基于灰色关联度与正交偏最小二乘法分析的苍术挥发油燥性谱效关系研究
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摘要
目的研究苍术挥发油的气相色谱-质谱(GC-MS)联用指纹图谱与苍术燥性效应的谱效关系,初步明确苍术主要燥性成分。方法采用GC-MS法对不同批次苍术(S1～S9)挥发油进行分析,以大鼠日均饮水量、全血黏度、肾脏中水通道蛋白2(AQP2)量为苍术燥性效应药效指标,采用灰色关联度分析法和正交偏最小二乘法相结合分析其谱效关系。结果苍术发挥燥性效应是多种成分共同作用的结果,对燥性作用药效贡献较大的3个峰分别为31、28、33号峰,经过谱库检索分析鉴定出3个峰对应的成分分别为β-桉叶醇、马兜铃烯、异愈创木醇。结论初步明确苍术主要燥性成分有β-桉叶醇、马兜铃烯、异愈创木醇,为苍术燥性物质基础研究提供参考。
Objective To study the spectrum-effect relationship between GC-MS fingerprint of the volatile oil of Atractylodis Rhizoma and the dryness effect of Atractylodis Rhizoma, and clarify the main dryness components of Atractylodis Rhizoma. Methods The volatile oil of different batches of Atractylodis Rhizoma(S1—S9) were analyzed by GC-MS. Using the daily drinking amount of water, the viscosity of whole blood, and the content of aquaporin 2(AQP2) in the kidney of rats as the indexes of dryness effect ofAtractylodes Rhizome, the spectrum-effect relationship was analyzed by the combination of grey relational grade and orthogonal partial least square method. Results The dryness effect of Atractylodis Rhizoma was the combined action of multiple components, and the peaks of the major contribution to dryness effect were 31, 28, and 33, respectively. The corresponding components of the three peaks were β-eudesmol,(-)-aristolene, and bulnesol, which were identified by the spectral library retrieval analysis. Conclusion The main dryness effect components of Atractylodis Rhizoma are β-eudesmol,(-)-aristolene, and bulnesol, which provide a reference for investigating the material basis of dryness effect of Atractylodis Rhizoma.
Objective To study the spectrum-effect relationship between GC-MS fingerprint of the volatile oil of Atractylodis Rhizoma and the dryness effect of Atractylodis Rhizoma, and clarify the main dryness components of Atractylodis Rhizoma. Methods The volatile oil of different batches of Atractylodis Rhizoma(S1—S9) were analyzed by GC-MS. Using the daily drinking amount of water, the viscosity of whole blood, and the content of aquaporin 2(AQP2) in the kidney of rats as the indexes of dryness effect ofAtractylodes Rhizome, the spectrum-effect relationship was analyzed by the combination of grey relational grade and orthogonal partial least square method. Results The dryness effect of Atractylodis Rhizoma was the combined action of multiple components, and the peaks of the major contribution to dryness effect were 31, 28, and 33, respectively. The corresponding components of the three peaks were β-eudesmol,(-)-aristolene, and bulnesol, which were identified by the spectral library retrieval analysis. Conclusion The main dryness effect components of Atractylodis Rhizoma are β-eudesmol,(-)-aristolene, and bulnesol, which provide a reference for investigating the material basis of dryness effect of Atractylodis Rhizoma.
引文
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[2]王瑾,王强,李鹏,等.中药燥性理论溯源及研究思路探讨[J].中国中药杂志,2014,39(2):346-348.
[3]瞿领航,涂济源,曹国胜,等.基于燥胜则干和燥性伤阴理论的苍术燥性效应研究[J].中国中药杂志,2018,43(13):2705-2712.
[4]白关亚,何盼,李媛媛,等.青翘不同极性部位抗炎作用的谱效关系分析[J].中国实验方剂学杂志,2017,23(11):1-6.
[5]林梦雅,张玉萍,李雅,等.基于灰色关联度分析的丹参提取物抗炎作用谱效关系研究[J].中草药,2017,48(16):3447-3452.
[6]卿勇军,刘文,杜平,等.基于灰色关联度分析戊己丸对胃蛋白酶抑制作用的谱效关系[J].中草药,2017,48(16):3390-3395.
[7]胡露,张锦,蔺良才,等.基于谱效关系的头花蓼抑菌作用物质基础研究[J].中药材,2016,39(9):2037-2040.
[8]李力,潘倩雯,刘宏.灰色关联度分析法在中药谱效学研究中的应用[J].中国药房,2018,29(11):1581-1584.
[9]贾富霞,罗容,王秀娟.谱效关系研究方法在中药研究中的应用进展[J].北京中医药,2015,34(12):996-1000.
[10]胡平,村上政隆,张前德,等.养阴清热活血中药对大鼠离体灌流颌下腺唾液分泌的作用[J].微循环学杂志,2010,20(4):22-25.
[11]许晨曦,刘玉强,张丝雨,等.生、麸炒苍术对大鼠AQP1、AQP5及血液流变学的影响[J].中药材,2015,38(10):2056-2059.
[12]刘艳菊,陈雯雯,曾敏,等.苍术炮制前后水提物药效学研究[J].中国中药杂志,2012,37(15):2276-2279.
[13]陈雯雯.苍术麸炒前后氯仿和挥发油部位药效学及化学成分对比研究[D].武汉:湖北中医药大学,2013.
[14]Shida Y,Matsuoka H,Chiga M,et al.Characterization of AQP-2 gene mutation(R254Q)in a family with dominant nephrogenic DI[J].Pediatr Int,2013,55(1):105-107.
[15]赵青红.麸炒对苍术挥发油的影响及其与道地性的关系[D].北京:中国中医科学院,2009.
[16]周洁,郭兰萍,黄璐琦,等.基于道地药材标准的炮制苍术挥发油变化规律研究[J].中国药学杂志,2009,44(8):567-570.
[17]龚先玲,典灵辉,张立坚,等.水菖蒲根状茎与根挥发油化学成分研究[J].中国药房,2007,18(3):176-178.
[18]吴惠勤.广防己挥发油的GC-MS指纹图谱研究[A]//2004年全国有机质谱学术交流会论文集[C].北京:中国分析测试学会,2004.
[19]邓芹英,黄维德,李考铮.缬草根化学成分的研究[J].分析测试学报,1995,14(2):16-22.
[20]王世永.吴茱萸挥发油的提取分离、鉴定及抗氧化和抗菌活性研究[D].武汉:华中农业大学,2008.
[21]耿晓萍,石晋丽,刘勇,等.甘松地上和地下部位挥发油化学成分比较研究[J].北京中医药大学学报,2011,34(1):56-59.