氨氮和亚硝酸氮对偏顶蛤的急性毒性研究
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摘要
[目的]研究氨氮和亚硝酸氮对偏顶蛤的急性毒性,为合理调控偏顶蛤水体中氨氮与亚硝酸氮提供参考价值。[方法]采用常规生物毒性试验方法,研究偏顶蛤对氨氮和亚硝酸氮毒性的耐受力。[结果]氨氮对偏顶蛤的半致死浓度96 h LC50为158.85 mg/L,安全浓度(SC)为15.89 mg/L;非离子氨对偏顶蛤半致死浓度96 h LC50为14.06 mg/L,安全浓度(SC)为1.41 mg/L。亚硝酸氮对试验蛤的半致死浓度96 h LC50为54.20 mg/L,安全浓度(SC)为5.42 mg/L。[结论]与亚硝酸盐相比,偏顶蛤对总氨氮毒性的耐受力更强,非离子氨对偏顶蛤的毒性最强。
[Objective] To research the acute toxicity of ammonia nitrogen and nitrite to Modiolus modiolus,and to provide references for the rational adjustment of ammonia nitrogen and nitrite nitrogen in M. modiolus. [Method] Conventional biological toxicity test was used to research the tolerance of M. modiolus to ammonia nitrogen and nitrite. [Result]The 96 h LC50 of total ammonia nitrogen,non-ionic ammonia nitrogen and nitrite nitrogen to M. modiolus were 158. 85,14. 06 and 54. 20 mg / L,respectively. And the safe concentrations( SC) of total ammonia nitrogen,non-ionic ammonia nitrogen and nitrite nitrogen to the mussels were 15. 89,1. 41 and 5. 42 mg / L,respectively. [Conclusion] M. modiolus has stronger tolerance to total ammonia nitrogen toxicity than nitrite nitrogen,and the high toxicity occurs in non-ionic ammonia nitrogen.
[Objective] To research the acute toxicity of ammonia nitrogen and nitrite to Modiolus modiolus,and to provide references for the rational adjustment of ammonia nitrogen and nitrite nitrogen in M. modiolus. [Method] Conventional biological toxicity test was used to research the tolerance of M. modiolus to ammonia nitrogen and nitrite. [Result]The 96 h LC50 of total ammonia nitrogen,non-ionic ammonia nitrogen and nitrite nitrogen to M. modiolus were 158. 85,14. 06 and 54. 20 mg / L,respectively. And the safe concentrations( SC) of total ammonia nitrogen,non-ionic ammonia nitrogen and nitrite nitrogen to the mussels were 15. 89,1. 41 and 5. 42 mg / L,respectively. [Conclusion] M. modiolus has stronger tolerance to total ammonia nitrogen toxicity than nitrite nitrogen,and the high toxicity occurs in non-ionic ammonia nitrogen.
引文
[1]齐钟彦,马绣同,王祯瑞,等.黄渤海的软体动物[M].北京:农业出版社,1987:164-166.
[2]宁军号,宋坚,常亚青,等.偏顶蛤性腺发育和生殖周期[J].中国水产科学,2015,22(3):469-477.
[3]宁军号,胡盼,宋坚,等.几种环境因子对偏顶蛤浮游幼虫生长和发育的影响[J].大连海洋大学学报,2014,29(6):601-606.
[4]常亚青.贝类增养殖学[M].北京:中国农业出版社,2007:65-71.
[3]雷衍之.养殖水环境化学[M].北京:中国农业出版社,2004:11-13.
[4]王如才,王昭萍,张建中.海水贝类养殖学[M].青岛:青岛海洋大学出版社,2003:4-6.
[5]刘媛,王建,孙建峰,等.我国海洋贝类资源的利用现状和发展趋势[J].现代食品科技,2013,29(3):673-676.
[6]吕富,黄金田,吕林兰,等.不同盐度条件下氨氮对双齿围沙蚕的毒性研究[J].安徽农业科学,2009,37(7):2986-2987,2994.
[7]孙舰军,丁美丽.氨氮对中国对虾抗病力的影响[J].海洋与湖沼,1999,30(3):267-272.
[8]SPRAGUE J B.Measurement of pollutant toxicity of fish III.Sublethal effects and safe concentrations[J].Water research,1971,5:245-266.
[9]王清印.海水养殖与碳汇渔业[M].北京:海洋出版社,2012:527-530.
[10]常亚青.贝类增养殖学[M].北京:中国农业出版社,2007:72-74.
[11]白秀娟,冯波,卢伙胜,等.亚硝酸盐对文昌鱼的急性毒性效应[J].安徽农业科学,2013,41(20):8522-8524.
[12]ARMSTRONG D A,CHIPPENDALE D,KNIGHT A W.Interaction of ionized and un-ionized ammonia on short-term survival and growth of prawn larvae,Macrobrachium rosenbergii[J].Biological bulletin,1978,154:15-31.
[13]ALDERSON R.The effect of ammonia on the growth of juvenile dover sole,Sole sole(L.)and turbot,Scophthalmus maximus(L.)[J].Aquaculture,1979,17(4):291-309.
[14]PERSON L E,RUYET J,CHARTOIS H,et al.Comparative acute ammonia toxicity in marine fish and plasma ammonia response[J].Aquaculture,1995,136(1):181-194.
[15]潘小玲,陈百悦,樊海平.非离子氨及亚硝酸盐对欧洲鳗鲡的急性毒性试验[J].水产科技情报,1998,25(1):20-23.
[16]CHENG S Y,CHEN J C.Haemocyanin oxygen affinity and the fractionation of oxyhem ocyanin and deoxy hemocy anin for Penaeus monodom exposed to elevated nitrite[J].Aquat toxicol,1999,45:35-46.
[17]吕永林,张永普,单赛赛,等.不同温度条件下氨氮对泥蚶的急性毒性影响[J].浙江海洋学院学报,2012,31(1):55-64.
[18]王娟,曲克明,刘海英,等.不同溶氧条件下亚硝酸盐和氨氮对中国对虾的急性毒性效应[J].海洋水产研究,2007,28(6):1-6.
[19]赖晓芳,沈善瑞,吕海波.氨态氮和p H对脊尾白虾氮磷代谢的影响[J].安徽农业科学,2013,41(13):5745-5746.
[20]师尚丽,马奕成,郑莲,等.不同p H和盐度下氨氮对方斑东风螺的毒性研究[J].湛江海洋大学学报,2005,25(6):36-40.
[21]CHEN J C,NAN F H.Changes of oxygen consumption and ammonia-N excretion by Penaeus chinensis Osbeck at different temperature and salinity levels[J].Journal of crustacean biology,1993(4):706-712.
[22]郭增华,王秋燕.亚硝酸盐氮对方斑东风螺毒性的研究[J].海洋水产研究,27(6):88-92.
[23]黄翔鹄,李长玲,郑莲,等.亚硝酸盐氮对凡纳滨对虾毒性和抗病相关因子影响[J].水生生物学报,2006,30(4):466-471,907
[24]曲克明,徐勇,马绍赛,等.不同溶氧条件下亚硝酸盐和非离子氨对大菱鲆的急性毒性效应[J].海洋水产研究,2007,28(4):83-88.
[2]宁军号,宋坚,常亚青,等.偏顶蛤性腺发育和生殖周期[J].中国水产科学,2015,22(3):469-477.
[3]宁军号,胡盼,宋坚,等.几种环境因子对偏顶蛤浮游幼虫生长和发育的影响[J].大连海洋大学学报,2014,29(6):601-606.
[4]常亚青.贝类增养殖学[M].北京:中国农业出版社,2007:65-71.
[3]雷衍之.养殖水环境化学[M].北京:中国农业出版社,2004:11-13.
[4]王如才,王昭萍,张建中.海水贝类养殖学[M].青岛:青岛海洋大学出版社,2003:4-6.
[5]刘媛,王建,孙建峰,等.我国海洋贝类资源的利用现状和发展趋势[J].现代食品科技,2013,29(3):673-676.
[6]吕富,黄金田,吕林兰,等.不同盐度条件下氨氮对双齿围沙蚕的毒性研究[J].安徽农业科学,2009,37(7):2986-2987,2994.
[7]孙舰军,丁美丽.氨氮对中国对虾抗病力的影响[J].海洋与湖沼,1999,30(3):267-272.
[8]SPRAGUE J B.Measurement of pollutant toxicity of fish III.Sublethal effects and safe concentrations[J].Water research,1971,5:245-266.
[9]王清印.海水养殖与碳汇渔业[M].北京:海洋出版社,2012:527-530.
[10]常亚青.贝类增养殖学[M].北京:中国农业出版社,2007:72-74.
[11]白秀娟,冯波,卢伙胜,等.亚硝酸盐对文昌鱼的急性毒性效应[J].安徽农业科学,2013,41(20):8522-8524.
[12]ARMSTRONG D A,CHIPPENDALE D,KNIGHT A W.Interaction of ionized and un-ionized ammonia on short-term survival and growth of prawn larvae,Macrobrachium rosenbergii[J].Biological bulletin,1978,154:15-31.
[13]ALDERSON R.The effect of ammonia on the growth of juvenile dover sole,Sole sole(L.)and turbot,Scophthalmus maximus(L.)[J].Aquaculture,1979,17(4):291-309.
[14]PERSON L E,RUYET J,CHARTOIS H,et al.Comparative acute ammonia toxicity in marine fish and plasma ammonia response[J].Aquaculture,1995,136(1):181-194.
[15]潘小玲,陈百悦,樊海平.非离子氨及亚硝酸盐对欧洲鳗鲡的急性毒性试验[J].水产科技情报,1998,25(1):20-23.
[16]CHENG S Y,CHEN J C.Haemocyanin oxygen affinity and the fractionation of oxyhem ocyanin and deoxy hemocy anin for Penaeus monodom exposed to elevated nitrite[J].Aquat toxicol,1999,45:35-46.
[17]吕永林,张永普,单赛赛,等.不同温度条件下氨氮对泥蚶的急性毒性影响[J].浙江海洋学院学报,2012,31(1):55-64.
[18]王娟,曲克明,刘海英,等.不同溶氧条件下亚硝酸盐和氨氮对中国对虾的急性毒性效应[J].海洋水产研究,2007,28(6):1-6.
[19]赖晓芳,沈善瑞,吕海波.氨态氮和p H对脊尾白虾氮磷代谢的影响[J].安徽农业科学,2013,41(13):5745-5746.
[20]师尚丽,马奕成,郑莲,等.不同p H和盐度下氨氮对方斑东风螺的毒性研究[J].湛江海洋大学学报,2005,25(6):36-40.
[21]CHEN J C,NAN F H.Changes of oxygen consumption and ammonia-N excretion by Penaeus chinensis Osbeck at different temperature and salinity levels[J].Journal of crustacean biology,1993(4):706-712.
[22]郭增华,王秋燕.亚硝酸盐氮对方斑东风螺毒性的研究[J].海洋水产研究,27(6):88-92.
[23]黄翔鹄,李长玲,郑莲,等.亚硝酸盐氮对凡纳滨对虾毒性和抗病相关因子影响[J].水生生物学报,2006,30(4):466-471,907
[24]曲克明,徐勇,马绍赛,等.不同溶氧条件下亚硝酸盐和非离子氨对大菱鲆的急性毒性效应[J].海洋水产研究,2007,28(4):83-88.