寒葱提取物对肾阳虚大鼠SOD、MDA及睾丸组织的影响
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摘要
为探究寒葱对雄性"肾阳虚"大鼠氧化损伤的调节作用,对寒葱水提物和醇提物中的总酚和总黄酮进行定量测定;以8只大鼠为正常组,注射生理盐水,32只大鼠注射氢化可的松造模,造模2周成功后,32只肾阳虚大鼠均分为模型组、低剂量组、中剂量组和高剂量组,之后模型组和正常组灌胃生理盐水,其余各组灌胃相应剂量的寒葱水提物4周,同样方法进行寒葱醇提取物试验,灌胃结束后检测各组大鼠生殖器官系数和血清中的SOD活性、MDA及睾酮含量,显微镜观察睾丸细微结构。结果表明:注射氢化可的松的雄性大鼠出现明显的肾阳虚症状,生殖器官系数下降,血清中SOD活性和睾酮含量下降,MDA含量提高,睾丸曲细精管生精细胞排列紊乱、层次减少、管腔扩大,细胞处于变性阶段,但是寒葱提取物干预后大鼠阳虚症状减轻,生殖器官系数显著上升,血清的SOD活性和睾酮含量明显升高,MDA含量显著下降。高剂量组SOD的活性显著高于中、低剂量组(P<0.05),睾丸曲细精管生精上皮增生明显,细胞层次增多,排列紧密基本恢复到正常组大鼠状态。寒葱提取物可修复氧化损伤组织并改善肾阳虚症状。
To investigate the regulating effect of Allium Victorialis on oxidative injury in male rats with kidney-yang deficiency. The total phenols and flavonoids in the alcohol and water extracts of Allium Victorialis were quantitatively determined. Eight rats in normal group were injected physiological saline, thirty-two rats were built models by hydrocortisone injection. After 2 weeks, 32 rats with kidney Yang deficiency were divided into model group, low dose group, middle dose group and high dose group. The mice in model group and normal group were gavaged physiological saline, the mice in other groups were gavaged corresponding doses of Allium Victorialis water extract for 4 weeks. The method was same for Allium Victorialis alcohol extract test. The viscera coefficient, serum SOD and MDA activity, testosterone level and testicular fine structure were tested. The results showed that the rats appeared obvious symptoms of kidney Yang deficiency; viscera coefficient, serum SOD activity and testosterone content decreased, MDA concentration increased; testicular song fine exchange sperm cells arranged disorder, levels decrease, lumen enlarge, cell degeneration stage after hydrocortisone injection. However, Yang deficiency symptoms of rats alleviated, the viscera coefficient significantly increased, serum SOD activity and testosterone content significantly increased, MDA content significantly dropped after Allium victoriali extract intervention. SOD activity in the high-dose group was significantly higher than that in the medium-dose and low-dose groups(P<0.05). The hyperplasia of spermatogenic epithelium in the convoluted fine tubule of the testis was obvious, and the cells in the high-dose group were more layered and closely arranged, which basically returned to the normal state. It suggests that Allium Victorialis extract can restore oxidative damage and improve the symptoms of kidney Yang deficiency.
To investigate the regulating effect of Allium Victorialis on oxidative injury in male rats with kidney-yang deficiency. The total phenols and flavonoids in the alcohol and water extracts of Allium Victorialis were quantitatively determined. Eight rats in normal group were injected physiological saline, thirty-two rats were built models by hydrocortisone injection. After 2 weeks, 32 rats with kidney Yang deficiency were divided into model group, low dose group, middle dose group and high dose group. The mice in model group and normal group were gavaged physiological saline, the mice in other groups were gavaged corresponding doses of Allium Victorialis water extract for 4 weeks. The method was same for Allium Victorialis alcohol extract test. The viscera coefficient, serum SOD and MDA activity, testosterone level and testicular fine structure were tested. The results showed that the rats appeared obvious symptoms of kidney Yang deficiency; viscera coefficient, serum SOD activity and testosterone content decreased, MDA concentration increased; testicular song fine exchange sperm cells arranged disorder, levels decrease, lumen enlarge, cell degeneration stage after hydrocortisone injection. However, Yang deficiency symptoms of rats alleviated, the viscera coefficient significantly increased, serum SOD activity and testosterone content significantly increased, MDA content significantly dropped after Allium victoriali extract intervention. SOD activity in the high-dose group was significantly higher than that in the medium-dose and low-dose groups(P<0.05). The hyperplasia of spermatogenic epithelium in the convoluted fine tubule of the testis was obvious, and the cells in the high-dose group were more layered and closely arranged, which basically returned to the normal state. It suggests that Allium Victorialis extract can restore oxidative damage and improve the symptoms of kidney Yang deficiency.
引文
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[15] 刘静,赵海霞,吴秋月,等.基于ATR/Chk1/P53信号通路探讨三七总皂苷对自然衰老大鼠睾丸生殖细胞DNA损伤的保护作用[J].中草药,2017,48(05):935-940.
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[2] 朱淑云,周越,肖香,等.水飞蓟油的体外抗氧化活性及对氧化损伤小鼠的保护作用[J].食品科学,2018,39(05):234-238.
[3] 张丹,朱庆均.肾阳虚小鼠睾丸NO、NOS、MDA、SOD变化的实验研究[J].中国中医药科技,2009,16(2):84.
[4] 孟艳燕.氧化应激条件下自噬及PARP-1在血管平滑肌细胞中的作用及其关系的研究[D].沈阳:中国医科大学,2018.
[5] 武丽梅.韭菜籽有效成分的提取及其抗氧化活性分析[D].上海:华东理工大学,2012.
[6] 周蓉.韭菜改善性功能物质筛选及其功效鉴定的研究[D].杭州:浙江工商大学,2009.
[7] 王子建,李浩,李登科,等.何首乌水提物及其主要成分对人肝细胞L02中CYP1A2、CYP2C9和CYP2E1mRNA表达的影响[J].中草药,2017,48(23):4912-4920.
[8] 张凌云,叶迪,楼招欢.龙眼叶水提物抗实验性肾结石作用的研究[J].现代食品科技,2018,34(02):25-31
[9] 蔡文国,吴卫,代沙,等.不同种质鱼腥草总酚、黄酮含量及其抗氧化活性[J].食品科学,2013,34(07):42-46.
[10] 萨茹丽,木其尔,王翠芳,等.沙葱总黄酮提取工艺优化及其体外抗氧化、抗菌作用[J/OL].食品科学,2014,35(24):1-8.
[11] 邹忠杰,龚梦鹃,谢媛媛,等.氢化可的松诱导的肾阳虚大鼠尿液代谢组学研究[J].中国实验方剂学志,2012,18(08):133-136.
[12] 张子怡,陈宝军,张英杰,等.肾阳虚、肾阴虚证大鼠模型的建立与稳定性观察[J].福建中医药,2015,46(01):51-54.
[13] 练有文,王晖,倪少凯,等.BALB/c系裸小鼠脏器重量、脏器系数的测定[J].中国比较医学杂志,2006(05):285-287.
[14] 董延生,尹纪业,陈长,等.SD大鼠脏器重量及脏器系数正常参考值的确立与应用[J].军事医学,2012,36(05):351-353.
[15] 刘静,赵海霞,吴秋月,等.基于ATR/Chk1/P53信号通路探讨三七总皂苷对自然衰老大鼠睾丸生殖细胞DNA损伤的保护作用[J].中草药,2017,48(05):935-940.
[16] Archibong A E,Ramesh A,Niaz M S,et al.Effects of benzo(a)pyrene on intra-testicular function in F-344 rats[J].Int J Environ Res Public Health,2008,5(1):32-40.
[17] 崔颖.活性4维生素D_3对睾丸赖迪细胞氧化损伤的保护及机制研究[D].郑州:郑州大学,2018.
[18] 鲁亚星.寒葱活性成分分析及其咀嚼片制备工艺研究[D].延吉:延边大学,2018.
[19] Hu M C,Shiizaki K,Kuroo M,et al.Fibroblast growth factor 23 and klotho:physiology and pathophysiology of an endocrine network ofmineral metabolism[J].Annu Rev Physiol,2013,75:503-33.
[20] Fukumoto S.Phosphate metabolism and vitamin D[J].Bonekey Rep,2014(3):497.
[21] 嵇蓉,吴可.亚健康、氧化应激与抗氧化[J].实用预防医学,2007,14(6):1959.