红宝石球花报春腋芽再生体系的建立
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摘要
以红宝石球花报春(Primula denticulata)无菌苗腋芽为外植体,通过对丛生芽诱导、增殖、生根、炼苗和移栽等技术进行研究,筛选出各阶段的最佳培养基配方,建立了红宝石球花报春的再生体系。结果表明,适宜红宝石球花报春腋芽诱导不定芽的培养基为MS+1 mg·L~(-1) NAA+0.1 mg·L~(-1) 6-BA,诱导率达73.33%;适宜丛生芽增殖的培养基为MS+1 mg·L~(-1)NAA+0.5 mg·L~(-1) 6-BA,增殖率达85.19%;最佳生根培养基为1/2MS+0.2 mg·L~(-1) NAA,生根率达95.59%;最佳移栽基质为草炭:珍珠岩=3:1(v/v)的混合基质,移栽成活率可达96.67%。
The axillary buds of sterile seedlings of Primula denticulata were used as explants. By disinfecting the explants, inducing adventitious buds, multiplication of adventitious buds, rooting culture and transplantation, the best medium of each stage was selected to establish the regeneration system of P. denticulata. The optimal medium of P. denticulata for inducing adventitious shoots from explants was MS+1 mg·L~(-1) NAA+0.1 mg·L~(-1) 6-BA, and the induction rate was 73.33%. The optimal medium for inducing multiple shoots was MS+1 mg·L~(-1) NAA+0.5 mg·L~(-1) 6-BA, and the proliferation rate was 85.19%. The optimal medium for rooting was 1/2 MS+0.2 mg·L~(-1) NAA, and the rooting rate was 95.59%. Tissue cultured P. denticulata seedlings were transplanted into a mixture of peat and perlite, and the volume ratio was 3:1, with maximum survival rate 96.67%.
The axillary buds of sterile seedlings of Primula denticulata were used as explants. By disinfecting the explants, inducing adventitious buds, multiplication of adventitious buds, rooting culture and transplantation, the best medium of each stage was selected to establish the regeneration system of P. denticulata. The optimal medium of P. denticulata for inducing adventitious shoots from explants was MS+1 mg·L~(-1) NAA+0.1 mg·L~(-1) 6-BA, and the induction rate was 73.33%. The optimal medium for inducing multiple shoots was MS+1 mg·L~(-1) NAA+0.5 mg·L~(-1) 6-BA, and the proliferation rate was 85.19%. The optimal medium for rooting was 1/2 MS+0.2 mg·L~(-1) NAA, and the rooting rate was 95.59%. Tissue cultured P. denticulata seedlings were transplanted into a mixture of peat and perlite, and the volume ratio was 3:1, with maximum survival rate 96.67%.
引文
邓平平(2008).六种报春花属植物的组织培养与多倍体诱导研究.硕士论文.北京:北京林业大学.pp.1-2.
侯云屏,古志渊(2001).报春花的组织培养与快速繁殖.植物生理学通讯37,234-235.
贾茵(2010).小报春新品种选育研究.硕士论文.北京:北京林业大学.pp.1-2.
金晓霞,潘会堂,张启翔(2005).小报春的组织培养和植株再生.植物生理学通讯41,794.
李春玲,蒋钟仁,熊佑清(1992).早春野生花卉组织培养研究初报.园艺学报19,277-278.
李翠娟(2007).灯台组(Primula Sect.Proliferae)报春花栽培与杂交育种研究.硕士论文.北京:北京林业大学.pp.1-2.
李彦舫,张亚兰,杨松涛,杨文杰,庞劲松(1992).报春花的组织培养和植株再生.东北师大学报(自然科学版)(4),94-97.
刘芳,唐映红,袁有美,郭清泉,沈帆,陈建荣(2016).多肉植物劳尔的组织培养.植物学报51,251-256.
娄和林,李世承,韩阳,侯潇,李晶(1996).四季樱草叶肉原生质体植株再生.辽宁大学学报(自然科学版)23(4),88-91.
吕美萍,王元忠,黄衡宇(2016).地皮消愈伤组织诱导及植株高效再生体系的建立.植物学报51,89-97.
宋建英,叶建仁,陈剑勇(2007).鄂报春的组培技术研究.西南林学院学报27(2),53-56.
王莲辉,冯育才,韩克松,姜运力,潘得权,杨成华(2010).鄂报春组织培养与快速繁殖(简报).亚热带植物科学39(4),67-68.
解玮佳,李世峰,李涵,蒋亚莲,蔡艳飞,李树发(2010).滇北球花报春的组织培养(简报).亚热带植物科学39,81.
查帅兵(2006).安徽羽叶报春愈伤组织的诱导和培养.生物学杂志23(5),43-45,33.
张启翔,李翠娟,潘会堂,梁树乐,程堂仁,孙明(2007).滇北球花报春的组培快繁方法.中国专利:CN101015280.2010-03-03.
张倩(2012).牡丹组织培养中生根与移栽驯化研究进展.黑龙江农业科学(4),146-149.
张群,吕秀立,何小丽,朱义,崔心红(2016).海三棱藨草的组织培养与快繁体系.植物学报51,684-690.
张淑娟,刘与明,罗智凤,翁萍(2002).四季樱草的组织培养和快速繁殖.植物生理学通讯38,354.
赵妍,潘会堂,张启翔,王史琴,董玲玲(2010).岩生报春的组织培养与快速繁殖.植物生理学通讯46,1275-1276.
Furuya H,Hosoki T(2005).In vitro propagation of Primula sieboldii E.Morr.through root segment culture.Hortic Res(Japan)4,21-26.
Morozowska M,Weso?owska M(2004).In vitro clonal propagation of Primula veris L.and preliminary phytochemical analysis.Acta Biol Crac Ser Bot 46,169-175.
Yamamoto T,Magaya Y,Maruyama Y(1999).Mass propagation of Primula sieboldii E.Morr.through leaf segment culture.Bulletin of the Faculty of Horticulture Minamikyushu University(Series A.Natural Science)29,9-14.
侯云屏,古志渊(2001).报春花的组织培养与快速繁殖.植物生理学通讯37,234-235.
贾茵(2010).小报春新品种选育研究.硕士论文.北京:北京林业大学.pp.1-2.
金晓霞,潘会堂,张启翔(2005).小报春的组织培养和植株再生.植物生理学通讯41,794.
李春玲,蒋钟仁,熊佑清(1992).早春野生花卉组织培养研究初报.园艺学报19,277-278.
李翠娟(2007).灯台组(Primula Sect.Proliferae)报春花栽培与杂交育种研究.硕士论文.北京:北京林业大学.pp.1-2.
李彦舫,张亚兰,杨松涛,杨文杰,庞劲松(1992).报春花的组织培养和植株再生.东北师大学报(自然科学版)(4),94-97.
刘芳,唐映红,袁有美,郭清泉,沈帆,陈建荣(2016).多肉植物劳尔的组织培养.植物学报51,251-256.
娄和林,李世承,韩阳,侯潇,李晶(1996).四季樱草叶肉原生质体植株再生.辽宁大学学报(自然科学版)23(4),88-91.
吕美萍,王元忠,黄衡宇(2016).地皮消愈伤组织诱导及植株高效再生体系的建立.植物学报51,89-97.
宋建英,叶建仁,陈剑勇(2007).鄂报春的组培技术研究.西南林学院学报27(2),53-56.
王莲辉,冯育才,韩克松,姜运力,潘得权,杨成华(2010).鄂报春组织培养与快速繁殖(简报).亚热带植物科学39(4),67-68.
解玮佳,李世峰,李涵,蒋亚莲,蔡艳飞,李树发(2010).滇北球花报春的组织培养(简报).亚热带植物科学39,81.
查帅兵(2006).安徽羽叶报春愈伤组织的诱导和培养.生物学杂志23(5),43-45,33.
张启翔,李翠娟,潘会堂,梁树乐,程堂仁,孙明(2007).滇北球花报春的组培快繁方法.中国专利:CN101015280.2010-03-03.
张倩(2012).牡丹组织培养中生根与移栽驯化研究进展.黑龙江农业科学(4),146-149.
张群,吕秀立,何小丽,朱义,崔心红(2016).海三棱藨草的组织培养与快繁体系.植物学报51,684-690.
张淑娟,刘与明,罗智凤,翁萍(2002).四季樱草的组织培养和快速繁殖.植物生理学通讯38,354.
赵妍,潘会堂,张启翔,王史琴,董玲玲(2010).岩生报春的组织培养与快速繁殖.植物生理学通讯46,1275-1276.
Furuya H,Hosoki T(2005).In vitro propagation of Primula sieboldii E.Morr.through root segment culture.Hortic Res(Japan)4,21-26.
Morozowska M,Weso?owska M(2004).In vitro clonal propagation of Primula veris L.and preliminary phytochemical analysis.Acta Biol Crac Ser Bot 46,169-175.
Yamamoto T,Magaya Y,Maruyama Y(1999).Mass propagation of Primula sieboldii E.Morr.through leaf segment culture.Bulletin of the Faculty of Horticulture Minamikyushu University(Series A.Natural Science)29,9-14.