热休克蛋白27在病毒感染中的作用研究进展
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摘要
热休克蛋白27(Hsp27)是一种结构上高度保守的小热休克应激蛋白,具有多重生物学功能,在调节蛋白质稳态以维持细胞骨架有至关重要的作用。近年来,热休克蛋白(HSPs)在疾病预防和药物开发中取得较大突破,有研究发现在许多病毒感染宿主细胞后,细胞中Hsp27的表达会发生显著变化,表明Hsp27与病毒感染之间有密切关联,但Hsp27在病毒感染中的应用尚不成熟。论文主要介绍病毒感染宿主细胞后,Hsp27通过抑制细胞的自噬、凋亡、干扰素形成及维持病毒复制等发挥其在病毒感染中作用,此方面研究为以HSPs为靶点研发抗病毒药物提供了新思路。
Heat shock protein 27(Hsp27)is a highly conserved small heat shock protein with multiple biological functions,and it plays a vital role in regulating protein homeostasis to maintain the cytoskeleton.In recent years,HSPs have made major breakthroughs in disease prevention and drug development,studies have shown that expression of Hsp27 significantly changes in host cells after infection with many viruses,which indicate that Hsp27 is closely related to viral infection.However,the application and exploration of Hsp27 in viral infection is not yet mature.The review summarized the roles of HSP27 in viral infection through inhibiting the autophagy,apoptosis,interferon formation and the maintaining the virus replication in infected cells.These studies provide new ideas for the development of antiviral drugs using HSPs as targets and provide reference for further research of Hsp27.
Heat shock protein 27(Hsp27)is a highly conserved small heat shock protein with multiple biological functions,and it plays a vital role in regulating protein homeostasis to maintain the cytoskeleton.In recent years,HSPs have made major breakthroughs in disease prevention and drug development,studies have shown that expression of Hsp27 significantly changes in host cells after infection with many viruses,which indicate that Hsp27 is closely related to viral infection.However,the application and exploration of Hsp27 in viral infection is not yet mature.The review summarized the roles of HSP27 in viral infection through inhibiting the autophagy,apoptosis,interferon formation and the maintaining the virus replication in infected cells.These studies provide new ideas for the development of antiviral drugs using HSPs as targets and provide reference for further research of Hsp27.
引文
[1]吴燕,王琦,周碧君,等.热休克蛋白免疫增效作用研究进展[J].动物医学进展,2015,36(8):82-85.
[2]Liu Y,Zhang X.Heat shock protein reports on proteome stress[J].Biotechnol J,2018,13(4):doi:10.1002/biot.201800039.
[3]Wang H C,Yen T C,Huang L R.Method using heat shock protein inhibitor for treating hepatitis and hepatoma[P].:US9687499,2017-06-27.
[4]Kolinski T,Marek-Trzonkowska N,Trzonkowski P,et al.Heat shock proteins(HSPs)in the homeostasis of regulatory T cells(Tregs)[J].Cent Eur J Immunol,2016,41(3):317-323.
[5]Jovcevski B,Kelly M A,Rote A P,et al.Phosphomimics destabilize Hsp27oligomeric assemblies and enhance chaperone activity[J].Chem Biol,2015,22(2):186-195.
[6]Zou S L,Liao M F,Yang J L,et al.Heat shock protein 27plays aprotective role in thoracic aortic dissection by promoting cell proliferation and inhibiting apoptosis.[J].Cell Mol Biol Lett,2017,22(1):24-39.
[7]张宝宏,谢飞,任海军,等.HSP27对细胞迁移的调控[J].生物化学与生物物理进展,2017,44(2):110-116.
[8]Arrigo A P.Analysis of HspB1(Hsp27)oligomerization and phosphorylation patterns and its interaction with specific client polypeptides[J].Methods Mol Biol,2018,1709:163-178.
[9]Dokladny K,Myers O B,Moseley P L.Heat shock response and autophagy—cooperation and control[J].Autophagy,2015,11(2):200-213.
[10]Dokladny K,Zuhl M N,Mandell M,et al.Regulatory coordination between two major intracellular homeostatic systems:heat shock response and autophagy[J].J Biol Chem,2013,288(21):14959-14972.
[11]Li M,Li J,Zeng R H,et al.Respiratory syncytial virus replication is promoted by autophagy-mediated inhibition of apoptosis[J].J Virol,2018,92(8):e02193-17.
[12]尹彩霞,于少雄,仇华吉.细胞受体介导的疱疹病毒侵入机制[J].微生物学报,2018,58(1):1-9.
[13]陈炅然,严作廷,尚若峰,等.抗病毒免疫中宿主细胞的自溶作用以及病毒的进化[J].动物医学进展,2010,31(12):132-137.
[14]杨帅,刘璐,宋迪,等.细胞自噬与病毒感染[J].新乡医学院学报,2017,34(1):1-3.
[15]徐利军,刘秀梵,顾敏.自噬与A型流感病毒感染[J].动物医学进展,2017,38(1):94-99.
[16]Tanaka T,Iino M,Goto K.Sec6enhances cell migration and suppresses apoptosis by elevating the phosphorylation of p38MAPK,MK2,and HSP27[J].Cell Signal,2018,49:1-16.
[17]Rajaiya J,Yousuf M A,Singh G,et al.Heat shock protein 27mediated signaling in viral infection[J].Biochemistry,2012,51(28):5695-5702.
[18]曹宇航,王斐,刘晓云,等.HSP27和Annexin A2蛋白对PRRSV感染的影响[J].中国兽医学报,2014,34(4):618-621.
[19]Nahomi R B,Dimauro M A,Wang B,et al.Identification of peptides in human Hsp20and Hsp27that possess molecular chaperone and anti-apoptotic activities[J].Biochem J,2015,465(1):115-125.
[20]Hayashi N,Peacock J W,Beraldi E,et al.Hsp27silencing coordinately inhibits proliferation and promotes Fas-induced apoptosis by regulating the PEA-15 molecular switch[J].Cell Death Differ,2012,19(6):990-1002.
[21]周长平,陈莹,李媛,等.鸡毒支原体热休克蛋白GrpE粘附特性的鉴定[J].中国预防兽医学报,2017,39(1):28-33.
[22]Mathew S S,Della S M,Burch A D.Modification and reorganization of the cytoprotective cellular chaperone Hsp27during herpes simplex virus type 1infection[J].J Virol,2009,83(18):9304-9312.
[23]Ling S,Luo M,Jiang S,et al.Cellular Hsp27interacts with classical swine fever virus NS5Aprotein and negatively regulates viral replication by the NF-kappaB signaling pathway[J].Virology,2018,518:202-209.
[24]甘霖,赵俊,陈敬贤,等.干扰素抗病毒效力影响因素的研究进展[J].微生物与感染,2018,13(1):43-48.
[25]Milani A,Bolhassani A,Shahbazi S,et al.Small heat shock protein 27:An effective adjuvant for enhancement of HIV-1Nef antigen-specific immunity[J].Immunol Lett,2017,191:16-22.
[26]孟镇锴,王洪宝,董延生,等.热休克蛋白抑制剂抗甲型流感病毒研究概况[J].中国新药杂志,2018,27(8):882-886.
[27]Le Y,Jia P,Jin Y,et al.The antiviral role of heat shock protein 27against red spotted grouper nervous necrosis virus infection in sea perch[J].Fish Shellfish Immunol,2017,70:185-194.
[28]李铮,刘晓玲,赵振东,等.热休克蛋白27增强A型流感病毒NS1对β干扰素的抑制作用[J].生物工程学报,2012,28(10):1205-1215.
[2]Liu Y,Zhang X.Heat shock protein reports on proteome stress[J].Biotechnol J,2018,13(4):doi:10.1002/biot.201800039.
[3]Wang H C,Yen T C,Huang L R.Method using heat shock protein inhibitor for treating hepatitis and hepatoma[P].:US9687499,2017-06-27.
[4]Kolinski T,Marek-Trzonkowska N,Trzonkowski P,et al.Heat shock proteins(HSPs)in the homeostasis of regulatory T cells(Tregs)[J].Cent Eur J Immunol,2016,41(3):317-323.
[5]Jovcevski B,Kelly M A,Rote A P,et al.Phosphomimics destabilize Hsp27oligomeric assemblies and enhance chaperone activity[J].Chem Biol,2015,22(2):186-195.
[6]Zou S L,Liao M F,Yang J L,et al.Heat shock protein 27plays aprotective role in thoracic aortic dissection by promoting cell proliferation and inhibiting apoptosis.[J].Cell Mol Biol Lett,2017,22(1):24-39.
[7]张宝宏,谢飞,任海军,等.HSP27对细胞迁移的调控[J].生物化学与生物物理进展,2017,44(2):110-116.
[8]Arrigo A P.Analysis of HspB1(Hsp27)oligomerization and phosphorylation patterns and its interaction with specific client polypeptides[J].Methods Mol Biol,2018,1709:163-178.
[9]Dokladny K,Myers O B,Moseley P L.Heat shock response and autophagy—cooperation and control[J].Autophagy,2015,11(2):200-213.
[10]Dokladny K,Zuhl M N,Mandell M,et al.Regulatory coordination between two major intracellular homeostatic systems:heat shock response and autophagy[J].J Biol Chem,2013,288(21):14959-14972.
[11]Li M,Li J,Zeng R H,et al.Respiratory syncytial virus replication is promoted by autophagy-mediated inhibition of apoptosis[J].J Virol,2018,92(8):e02193-17.
[12]尹彩霞,于少雄,仇华吉.细胞受体介导的疱疹病毒侵入机制[J].微生物学报,2018,58(1):1-9.
[13]陈炅然,严作廷,尚若峰,等.抗病毒免疫中宿主细胞的自溶作用以及病毒的进化[J].动物医学进展,2010,31(12):132-137.
[14]杨帅,刘璐,宋迪,等.细胞自噬与病毒感染[J].新乡医学院学报,2017,34(1):1-3.
[15]徐利军,刘秀梵,顾敏.自噬与A型流感病毒感染[J].动物医学进展,2017,38(1):94-99.
[16]Tanaka T,Iino M,Goto K.Sec6enhances cell migration and suppresses apoptosis by elevating the phosphorylation of p38MAPK,MK2,and HSP27[J].Cell Signal,2018,49:1-16.
[17]Rajaiya J,Yousuf M A,Singh G,et al.Heat shock protein 27mediated signaling in viral infection[J].Biochemistry,2012,51(28):5695-5702.
[18]曹宇航,王斐,刘晓云,等.HSP27和Annexin A2蛋白对PRRSV感染的影响[J].中国兽医学报,2014,34(4):618-621.
[19]Nahomi R B,Dimauro M A,Wang B,et al.Identification of peptides in human Hsp20and Hsp27that possess molecular chaperone and anti-apoptotic activities[J].Biochem J,2015,465(1):115-125.
[20]Hayashi N,Peacock J W,Beraldi E,et al.Hsp27silencing coordinately inhibits proliferation and promotes Fas-induced apoptosis by regulating the PEA-15 molecular switch[J].Cell Death Differ,2012,19(6):990-1002.
[21]周长平,陈莹,李媛,等.鸡毒支原体热休克蛋白GrpE粘附特性的鉴定[J].中国预防兽医学报,2017,39(1):28-33.
[22]Mathew S S,Della S M,Burch A D.Modification and reorganization of the cytoprotective cellular chaperone Hsp27during herpes simplex virus type 1infection[J].J Virol,2009,83(18):9304-9312.
[23]Ling S,Luo M,Jiang S,et al.Cellular Hsp27interacts with classical swine fever virus NS5Aprotein and negatively regulates viral replication by the NF-kappaB signaling pathway[J].Virology,2018,518:202-209.
[24]甘霖,赵俊,陈敬贤,等.干扰素抗病毒效力影响因素的研究进展[J].微生物与感染,2018,13(1):43-48.
[25]Milani A,Bolhassani A,Shahbazi S,et al.Small heat shock protein 27:An effective adjuvant for enhancement of HIV-1Nef antigen-specific immunity[J].Immunol Lett,2017,191:16-22.
[26]孟镇锴,王洪宝,董延生,等.热休克蛋白抑制剂抗甲型流感病毒研究概况[J].中国新药杂志,2018,27(8):882-886.
[27]Le Y,Jia P,Jin Y,et al.The antiviral role of heat shock protein 27against red spotted grouper nervous necrosis virus infection in sea perch[J].Fish Shellfish Immunol,2017,70:185-194.
[28]李铮,刘晓玲,赵振东,等.热休克蛋白27增强A型流感病毒NS1对β干扰素的抑制作用[J].生物工程学报,2012,28(10):1205-1215.