大蒜秸秆可溶性膳食纤维的超声复合酶解提取及其抗氧化性分析
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摘要
为了增强大蒜秸秆膳食纤维的生理功能,研究了不同提取方法对其可溶性膳食纤维(Soluble dietary fiber,SDF)含量的影响。以SDF含量为响应值,对比分析超声、酶解及超声复合酶解法的改性效果,研究固液比、超声时间、超声功率、加酶量和酶解时间等因素对SDF得率的影响,通过羟基(·OH)和1,1-二苯基-2-苦基肼自由基(DPPH·)清除率评价SDF的体外抗氧化能力。结果表明,超声复合酶解法提取的SDF得率最高,通过单因素优化试验确定其最佳提取条件为固液比1∶30、超声功率300 W、超声时间15 min、加酶量5%、酶解时间4 h;SDF对·OH和DPPH·的清除率较强,其IC50(50%Inhibitory concentration,IC50)分别为1.41和0.64 mg/mL,且在一定浓度范围内,自由基清除率与SDF浓度呈现剂量效应线性关系,说明大蒜秸秆SDF是一种较好的抗氧化剂。
In order to enhance the physiological function of garlic straw dietary fiber,the different extraction methods on the soluble dietary fiber(Soluble dietary fiber,SDF) content were studied. Taking SDF content as the response value,the modification effects of ultrasound,enzymatic hydrolysis and ultrasonic complex enzymatic hydrolysis were compared and analyzed,and the effects of solid-liquid ratio,ultrasound time,ultrasound power,enzyme amount and enzymatic hydrolysis time on the yield of SDF were studied. The antioxidant capacity of SDF in vitro was evaluated by the clearance rate of hydroxyl(·OH) and 1,1-diphenyl-2-amylhydrazine free radicals(DPPH·). The results suggested that when garlic straw was pretreated by ultrasonic assisted enzymatic hydrolysis method,the SDF yield was the highest. The optimal extraction conditions consisted of solid/liquid ratio of 1∶30, ultrasonic power of 300 W,ultrasonic time of 15 min,E/S ration of 5% and enzymatic hydrolysis of 4 h. The IC50 of SDF on OH· and DPPH· were obtained to be 1.41 and 0.64 mg/m L, respectively. Moreover,the relationship between free radical scavenging rate and SDF concentration was linear, suggesting that SDF was a good antioxidant.
In order to enhance the physiological function of garlic straw dietary fiber,the different extraction methods on the soluble dietary fiber(Soluble dietary fiber,SDF) content were studied. Taking SDF content as the response value,the modification effects of ultrasound,enzymatic hydrolysis and ultrasonic complex enzymatic hydrolysis were compared and analyzed,and the effects of solid-liquid ratio,ultrasound time,ultrasound power,enzyme amount and enzymatic hydrolysis time on the yield of SDF were studied. The antioxidant capacity of SDF in vitro was evaluated by the clearance rate of hydroxyl(·OH) and 1,1-diphenyl-2-amylhydrazine free radicals(DPPH·). The results suggested that when garlic straw was pretreated by ultrasonic assisted enzymatic hydrolysis method,the SDF yield was the highest. The optimal extraction conditions consisted of solid/liquid ratio of 1∶30, ultrasonic power of 300 W,ultrasonic time of 15 min,E/S ration of 5% and enzymatic hydrolysis of 4 h. The IC50 of SDF on OH· and DPPH· were obtained to be 1.41 and 0.64 mg/m L, respectively. Moreover,the relationship between free radical scavenging rate and SDF concentration was linear, suggesting that SDF was a good antioxidant.
引文
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[2]曲玲玉,李大为,张鹏,等.酶水解制备山药皮可溶性膳食纤维及性能测定[J].天然产物研究与开发,2015,27(3):496-501,511.
[3] CASTELLANOS-JANKIEWICZ A,BOSQUE-PLATA L D,TEJERO M E. Combined effect of plant sterols and dietary fiber for the treatment of hypercholesterolemia[J]. Plant foods for human nutrition,2014,69(2):93-100.
[4] AL-SAYED H M A,AHMED A R. Utilization of watermelon rinds and sharlyn melon peels as a natural source of dietary fiber and antioxidants in cake[J].Annals of agricultural sciences,2013,58(1):83-95.
[5]余旭东,刘倩,许秀芳,等.超声促进两组分多彩发光体系分子聚集体的共组装及其对酸的响应行为[J].化学学报,2012,70(19):2016-2023.
[6] CHEN S N,JIANG L Z,LI Y,et al. Ultrasound-assisted enzymatic extraction of dietary fiber from pods[J].Procedia engineering,2011,15:5056-5061.
[7] LIAO N B,ZHONG J J,YE X Q,et al. Ultrasonic-assisted enzymatic extraction of polysaccharide from Corbicula fluminea:Characterization and antioxidant activity[J].LWT-food science and technology,2015,60(2):1113-1121.
[8] JIN J,MA H L,WANG W W,et al. Effects and mechanism of ultrasound pretreatment on rapeseed protein enzymolysis[J].Journal of the science of food and agriculture,2016,96(4):1159-1166.
[9] WANG D Y,ZHAO Y,JIAO Y D,et al. Antioxidative and hepatoprotective effects of the polysaccharides from Zizyphus ujube cv. Shaanbeitanzao[J].Carbohydrate polymers,2012,88(4):1453-1459.
[10] HUANG S J,MAU J L. Antioxidant properties of methanolic extracts from Agaricus blazei with various doses ofγ-irradiation[J].LWT-food science and technology,2006,39(7):707-716.
[11] CHENG Y,LIU Y,WU J,et al. Improving the enzymolysis efficiency of potato protein by simultaneous dual-frequency energy-gathered ultrasound pretreatment:Thermodynamics and kinetics[J].Ultrasonics sonochemistry,2017,37:351-359.
[12]赖爱萍,陆国权,王颖.超声波辅助酶法制备甘薯渣膳食纤维工艺研究[J].中国粮油学报,2015,30(8):99-104.
[13] HOCHESTEIN P,ATALLAH A S. The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer[J].Mutation research,1988,202(2):363-375.
[14] EG譈魪S I,SANCHEZ C,MONDRAGON I,et al. Antioxidant activity of phenolic compounds obtained by autohydrolysis of corn residues[J].Industrial crops and products,2012,36:164-171.