摘要
为探讨黄芩茎叶、藤茶及两者联合应用对金黄色葡萄球菌和痢疾杆菌的抑制作用,研究采用微量肉汤稀释法分别测定黄芩茎叶和藤茶对金黄色葡萄球菌和痢疾杆菌的最小抑菌浓度(Minimal inhibit concentration,MIC)及时间-杀菌曲线;采用棋盘式微量稀释法测定两者联合使用后的FIC指数。结果显示,黄芩茎叶和藤茶对金黄色葡萄球菌的MIC分别为1、0.25 mg/mL,对痢疾杆菌的MIC分别为4、0.50 mg/mL。两者联合使用,对金黄色葡萄球菌和痢疾杆菌的FIC指数分别为0.75、0.625,表现出相加的作用效果。研究提示基于联合使用,可进一步提高黄芩茎叶的抑菌效力,为实现黄芩茎叶的资源化利用提供科学依据。
To study the antibacterial effect of combination of the stem and leave of Scutellaria baicalensis(SLSB) and Ampelopsis grossedentata(AG) aginst Staphylococcus aureus and Shigella dysenteriae,the micro broth dilution method was used to detect their MIC values.The time-bactericidal curves of SLSB and AG against S.aureus and S.dysenteriae were established as well.A checkerboard method and the fractional inhibitory concentration index(FIC index) were used to evaluate the interaction between SLSB and AG.The results showed that the MIC values of SLSB and AG against S.aureus were 1 mg/mL and 0.25 mg/mL,while against S.dysenteriae were 4 mg/mL and 0.50 mg/mL,respectively.And the FIC indices of combined SLSB and AG,0.750 for S.aureus and 0.625 for S.dysenteriae,revealed their additive interaction against the tested bacterials.The present experimental results thus indicated the potential to increase the antibacterial efficacy of SLSB by rational combination strategy,providing a practical way for the rational utilization of SLSB resource.
引文
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