黄芩茎叶和藤茶抑菌作用及其联合应用研究
|
摘要
为探讨黄芩茎叶、藤茶及两者联合应用对金黄色葡萄球菌和痢疾杆菌的抑制作用,研究采用微量肉汤稀释法分别测定黄芩茎叶和藤茶对金黄色葡萄球菌和痢疾杆菌的最小抑菌浓度(Minimal inhibit concentration,MIC)及时间-杀菌曲线;采用棋盘式微量稀释法测定两者联合使用后的FIC指数。结果显示,黄芩茎叶和藤茶对金黄色葡萄球菌的MIC分别为1、0.25 mg/mL,对痢疾杆菌的MIC分别为4、0.50 mg/mL。两者联合使用,对金黄色葡萄球菌和痢疾杆菌的FIC指数分别为0.75、0.625,表现出相加的作用效果。研究提示基于联合使用,可进一步提高黄芩茎叶的抑菌效力,为实现黄芩茎叶的资源化利用提供科学依据。
To study the antibacterial effect of combination of the stem and leave of Scutellaria baicalensis(SLSB) and Ampelopsis grossedentata(AG) aginst Staphylococcus aureus and Shigella dysenteriae,the micro broth dilution method was used to detect their MIC values.The time-bactericidal curves of SLSB and AG against S.aureus and S.dysenteriae were established as well.A checkerboard method and the fractional inhibitory concentration index(FIC index) were used to evaluate the interaction between SLSB and AG.The results showed that the MIC values of SLSB and AG against S.aureus were 1 mg/mL and 0.25 mg/mL,while against S.dysenteriae were 4 mg/mL and 0.50 mg/mL,respectively.And the FIC indices of combined SLSB and AG,0.750 for S.aureus and 0.625 for S.dysenteriae,revealed their additive interaction against the tested bacterials.The present experimental results thus indicated the potential to increase the antibacterial efficacy of SLSB by rational combination strategy,providing a practical way for the rational utilization of SLSB resource.
To study the antibacterial effect of combination of the stem and leave of Scutellaria baicalensis(SLSB) and Ampelopsis grossedentata(AG) aginst Staphylococcus aureus and Shigella dysenteriae,the micro broth dilution method was used to detect their MIC values.The time-bactericidal curves of SLSB and AG against S.aureus and S.dysenteriae were established as well.A checkerboard method and the fractional inhibitory concentration index(FIC index) were used to evaluate the interaction between SLSB and AG.The results showed that the MIC values of SLSB and AG against S.aureus were 1 mg/mL and 0.25 mg/mL,while against S.dysenteriae were 4 mg/mL and 0.50 mg/mL,respectively.And the FIC indices of combined SLSB and AG,0.750 for S.aureus and 0.625 for S.dysenteriae,revealed their additive interaction against the tested bacterials.The present experimental results thus indicated the potential to increase the antibacterial efficacy of SLSB by rational combination strategy,providing a practical way for the rational utilization of SLSB resource.
引文
1 Chinese Pharmacopoeia Commission.Pharmacopoeia of the People’s Republic of China:Vol((中华人民共和国药典:第一部)[M].Beijing:China Medical Science Press,2015:301-302.
2 He CN,Peng Y,Xiao W,et al.Comparative study chemical composition in aerial parts and roots of Scutellaria baicalensis[J].Mod Chin Med(中国现代中药),2011,13:32-35.
3 Deng SH,Wang HM,Liu YHStudy on antibacterial activity of scutellaria baicalensis stem-leaf total flavonoid[J].J Chengde Med Coll(承德医学院学报),2008,25:324-325.
4 Zhao TH,Deng SH,Yang HS,et al.Study of antibacterial activity of active fraction from stems and leaves of Scutellaria baicalensis Georgi[J].Chin Pharmacol Bull(中国药理学通报),2007,23:882-886.
5 Shi GX,Shao J,Wang TM,et,al.New advance in studies on antimicrobal activity of Scutellaria baicalensis and its effective ingredients[J].Chin J Chin Mater Med(中国中药),2014,39:3713-3718.
6 Zhang YS,Yang WL,Xiong HP,et al Basic constituent of Ampelopsis grossedentata[J].Nat Prod Res Dev(天然产物研究与开发),2001,13:46-48.
7 Zhou QY,Chen J,Fang JG,et al.Research progress on antibacterial effects of Ampelopsis grossedentata[J].Chin Tradit Herb Drugs(中草药),2017,48:4819-4825.
8 Xu FX,Liu SY,Lan TF,et al.Pollution situation and coping strategies of foodborne pathogens.[J]Food Res Dev(食品研究与开发),2014,1:98-101.
9 Zhao HL,Fu LJ,Tang GC,et al.Main foodborn pathogens in our country[J].J Med Pest Contrl(医学动物防制),2012,28:1212-1216.
10 CLSI.Methods for dilution antimicrobial susceptibility tests for bacterial that grow aerobically-ninth edition:approved standard M07-A9[S].Wayne PA:Clinical and Laboratory Standards Institute,2012,18-19.
11 Farrell DJ,Sader H S,Rhomberg P R,et al.Gepotidacin(GSK2140944)in vitro activity against Neisseria gonorrhoeae(MIC/MBC,Kill Kinetics,Checkerboard,PAE/SMEtests)[J].Antimicrob.Agents Chemother,2017,61(3):2016-2047.
12 Orhan G,Bayram A,Zer Y,et a1.Synergy tests by E test and chequerboard methods of antimicrobial combinations against Bruce Ua melitensis[J].J Clin Microbiol,2005,43:140-143.
13 Shi DY,Hao YQ,Zhang AR,et al.Progress on cow mastitis caused by Staphylococcus aureus[J].Prog Veter Med(动物医学进展),2010,31:82-86.
14 Zhang P,Yang CX,Chang ZH,et al.Anti-inflammatory effects and mechanisms of traditional Chinese medicine.[J].Prog Veter Med(动物医学进展),2016,37:108-111.
15 Guo MY.The effect and regulation mechanism of Baicalin on the mastitis of Staphylococcus aureus infection in mice[D].Jilin:Jilin University(吉林大学),2014.
16 Dou YQ,Du WL,Xue Y,et al.Study on anti-endotoxin of baicalin[J].West Chin J(华西口腔医学杂志),2007,25:169-172.
17 Tsou LK,Lara-Tejero M,Rosefigura J,et al.Anti-bacterial flavonoids from medicinal plants covalently inactivate type IIIprotein secretion substrates[J].J Am Chem Soc,2016,138:2209-2218.
2 He CN,Peng Y,Xiao W,et al.Comparative study chemical composition in aerial parts and roots of Scutellaria baicalensis[J].Mod Chin Med(中国现代中药),2011,13:32-35.
3 Deng SH,Wang HM,Liu YHStudy on antibacterial activity of scutellaria baicalensis stem-leaf total flavonoid[J].J Chengde Med Coll(承德医学院学报),2008,25:324-325.
4 Zhao TH,Deng SH,Yang HS,et al.Study of antibacterial activity of active fraction from stems and leaves of Scutellaria baicalensis Georgi[J].Chin Pharmacol Bull(中国药理学通报),2007,23:882-886.
5 Shi GX,Shao J,Wang TM,et,al.New advance in studies on antimicrobal activity of Scutellaria baicalensis and its effective ingredients[J].Chin J Chin Mater Med(中国中药),2014,39:3713-3718.
6 Zhang YS,Yang WL,Xiong HP,et al Basic constituent of Ampelopsis grossedentata[J].Nat Prod Res Dev(天然产物研究与开发),2001,13:46-48.
7 Zhou QY,Chen J,Fang JG,et al.Research progress on antibacterial effects of Ampelopsis grossedentata[J].Chin Tradit Herb Drugs(中草药),2017,48:4819-4825.
8 Xu FX,Liu SY,Lan TF,et al.Pollution situation and coping strategies of foodborne pathogens.[J]Food Res Dev(食品研究与开发),2014,1:98-101.
9 Zhao HL,Fu LJ,Tang GC,et al.Main foodborn pathogens in our country[J].J Med Pest Contrl(医学动物防制),2012,28:1212-1216.
10 CLSI.Methods for dilution antimicrobial susceptibility tests for bacterial that grow aerobically-ninth edition:approved standard M07-A9[S].Wayne PA:Clinical and Laboratory Standards Institute,2012,18-19.
11 Farrell DJ,Sader H S,Rhomberg P R,et al.Gepotidacin(GSK2140944)in vitro activity against Neisseria gonorrhoeae(MIC/MBC,Kill Kinetics,Checkerboard,PAE/SMEtests)[J].Antimicrob.Agents Chemother,2017,61(3):2016-2047.
12 Orhan G,Bayram A,Zer Y,et a1.Synergy tests by E test and chequerboard methods of antimicrobial combinations against Bruce Ua melitensis[J].J Clin Microbiol,2005,43:140-143.
13 Shi DY,Hao YQ,Zhang AR,et al.Progress on cow mastitis caused by Staphylococcus aureus[J].Prog Veter Med(动物医学进展),2010,31:82-86.
14 Zhang P,Yang CX,Chang ZH,et al.Anti-inflammatory effects and mechanisms of traditional Chinese medicine.[J].Prog Veter Med(动物医学进展),2016,37:108-111.
15 Guo MY.The effect and regulation mechanism of Baicalin on the mastitis of Staphylococcus aureus infection in mice[D].Jilin:Jilin University(吉林大学),2014.
16 Dou YQ,Du WL,Xue Y,et al.Study on anti-endotoxin of baicalin[J].West Chin J(华西口腔医学杂志),2007,25:169-172.
17 Tsou LK,Lara-Tejero M,Rosefigura J,et al.Anti-bacterial flavonoids from medicinal plants covalently inactivate type IIIprotein secretion substrates[J].J Am Chem Soc,2016,138:2209-2218.