南瓜未授粉子房培养形成再生植株的研究
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摘要
以‘贵阳小青瓜’(南瓜)未授粉子房为外植体,在离体条件下研究热激时间和TDZ浓度对胚状体诱导的影响。进一步优化试验步骤,探讨直接获得形成再生植株的方法,加速育种进程。结果表明:35℃高温黑暗培养5d后转入25℃光照培养90d能直接形成再生植株;培养基中附加TDZ浓度在0.04~0.06mg/L能诱导离体子房直接产生再生植株,并以0.06mg/L诱导效果最好,出胚率为22.57%,植株再生率为14.30%。
We used unfertilized ovaries of‘Guiyang xiaoqinggua'(pumpkin)as the explants to study the heat shock time and TDZ concentration on embryo induction via in vitro culture.Furthermore,we optimized the test steps and researched the method of obtaining regenerated plants directly.The results showed that the best obtained pumpkin plants were inducted regenerated plants directly under dark at 35℃for 5 d,and then moved to light condition at 25℃for 90 d;The unfertilized ovaries can induct regenerated plants in the TDZ concentration at 0.04-0.06 mg/L,the best TDZ concentration was 0.06 mg/L,and the ratio of inducted embryo and regenerated plants were 22.57% and 14.30%,respectively.
We used unfertilized ovaries of‘Guiyang xiaoqinggua'(pumpkin)as the explants to study the heat shock time and TDZ concentration on embryo induction via in vitro culture.Furthermore,we optimized the test steps and researched the method of obtaining regenerated plants directly.The results showed that the best obtained pumpkin plants were inducted regenerated plants directly under dark at 35℃for 5 d,and then moved to light condition at 25℃for 90 d;The unfertilized ovaries can induct regenerated plants in the TDZ concentration at 0.04-0.06 mg/L,the best TDZ concentration was 0.06 mg/L,and the ratio of inducted embryo and regenerated plants were 22.57% and 14.30%,respectively.
引文
[1]ADAMS GG,IMRAN S,WANG S,et al.The hypo-glycemic effect of pumpkin seeds,trigonelline(TRG),nicotinic acid(NA),and D-chiro-inositol(DCI)in control-ling glycemic levels in diabetes mellitus[J].Critical Reviews in Food Science&Nutrition,2014,54:1 322-1 329.
[2]张伶俐,崔崇士,屈淑平.植物离体雌核发育的研究进展[J].东北农业大学学报,2009,40(11):133-136.ZHANG L L,CUI C S,QU S P.Progress of plants in vitro gynogenesis[J].Journal of Northeast Agricultural University,2009,40(11):133-136.
[3]BASU S K,DATTA M,SHARMA M,et al.Haploid production technology in wheat and some selected higher plants[J].Australian Journal of Crop Science,2011,5:1 087-1 093.
[4]雷春,陈劲枫.葫芦科蔬菜作物单倍体材料创制的研究进展[J].中国蔬菜,2006,1(1):33-36.LEI C,CHEN J F.Advances in haploid material creation in vegetable crops of Cucurbit[J].Chinese Vegetables,2006,1(1):33-36.
[5]CHEN J F,CUI L,MALIK A A,et al.In vitro haploid and dihaploid production via unfertilized ovule culture[J].Plant Cell Tissue&Organ Culture,2011,104:311-319.
[6]徐静.西葫芦雌核离体高效培养体系的建立[D].乌鲁木齐:新疆农业大学,2007.
[7]韩丽华,王建设,陈贵林.AgNO3对甜瓜离体胚囊发育的影响[J].河北农业大学学报,2005,28(2):48-50.HAN L H,WANG J S,CHEN G L.The effects of AgNO3on vitro gynogenesis induction in melon[J].Journal of Agricultural University of Hebei,2005,28(2):48-50.
[8]GEMES-JUH U'SZ A,BALOGH P,FERENCZY A,et al.Effect of optimal stage of female game tophyte and heat treatment on in vitro gynogenesis induction in cucumber(Cucumis sativus L.)[J].Plant Cell Reports,2002,21:105-111.
[9]孙守如,章鹏,胡建斌,等.南瓜未受精胚珠的离体培养及植株再生[J].植物学报,2013,48(1):79-86.SUN S R,ZHANG P,HU J B,et al.In vitro culture of punpkin unfertilized ovules and plant regeneration[J].Chinese Bulletin of Botany,2013,48(1):79-86.
[10]闵子扬,李涵,邹甜,等.南瓜未授粉子房离体培养及植株再生[J].植物学报,2016,51(1):74-80.MIN Z Y,LI H,ZOU T,et al.Studies of in vitro culture and plant regeneration of unfertilized ovary of pumpkin[J].Chinese Bulletin of Botany,2016,51(1):74-80.
[11]翟庆慧.南瓜未受精子房和未受精胚珠离体培养研究[D].郑州:河南农业大学,2009.
[12]孙守如,翟庆慧,胡建斌,等.几种生理因素对子房未授粉南瓜胚形成的影响[J].植物生理学通讯,2009,45(10):977-980.SUN S R,ZHAI Q H,HU J B,et al.Effects of several physiological factors on embryo formation in unpollinated ovary culture of pumpkin[J].Plant Physiology Communication,2009,45(10):977-980.
[13]徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂[J].植物学通报,2003,20(2):227-237.XU X F,HUANG X L.TDZ:an efficacious plant growth regulator[J].Chinese Bulletin of Botany,2003,20(2):227-237.
[14]OUEDRAOGO J T,ST-PIERRE C A,COLLIN J,et al.Effect of amino acids grow regulators and genotype on androgenesis in barley[J].Plant Cell Tissue&Organ Culture,1998,53:59-66.
[15]刁卫平,陈劲枫,雷春,等.影响黄瓜未授粉子房培养胚发生因素的研究[J].南京农业大学学报,2008,31(1):137-140.DIAO W P,CHEN J F,LEI C,et al.Factors affecting embryo formation in unpollinated ovary culture of cucumber[J].Journal of Nanjing Agricultural University,2008,31(1):137-140.
[2]张伶俐,崔崇士,屈淑平.植物离体雌核发育的研究进展[J].东北农业大学学报,2009,40(11):133-136.ZHANG L L,CUI C S,QU S P.Progress of plants in vitro gynogenesis[J].Journal of Northeast Agricultural University,2009,40(11):133-136.
[3]BASU S K,DATTA M,SHARMA M,et al.Haploid production technology in wheat and some selected higher plants[J].Australian Journal of Crop Science,2011,5:1 087-1 093.
[4]雷春,陈劲枫.葫芦科蔬菜作物单倍体材料创制的研究进展[J].中国蔬菜,2006,1(1):33-36.LEI C,CHEN J F.Advances in haploid material creation in vegetable crops of Cucurbit[J].Chinese Vegetables,2006,1(1):33-36.
[5]CHEN J F,CUI L,MALIK A A,et al.In vitro haploid and dihaploid production via unfertilized ovule culture[J].Plant Cell Tissue&Organ Culture,2011,104:311-319.
[6]徐静.西葫芦雌核离体高效培养体系的建立[D].乌鲁木齐:新疆农业大学,2007.
[7]韩丽华,王建设,陈贵林.AgNO3对甜瓜离体胚囊发育的影响[J].河北农业大学学报,2005,28(2):48-50.HAN L H,WANG J S,CHEN G L.The effects of AgNO3on vitro gynogenesis induction in melon[J].Journal of Agricultural University of Hebei,2005,28(2):48-50.
[8]GEMES-JUH U'SZ A,BALOGH P,FERENCZY A,et al.Effect of optimal stage of female game tophyte and heat treatment on in vitro gynogenesis induction in cucumber(Cucumis sativus L.)[J].Plant Cell Reports,2002,21:105-111.
[9]孙守如,章鹏,胡建斌,等.南瓜未受精胚珠的离体培养及植株再生[J].植物学报,2013,48(1):79-86.SUN S R,ZHANG P,HU J B,et al.In vitro culture of punpkin unfertilized ovules and plant regeneration[J].Chinese Bulletin of Botany,2013,48(1):79-86.
[10]闵子扬,李涵,邹甜,等.南瓜未授粉子房离体培养及植株再生[J].植物学报,2016,51(1):74-80.MIN Z Y,LI H,ZOU T,et al.Studies of in vitro culture and plant regeneration of unfertilized ovary of pumpkin[J].Chinese Bulletin of Botany,2016,51(1):74-80.
[11]翟庆慧.南瓜未受精子房和未受精胚珠离体培养研究[D].郑州:河南农业大学,2009.
[12]孙守如,翟庆慧,胡建斌,等.几种生理因素对子房未授粉南瓜胚形成的影响[J].植物生理学通讯,2009,45(10):977-980.SUN S R,ZHAI Q H,HU J B,et al.Effects of several physiological factors on embryo formation in unpollinated ovary culture of pumpkin[J].Plant Physiology Communication,2009,45(10):977-980.
[13]徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂[J].植物学通报,2003,20(2):227-237.XU X F,HUANG X L.TDZ:an efficacious plant growth regulator[J].Chinese Bulletin of Botany,2003,20(2):227-237.
[14]OUEDRAOGO J T,ST-PIERRE C A,COLLIN J,et al.Effect of amino acids grow regulators and genotype on androgenesis in barley[J].Plant Cell Tissue&Organ Culture,1998,53:59-66.
[15]刁卫平,陈劲枫,雷春,等.影响黄瓜未授粉子房培养胚发生因素的研究[J].南京农业大学学报,2008,31(1):137-140.DIAO W P,CHEN J F,LEI C,et al.Factors affecting embryo formation in unpollinated ovary culture of cucumber[J].Journal of Nanjing Agricultural University,2008,31(1):137-140.