一株野生柳生田头菇菌株分离鉴定及驯化栽培
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摘要
以采自柳树腐木上的野生食用菌菌株KEFI-010为试材,通过形态学特征和ITS序列分析进行鉴定,并开展了驯化栽培试验研究,以期为柳生田头菇优良菌种选育及栽培技术研究提供参考依据。结果表明:该菌株为柳生田头菇(Agrocybe salicacicola),其菌丝生长速度快、洁白、浓密、整齐,栽培周期较短,出菇整齐、健壮,菌盖光滑,大小均匀,菌肉厚,菌柄粗,农艺性状较好,生物学效率为51.49%。子实体营养成分分析发现,该菌株蛋白质含量高,粗纤维、矿物质和氨基酸非常丰富,还含有人体必需的微量元素硒,具有较高的保健价值,是一种开发潜力巨大的食药用菌。
Wild edible fungi strain KEFI-010 collected from Salix matsudana was identified by morphological characteristics and ITS sequence analysis,domestication and cultivation experiments were also carried out on it,in order to provide a theoretical basis for the breeding of quality strains Agrocybe salicacicola of and the research of cultivation techniques.The results showed that the strain was Agrocybe salicacicola,and its mycelium grew fast,and was white,dense and neat.The cultivation cycle was short.The fruiting bodies grew orderly and strong,the cap was smooth,uniform in size,rich meat and sturdy stalk.The strain had good agronomic characters and high biological efficiency of 51.49%.The nutrient analysis of fruiting body from the strain showed that it had high protein content,rich in crude fiber,minerals and amino acids,and also contained trace element selenium,which was essential for human body.In conclusion, Agrocybe salicacicola had high health value with great potential for development.
Wild edible fungi strain KEFI-010 collected from Salix matsudana was identified by morphological characteristics and ITS sequence analysis,domestication and cultivation experiments were also carried out on it,in order to provide a theoretical basis for the breeding of quality strains Agrocybe salicacicola of and the research of cultivation techniques.The results showed that the strain was Agrocybe salicacicola,and its mycelium grew fast,and was white,dense and neat.The cultivation cycle was short.The fruiting bodies grew orderly and strong,the cap was smooth,uniform in size,rich meat and sturdy stalk.The strain had good agronomic characters and high biological efficiency of 51.49%.The nutrient analysis of fruiting body from the strain showed that it had high protein content,rich in crude fiber,minerals and amino acids,and also contained trace element selenium,which was essential for human body.In conclusion, Agrocybe salicacicola had high health value with great potential for development.
引文
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[15] 况丹.七种食用菌营养成分分析比较[J].食用菌,2011(4):57-59.
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[18] 李玲飞,蔡松伟,吴根福.富硒食用菌及其保健功效[J].食药用菌,2011,19(6):38-43.
[19] 陈析羽,张浩,汤虎,等.富硒食品的研究进展与展望[J].中国食物与营养,2018,24(6):11-14.
[2] 藏穆,杨祝良,刘学系.一种滇产美味新食用菌杨柳田头菇[J].中国食用菌,1991,9(6):18.
[3] 李玉,李泰辉,杨祝良,等.中国大型菌物资源图鉴[M].郑州:中原农民出版,2015.
[4] 戴玉成,周丽伟,杨祝良,等.中国食用菌名录[J].菌物学报,2010,29(1):15-21.
[5] 汪欣,刘平.杨柳田头菇引种驯化试验研究[J].中国食用菌,2004,23(2):16-17.
[6] 周会明.杨柳田头菇生活史及分类地位研究[D].昆明:昆明理工大学,2011.
[7] 周会明,赵永昌,陈卫民,等.柳田头菇交配型因子与菌丝生长速度关系[J].云南植物研究,2010,32(4):315-322.
[8] 何莹莹,陈卫民,赵永昌,等.云南田头菇属两个物种遗传多样性的AFLP分析[J].北方园艺,2012(21):85-88.
[9] 刘学系.杨柳田头菇与柱状田头菇子实体不同发育期形态比较[J].中国食用菌,1991,9(6):50-51.
[10] 王贺祥,刘庆洪.食用菌栽培学[M].北京:中国农业大学出版社,2014.
[11] 史琦云,邵威平.八种食用菌营养成分的测定与分析[J].甘肃农业大学学报,2003,38(3):336-339.
[12] 陈小保,谭育林,褚武英.茶树菇-Ws子实体营养成分分析研究[J].安徽农业科学,2013,41(29):11851,11880.
[13] 姜萍萍,韩烨,顾赛红,等.五种食用菌氨基酸含量的测定及营养评价[J].氨基酸和生物资源,2009,31(2):67-71.
[14] 王恒生,陈克龙,李怡,等.茶树菇的经济价值及开发用用[J].中国园艺文摘,2015(2):41-43.
[15] 况丹.七种食用菌营养成分分析比较[J].食用菌,2011(4):57-59.
[16] MARGARET P R.The importance of selenium to human health (review)[J].The Lancet,2000,356:233-241.
[17] 凌宏通,宋斌,林群英,等.富硒食用菌的研究进展[J].微生物学杂志,2008,28(4):78-81.
[18] 李玲飞,蔡松伟,吴根福.富硒食用菌及其保健功效[J].食药用菌,2011,19(6):38-43.
[19] 陈析羽,张浩,汤虎,等.富硒食品的研究进展与展望[J].中国食物与营养,2018,24(6):11-14.