zhong2549 and Zhong5081. The paraffin section technique was used to detect the cell morphology of the fruiting branch internode of Zhong2549 and Zhong5081. [Result] that the content of endogenous hormone IAA,GA_3 and ABA in Zhong2549 were apparently higher than its in Zhong5081. Spraying exogenous hormone IAA, GA_3 and ABA promoted the fruiting branch internode elongation. The section result showed that the cell size of Zhong549 was bigger than Zhong5081, and the cell density of Zhong2548 was less than Zhong5081. These indicated that the endogenous hormones which affect the fruiting branch internode length and the differences of different length fruiting branch internode in cellular level. [Conclusion]This study can provide some foundation and reference for the later research.
[Objective] It is of great significance for shaping plant architecture, strengthening the research on cotton fruiting branch internode length. [Method] In this research, two upland cotton materials Zhong2549(long branch internode) and Zhong5081(short branch internode) were used for the study of the endogenous hormone differences by measuring endogenous hormone of the fruiting branch internode of Zhong2549 and Zhong5081; and for the study of the influence to the internode length by spraying exogenous hormone to Zhong2549 and Zhong5081. The paraffin section technique was used to detect the cell morphology of the fruiting branch internode of Zhong2549 and Zhong5081. [Result] that the content of endogenous hormone IAA,GA_3 and ABA in Zhong2549 were apparently higher than its in Zhong5081. Spraying exogenous hormone IAA, GA_3 and ABA promoted the fruiting branch internode elongation. The section result showed that the cell size of Zhong549 was bigger than Zhong5081, and the cell density of Zhong2548 was less than Zhong5081. These indicated that the endogenous hormones which affect the fruiting branch internode length and the differences of different length fruiting branch internode in cellular level. [Conclusion]This study can provide some foundation and reference for the later research.
引文
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[10]何业华,方少秋,胡中沂,等.菠萝体细胞胚发育过程的形态学和解剖学研究[J].园艺学报,2012,39(1):57-63.He Yehua,Fang Shaoqiu,Hu Zhongyi,et al.Morphological and anatomical analysis of pineapple somatic embryogenesis[J].Acta Horticulturae Sinica,2012,39(1):57-63.
[11]赵云,李涛,陈增洁.含羞草石蜡切片的制作[J].宁夏农林科技,2011,52(4):45-46.Zhao Yun,Li Tao,Chen Zengjie.Manufaction of paraffin section on Mimosa pudica[J].Ningxia Journal of Agriculture and Forestry Science and Technology,2011,52(4):45-46..
[12]朱倩,董美芳,袁王俊,等.‘天香台阁’桂花花芽分化及其台阁形成过程的观察[J].园艺学报,2012,39(2):315-322.Zhu Qian,Dong Meifang,Yuan Wangjun,et al.Studies on flower bud differentiation and leaflike proliferate-flower budof osmanthus fragrans‘Tianxiang Taige’[J].Acta Horticulturae Sinica,2012,39(2):315-322.
[13]Hamano M,Yamato Y,Yamazaki H,et al.Endogenous gibberellins and their effects on flowering and stem elongation in cabbage(Brassica oleracea var.capitata)[J].The Journal of Horticultural Science and Biotechnology,2002,77(2):220-225
[14]季兰,杨淑琴,马洪丽,等.长穗颈(eui)水稻上部节间伸长与植物激素的关系[J].应用与环境生物学报,2005,11(6):660-664.Ji Lan,Yang Shuqin,Ma Hongli,et al.Relation between upper internode elongation of rice with eui gene and plant hormones[J].Chinese Journal of Applied and Environmental Biology,2005,11(6):660-664.
[15]刘大同,荆彦平,陈晶晶,等.水稻的侧根发育及其影响因素[J].作物学报,2014,40(8):1403-1411.Liu Datong,Jing Yanping,Chen Jingjing,et al.Rice lateral root development and its impact factors[J].Acta Agronomica Sinica,2014,40(8):1403-1411.
[16]Phillips A L.Gibberellins in Arabidopsis[J].Plant Physiology and Biochemistry,1998,36(1):115-124.
[17]Zhang C,Bousquet A,Harris J M.Abscisic acid and LATD/NIP modulate root elongation via reactive oxygen species in Medicago truncatula[J].Plant Physiology,2014.DOI:10.1104/pp.114.248542.
[18]Mc Adam S A M,Brodribb T J,Ross J J.Shoot-derived abscisic acid promotes root growth[J].Plant Cell and Environment,2016,39(3):652-659.
[19]Antoni R,Gonzalez-Guzman M,Rodriguez L,et al.PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root[J].Plant Physiology,2013,161(2):931-941.
[20]Ondzighi-Assoume C A,Chakraborty S,Harris J M.Environmental nitrate stimulates abscisic acid accumulation in Arabidopsis root tips by releasing it from inactive stores[J].The Plant Cell,2016,28(3):729-745.
[21]刘剑锋,张春吉,程云清,等.ABA及其合成抑制剂钨酸钠处理对平榛胚珠发育的影响[J].园艺学报,2013,40(2):213-220.Liu Jianfeng,Zhang Chunji,Cheng Yunqing,et al.Effects of ABA and its synthesis inhibitor sodium tungstate treatments on ovule development of Corylus heterophylla[J].Acta Horticulturae Sinica,2013,40(2):213-220.
[22]肖丹曦.植物激素ABA和GA对紫球藻生长的影响[D].广州:暨南大学,2009.Xiao Danxi.Effects of abscisic acid and gibberellin on growth of Porhyridiu sp.[D].Guangzhou:Jinan University,2009.
[23]杨晓婉,郑国琦,杨涓,等.宁夏枸杞果实内源激素的变化及其与细胞壁成分和相关酶的关系[J].植物学报,2014,49(1):30-40.Yang Xiaowan,Zheng Guoqi,Yang Juan,et al.Changes in endogenous hormone contents and cell wall component,degrading enzyme activity and their relation in Lycium barbarum[J].Chinese Bulletin of Botany,2014,49(1):30-40.
[24]马霓,刘丹,张春雷,等.植物生长调节剂对油菜生长及冻害后光合作用和产量的调控效应[J].作物学报,2009,35(7):1336-1343.Ma Ni,Liu Dan,Zhang Chunlei,et al.Regulation effects of exogenous hormone s on growth and photosynthesis andyield of rapeseed(Brassica napus L.)after frozen[J].Acta Agronomica Sinica,2009,35(7):1336-1343.
[25]Yin C,Gan L,Ng D,et al.Decreased panicle-derived indole-3-acetic acid reduces gibberellin A1 level in the uppermost internode,causing panicle enclosure in male sterile rice Zhenshan 97A[J].Journal of Experimental Botany,2007,58(10):2441-2449.
[26]刘拥海,俞乐,丁君辉,等.植物激素对分枝发育的协同调控作用研究进展[J].植物生理学报,2012,48(10):941-948.Liu Yonghai,Yu Le,Ding Junhui,et al.Research progress in synergistic regulatory roles of phytohormones in shoot branching[J].Plant Physiology Journal,2012,48(10):941-948.
[2]马俊凯.全程机械化是发展棉花生产的根本出路[J].中国棉麻流通经济,2014(1):18-20.Ma Junkai.Fundamental way for cotton production is the mechanization[J].The Marketing of Cotton and Jute of China,2014(1):18-20.
[3]黄君成,周欣,熊宜勤.实用植物激素学[M].桂林:广西师范大学出版社,1998:5-15.Huang Diancheng,Zhou Xin,Xiong Yiqin.Practical plant hormones[M].Guilin:Guangxi Normal University Press,1998:5-15.
[4]岳川,曾建明,章志芳,等.茶树中植物激素研究进展[J].茶叶科学,2012,32(5):382-392.Yue Chuan,Zeng Jianming,Zhang Zhifang,et al.Research progress in the phytohormone of tea plant(Camellia sinensis)[J].Journal of Tea Science,2012,32(5):382-392.
[5]路文静.植物生理学[M].北京:中国林业出版社,2011:190.Lu Wenjing.Plant physiology[M].Beijing:China Forestry Publishing,2011:190.
[6]李鹏程,董合林,刘爱忠,等.外源激素对棉花前期生长发育的影响[J].棉花学报,2015,25(3):278-282.Li Pengcheng,Dong Helin,Liu Aizhong,et al.Effect of exogenous hormones on cotton(Gossypium hirsutum L.)growth and development during the early growth period[J].Cotton Science,2015,25(3):278-282.
[7]程建平,赵锋,游爱兵,等.赤霉素喷施量及时期对水稻穗层整齐度和产量的影响[J].华中农业大学学报,2011,3(6):657-662.Cheng Jianping,Zhao Feng,You Aibing,et al.Gibberellin spraying quantity and period of uniformity and yield of rice spike layer[J].Journal of Huazhong Agricultural University,2011,3(6):657-662.
[8]王丽芳,姚丽娟,杨燕萍,等.赤霉素和温度对彩色马蹄莲生长开花的影响[J].浙江农业科学,2014(5):699-700.Wang Lifang,Yao Lijuan,Yang Yanping,et al.Gibberellic acid and temperature on the influence of color calla lily grows[J].Journal of Zhejiang Agricultural Science,2014(5):699-700.
[9]Müller M,Munné-Bosch S.Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry[J/OL].Plant Methods,2011,7:37.http://www.plantmethods.com/content/7/1/37.DOI:10.1186/1746-4811-7-37.
[10]何业华,方少秋,胡中沂,等.菠萝体细胞胚发育过程的形态学和解剖学研究[J].园艺学报,2012,39(1):57-63.He Yehua,Fang Shaoqiu,Hu Zhongyi,et al.Morphological and anatomical analysis of pineapple somatic embryogenesis[J].Acta Horticulturae Sinica,2012,39(1):57-63.
[11]赵云,李涛,陈增洁.含羞草石蜡切片的制作[J].宁夏农林科技,2011,52(4):45-46.Zhao Yun,Li Tao,Chen Zengjie.Manufaction of paraffin section on Mimosa pudica[J].Ningxia Journal of Agriculture and Forestry Science and Technology,2011,52(4):45-46..
[12]朱倩,董美芳,袁王俊,等.‘天香台阁’桂花花芽分化及其台阁形成过程的观察[J].园艺学报,2012,39(2):315-322.Zhu Qian,Dong Meifang,Yuan Wangjun,et al.Studies on flower bud differentiation and leaflike proliferate-flower budof osmanthus fragrans‘Tianxiang Taige’[J].Acta Horticulturae Sinica,2012,39(2):315-322.
[13]Hamano M,Yamato Y,Yamazaki H,et al.Endogenous gibberellins and their effects on flowering and stem elongation in cabbage(Brassica oleracea var.capitata)[J].The Journal of Horticultural Science and Biotechnology,2002,77(2):220-225
[14]季兰,杨淑琴,马洪丽,等.长穗颈(eui)水稻上部节间伸长与植物激素的关系[J].应用与环境生物学报,2005,11(6):660-664.Ji Lan,Yang Shuqin,Ma Hongli,et al.Relation between upper internode elongation of rice with eui gene and plant hormones[J].Chinese Journal of Applied and Environmental Biology,2005,11(6):660-664.
[15]刘大同,荆彦平,陈晶晶,等.水稻的侧根发育及其影响因素[J].作物学报,2014,40(8):1403-1411.Liu Datong,Jing Yanping,Chen Jingjing,et al.Rice lateral root development and its impact factors[J].Acta Agronomica Sinica,2014,40(8):1403-1411.
[16]Phillips A L.Gibberellins in Arabidopsis[J].Plant Physiology and Biochemistry,1998,36(1):115-124.
[17]Zhang C,Bousquet A,Harris J M.Abscisic acid and LATD/NIP modulate root elongation via reactive oxygen species in Medicago truncatula[J].Plant Physiology,2014.DOI:10.1104/pp.114.248542.
[18]Mc Adam S A M,Brodribb T J,Ross J J.Shoot-derived abscisic acid promotes root growth[J].Plant Cell and Environment,2016,39(3):652-659.
[19]Antoni R,Gonzalez-Guzman M,Rodriguez L,et al.PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root[J].Plant Physiology,2013,161(2):931-941.
[20]Ondzighi-Assoume C A,Chakraborty S,Harris J M.Environmental nitrate stimulates abscisic acid accumulation in Arabidopsis root tips by releasing it from inactive stores[J].The Plant Cell,2016,28(3):729-745.
[21]刘剑锋,张春吉,程云清,等.ABA及其合成抑制剂钨酸钠处理对平榛胚珠发育的影响[J].园艺学报,2013,40(2):213-220.Liu Jianfeng,Zhang Chunji,Cheng Yunqing,et al.Effects of ABA and its synthesis inhibitor sodium tungstate treatments on ovule development of Corylus heterophylla[J].Acta Horticulturae Sinica,2013,40(2):213-220.
[22]肖丹曦.植物激素ABA和GA对紫球藻生长的影响[D].广州:暨南大学,2009.Xiao Danxi.Effects of abscisic acid and gibberellin on growth of Porhyridiu sp.[D].Guangzhou:Jinan University,2009.
[23]杨晓婉,郑国琦,杨涓,等.宁夏枸杞果实内源激素的变化及其与细胞壁成分和相关酶的关系[J].植物学报,2014,49(1):30-40.Yang Xiaowan,Zheng Guoqi,Yang Juan,et al.Changes in endogenous hormone contents and cell wall component,degrading enzyme activity and their relation in Lycium barbarum[J].Chinese Bulletin of Botany,2014,49(1):30-40.
[24]马霓,刘丹,张春雷,等.植物生长调节剂对油菜生长及冻害后光合作用和产量的调控效应[J].作物学报,2009,35(7):1336-1343.Ma Ni,Liu Dan,Zhang Chunlei,et al.Regulation effects of exogenous hormone s on growth and photosynthesis andyield of rapeseed(Brassica napus L.)after frozen[J].Acta Agronomica Sinica,2009,35(7):1336-1343.
[25]Yin C,Gan L,Ng D,et al.Decreased panicle-derived indole-3-acetic acid reduces gibberellin A1 level in the uppermost internode,causing panicle enclosure in male sterile rice Zhenshan 97A[J].Journal of Experimental Botany,2007,58(10):2441-2449.
[26]刘拥海,俞乐,丁君辉,等.植物激素对分枝发育的协同调控作用研究进展[J].植物生理学报,2012,48(10):941-948.Liu Yonghai,Yu Le,Ding Junhui,et al.Research progress in synergistic regulatory roles of phytohormones in shoot branching[J].Plant Physiology Journal,2012,48(10):941-948.