正交试验优选知母酒制工艺
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摘要
目的:优选酒制知母的炮制工艺,确定其工艺参数。方法:以知母皂苷BⅡ和芒果苷含量为考察指标,选取黄酒用量、焖制时间、炒制温度和炒制时间4个因素,应用L_9(3~4)正交设计表,采用方差分析对知母的酒制工艺优选。知母皂苷BⅡ色谱分离条件:色谱柱为CAPCELL PAK C_(18)(5μm,4.6 mm×250 mm),乙腈-水(25∶75)为流动相,流速1.0 mL·min~(-1);芒果苷的色谱分离条件:色谱柱为Waters Symmetry C_(18)(5μm,4.6 mm×150 mm),乙腈-0.2%乙酸溶液(15∶85)为流动相,流速1.0 mL·min~(-1),检测波长258 nm。结果:最佳炮制工艺参数:黄酒用量15 g,焖制时间90 min,炒制温度190℃,炒制时间25 min。结论:优选的炮制工艺稳定可行,为酒制知母提供实验基础。
Objective:To optimize the processing technology of wine roasted Anemarrhena for the optimum process. Methods:The contents of timosaponin B Ⅱ and mangiferin were selected as quality index. The wine volume,the moistening time,the processing time,and processing temperature were optimized as influencing factors by L_9(3~4)orthogonal design experiments. The chromatographic conditions for timosaponin B Ⅱ were as follows:a CAPCELL PAK C_(18) column(5 μm,4.6 mm×250 mm)was used at 30 ℃ with the mobile phase of acetonitrile-water(25∶75)at a flow rate of 1.0 mL·min~(-1). The chromatographic conditions for mangiferin were as follows:The separation was performed on a Waters Symmetry C_(18)(5 μm,4.6 mm×150 mm)column at 30 ℃ with the mobile phase of acetonitrile-0.2% acetic acid solution(15∶85). The elution was set at a flow rate of 1.0 mL·min~(-1) and the detection wavelength was set at 285 nm. Results:The best processing conditions were as follows:100 g Anemarrhena adding amount of 15 g yellow rice wine was mixed thoroughly and was placed for 90 min,then carbonized by stir-frying at 190 ℃ for 25 min.Conclusion:This optimized processing technology is stable and feasible,and can provide experiment basis for wine roasted Anemarrhena.
Objective:To optimize the processing technology of wine roasted Anemarrhena for the optimum process. Methods:The contents of timosaponin B Ⅱ and mangiferin were selected as quality index. The wine volume,the moistening time,the processing time,and processing temperature were optimized as influencing factors by L_9(3~4)orthogonal design experiments. The chromatographic conditions for timosaponin B Ⅱ were as follows:a CAPCELL PAK C_(18) column(5 μm,4.6 mm×250 mm)was used at 30 ℃ with the mobile phase of acetonitrile-water(25∶75)at a flow rate of 1.0 mL·min~(-1). The chromatographic conditions for mangiferin were as follows:The separation was performed on a Waters Symmetry C_(18)(5 μm,4.6 mm×150 mm)column at 30 ℃ with the mobile phase of acetonitrile-0.2% acetic acid solution(15∶85). The elution was set at a flow rate of 1.0 mL·min~(-1) and the detection wavelength was set at 285 nm. Results:The best processing conditions were as follows:100 g Anemarrhena adding amount of 15 g yellow rice wine was mixed thoroughly and was placed for 90 min,then carbonized by stir-frying at 190 ℃ for 25 min.Conclusion:This optimized processing technology is stable and feasible,and can provide experiment basis for wine roasted Anemarrhena.
引文
[1]中华人民共和国药典2015年版.一部[S].2015:212ChP 2015.VolⅠ[S].2015:212
[2]孙兴华,张宇伟,陈芳芳,等.知母中化学成分的研究[J].海峡药学,2015,27(8):40SUN XH,,ZHANG YW,CHEN FF,et al.Constituents from Anemarrhena asphodeloides Bge.[J].Strait Pharm J,2015,27(8):40
[3]李国龙,杨洁,段金廒,等.不同产地知母药材质量分析评价[J].中药材,2015,38(6):1148LI GL,YANG J,DUAN JA,et al.Quality analysis and evaluation of Anemarrhena asphodeloides rhizome from different habitats[J].J Chin Med Mater,2015,38(6):1148
[4]赵阳,康利平,余河水,等.利用UHPLC/Q-TOF MS技术进行7个甾体皂苷含量测定[J].中国药学,2013,22(3):226ZHAO Y,KANG LP,YU HS,et al.Simultaneous determination of steroidal saponins in Anemarrhena asphodeloides Bge.by ultra high-performance liquid chromatography/quadrupole time-offlight mass spectrometry[J].J Chin Pharm Sci,2013,22(3):226
[5]崔玲玲,季德,袁永亮,等.知母酒炙前后指纹图谱的比较及对α-葡萄糖苷酶抑制作用的比较研究[J].海峡药学,2016,28(4):43CUI LL,JI D,YUAN YL,et al.Comparison of fingerprints and inhibitory activities onα-glucosidase of crude and processed Rhizoma comparison on fingerprint and inhibitory activities onα-glucosidase of crude and processed Rhizoma Anemarrhena[J].Strait Pharm J,2016,28(4):43
[6]李习平,杨梓,石继连,等.不同炮制方法对知母中芒果苷含量的影响[J].时珍国医国药,2011,22(10):2502LI XP,YANG Z,SHI JL,et al.Effect of different processing methods on mangiferin content in Rhizoma Anemarrhenae[J].Lishizhen Med Mater Med Res,2011,22(10):2502
[7]冯倩茹,高妮.知母常用炮制工艺的比较研究[J].临床医学工程,2012,19(5):815FENG QR,GAO N.A comparative study on processing technology of Anemarrhenae Rhizoma[J].Clin Med Eng,2012,19(5):815
[8]马河,刘园华,张金杰.知母水溶性小分子提取物对胰岛素抵抗Hep G2细胞体外糖代谢的影响[J].食品与药品,2013,15(1):26MA H,LIU YH,ZHANG JJ.Effect of water-soluble small molecule extract in Anemarrhenae Rhizoma on glucose metabolism in insulinresistant HepG2 cells[J].Food Drug,2013,15(1):26
[9]路明珠,张治强,伊佳,等.知母皂苷B-Ⅱ抗抑郁作用及其机制研究[J].药学实践杂志,2010,28(4):283LU MZ,ZHANG ZQ,YI J,et al.Study on the effect and mechanisms of timosaponin B-Ⅱon antidepressant[J].J Pharm Pract,2010,28(4):283
[10]张志斐,肖蓉,袁志芳,等.河北道地药材知母HPLC-ELSD指纹图谱研究[J].药物分析杂志,2006,26(11):1569ZHANG ZF,XIAO R,YUAN ZF,et al.Studies on HPLC-ELSD fingerprints of crude Rhizoma Anemarrhenae from Hebei Province[J].Chin J Pharm Anal,2006,26(11):1569
[11]赵璐璐,刘菲菲,彭缨,等.高效液相色谱法考察不同炮制方法对知母中5种主要化学成分的影响[J].色谱,2012,12(30):1271ZHAO LL,LIU FF,PENG Y,et al.Effects of different processing methods on five main chemical constituents of Anemarrhena asphodeloidesBge.studiedbyhighperformanceliquid chromatography[J].Chin J Chromatogr,2012,12(30):1271
[12]魏静娜,刘正辉,赵琳琳,等.知母的盐制工艺优化[J].辽宁中医杂志,2015,42(6):1294WEI JN,LIU ZH,ZHAO LL,et al.Optimization of salted processing for Anemarrhena[J].Liaoning J Tradit Chin Med,2015,42(6):1294
[13]宋泽璧,吴莹,高慧.HPLC法测定盐炙前后知母中新芒果苷、芒果苷和异芒果苷[J].现代药物与临床,2015,30(2):145SONG ZB,WU Y,GAO H.Determination of neomangiferin,mangiferin,and isomangiferin in Anemarrhenae Rhizoma before and after stir-frying with salt-water by HPLC[J].Drugs Clin,2015,30(2):145
[2]孙兴华,张宇伟,陈芳芳,等.知母中化学成分的研究[J].海峡药学,2015,27(8):40SUN XH,,ZHANG YW,CHEN FF,et al.Constituents from Anemarrhena asphodeloides Bge.[J].Strait Pharm J,2015,27(8):40
[3]李国龙,杨洁,段金廒,等.不同产地知母药材质量分析评价[J].中药材,2015,38(6):1148LI GL,YANG J,DUAN JA,et al.Quality analysis and evaluation of Anemarrhena asphodeloides rhizome from different habitats[J].J Chin Med Mater,2015,38(6):1148
[4]赵阳,康利平,余河水,等.利用UHPLC/Q-TOF MS技术进行7个甾体皂苷含量测定[J].中国药学,2013,22(3):226ZHAO Y,KANG LP,YU HS,et al.Simultaneous determination of steroidal saponins in Anemarrhena asphodeloides Bge.by ultra high-performance liquid chromatography/quadrupole time-offlight mass spectrometry[J].J Chin Pharm Sci,2013,22(3):226
[5]崔玲玲,季德,袁永亮,等.知母酒炙前后指纹图谱的比较及对α-葡萄糖苷酶抑制作用的比较研究[J].海峡药学,2016,28(4):43CUI LL,JI D,YUAN YL,et al.Comparison of fingerprints and inhibitory activities onα-glucosidase of crude and processed Rhizoma comparison on fingerprint and inhibitory activities onα-glucosidase of crude and processed Rhizoma Anemarrhena[J].Strait Pharm J,2016,28(4):43
[6]李习平,杨梓,石继连,等.不同炮制方法对知母中芒果苷含量的影响[J].时珍国医国药,2011,22(10):2502LI XP,YANG Z,SHI JL,et al.Effect of different processing methods on mangiferin content in Rhizoma Anemarrhenae[J].Lishizhen Med Mater Med Res,2011,22(10):2502
[7]冯倩茹,高妮.知母常用炮制工艺的比较研究[J].临床医学工程,2012,19(5):815FENG QR,GAO N.A comparative study on processing technology of Anemarrhenae Rhizoma[J].Clin Med Eng,2012,19(5):815
[8]马河,刘园华,张金杰.知母水溶性小分子提取物对胰岛素抵抗Hep G2细胞体外糖代谢的影响[J].食品与药品,2013,15(1):26MA H,LIU YH,ZHANG JJ.Effect of water-soluble small molecule extract in Anemarrhenae Rhizoma on glucose metabolism in insulinresistant HepG2 cells[J].Food Drug,2013,15(1):26
[9]路明珠,张治强,伊佳,等.知母皂苷B-Ⅱ抗抑郁作用及其机制研究[J].药学实践杂志,2010,28(4):283LU MZ,ZHANG ZQ,YI J,et al.Study on the effect and mechanisms of timosaponin B-Ⅱon antidepressant[J].J Pharm Pract,2010,28(4):283
[10]张志斐,肖蓉,袁志芳,等.河北道地药材知母HPLC-ELSD指纹图谱研究[J].药物分析杂志,2006,26(11):1569ZHANG ZF,XIAO R,YUAN ZF,et al.Studies on HPLC-ELSD fingerprints of crude Rhizoma Anemarrhenae from Hebei Province[J].Chin J Pharm Anal,2006,26(11):1569
[11]赵璐璐,刘菲菲,彭缨,等.高效液相色谱法考察不同炮制方法对知母中5种主要化学成分的影响[J].色谱,2012,12(30):1271ZHAO LL,LIU FF,PENG Y,et al.Effects of different processing methods on five main chemical constituents of Anemarrhena asphodeloidesBge.studiedbyhighperformanceliquid chromatography[J].Chin J Chromatogr,2012,12(30):1271
[12]魏静娜,刘正辉,赵琳琳,等.知母的盐制工艺优化[J].辽宁中医杂志,2015,42(6):1294WEI JN,LIU ZH,ZHAO LL,et al.Optimization of salted processing for Anemarrhena[J].Liaoning J Tradit Chin Med,2015,42(6):1294
[13]宋泽璧,吴莹,高慧.HPLC法测定盐炙前后知母中新芒果苷、芒果苷和异芒果苷[J].现代药物与临床,2015,30(2):145SONG ZB,WU Y,GAO H.Determination of neomangiferin,mangiferin,and isomangiferin in Anemarrhenae Rhizoma before and after stir-frying with salt-water by HPLC[J].Drugs Clin,2015,30(2):145