摘要
为吉祥草叶斑病的综合防治提供科学依据,测定了吉祥草叶斑病菌细极链格孢(Alternaria tenuissima)的生物学特性及筛选适合的防治药剂。结果表明,PDA为最适培养基,病原菌生长适温为28℃,致死温度为45℃;在p H值为4~10的范围内均能生长,最适p H值为5;碳源和氮源以葡萄糖和硝酸铵较适宜;光照条件以12/12 h(光/暗)为佳。在供试的5种药剂中,10%苯醚甲环唑对病原菌抑菌效果最好,在推荐使用浓度1.00 mg/L下,相对抑菌率达到100%,而45%的石硫合剂抑菌效果最差,在推荐使用浓度6.67 mg/L下,其抑菌率仅为49.7%。
In order to provide scientific basis for the disease control,the biological characteristics and suitable pesticides for Reineckea carnea leaf spot pathogen Alternaria tenuissima were investigated. The results indicated that the pathogen mycelium were grown well in PDA under28 ℃,and the lethal temperature was 45 ℃. Although the mycelium could grow between p H 4. 0 to 10. 0,p H = 5. 0 was appropriate. Dextrose and NH4NO3 were the suitable carbon and nitrogen resource respectively. The light-dark alternation( 12 /12h) was benefit for mycelial growth. Among the 5 tested pesticides,the most effective agent was 10 % difenoconazole,the relative inhibition rate reached 100 % under the recommended concentration 1. 00 mg / L. Lime sulfur was the least effective agent,and the relative inhibition rate was only 49. 7 % under the recommended concentration 6. 67 mg / L.
引文
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