谷胱甘肽S-转移酶P1 Ile(105)Val基因多态性与河南回族慢性阻塞性肺疾病易感性的关系
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摘要
目的探讨谷胱甘肽S转移酶P1(GSTP1)Ile(105)Val基因多态性与河南回族的慢性阻塞性肺疾病(COPD)易感性的关系。方法将2013年1月至2017年12月该院收治的住院及门诊患者100例分为对照组(排除COPD、支气管哮喘、慢性支气管炎及其他慢性呼吸系统疾病者)和COPD组,均为河南回族患者,每组50例。采用限制性片段长度多态性聚合酶链反应检测两组患者GSTP1基因型。结果两组患者GSTP1基因型频率比较,差异无统计学意义(P>0.05)。COPD组患者GSTP1第5外显子105位G等位基因频率[26.0%(26/100)]明显高于对照组[14.0%(14/100)],差异有统计学意义(P<0.05)。结论GSTP1 5号外显子G等位基因为河南回族COPD的独立危险因素。
Objective To discuss the relationship between the gene polymorphism of GSTP1 Ile(105)Val and the susceptility of Chronic Obstructive Pulmonary Disease(COPD).Methods 100 inpatients and outpatients admitted from January 2013 to December 2017 were selected in the study and divided into control group(patients without COPD,bronchial asthma,chronic bronchitis and other chronic respiratory diseases),and COPD group,and all patients were Hui nationality population in Henan,50 cases in each group.Restriction fragment length polymorphism polymerase chain reaction was used to detect GSTP1 genotype in two groups.Results There was no statistical significance in GSTP1 genotype frequency between two groups(P>0.05).The frequency of G allele at 105 position of exon 5 of GSTP1 in COPE group(26.0%)was higher than that in the control group(14.0%),and the difference was statistically significant(P<0.05).Conclusion The G allele of exon 5 of GSTP1 is an independent risk factor for Henan Hui nationality COPD patients.
Objective To discuss the relationship between the gene polymorphism of GSTP1 Ile(105)Val and the susceptility of Chronic Obstructive Pulmonary Disease(COPD).Methods 100 inpatients and outpatients admitted from January 2013 to December 2017 were selected in the study and divided into control group(patients without COPD,bronchial asthma,chronic bronchitis and other chronic respiratory diseases),and COPD group,and all patients were Hui nationality population in Henan,50 cases in each group.Restriction fragment length polymorphism polymerase chain reaction was used to detect GSTP1 genotype in two groups.Results There was no statistical significance in GSTP1 genotype frequency between two groups(P>0.05).The frequency of G allele at 105 position of exon 5 of GSTP1 in COPE group(26.0%)was higher than that in the control group(14.0%),and the difference was statistically significant(P<0.05).Conclusion The G allele of exon 5 of GSTP1 is an independent risk factor for Henan Hui nationality COPD patients.
引文
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[16]凌敏,安海燕,荣艳,等.谷胱甘肽S-转移酶P1基因多态性与哈萨克族慢性阻塞性肺疾病患者易感性的关系[J].中华结核与呼吸杂志,2013,36(6):459-460.
[17]LAKHDAR R,DENDEN S,MOUHAMED MH,et al.Correlation of EPHX1,GSTP1,GSTM1,and GSTT1genetic polymorphisms with antioxidative stress markers in chronic obstructive pulmonary disease[J].Exp Lung Res,2011,37(4):195-204.
[18]ZUNTAR I,PETLEVSKI R,DODIG S,et al.GSTP1,GSTM1and GSTT1genetic polymorphisms and total serum GSTconcentration in stable male COPD[J].Acta Pharm,2014,64(1):117-129.
[2]PILLAI SG,GE D,ZHU G,et al.A genome-wide association study in chronic obstructive pulmonary disease(COPD):identification of two major susceptibility loci[J].PLoS Genet,2009,5(3):e1000421.
[3]TIAN L,ZHANG J,XIAO S,et al.Impact of polymorphisms of the GGCX gene on maintenance warfarin dose in Chinese populations:systematic review and meta-analysis[J].Meta Gene,2015,5:43-54.
[4]YANG L,LI X,TONG X,et al.Association between glutathione S-transferase P1Ile(105)Val gene polymorphismand chronic obstructive pulmonary disease:a meta-analysis based on seventeencase-control studies[J].Meta Gene,2015,6:59-64.
[5]SMOLONSKA J,WIJMENGA C,POSTMA DS,et al.Meta-analyses on suspected chronic obstructive pulmonary disease genes:a summary of 20years′research[J].Am J Respir Crit Care Med,2009,180(7):618-631.
[6]YANG P,HO JW,YANG YH,et al.Gene-gene interaction filtering with ensemble of filters[J].BMC Bioinformatics,2011,12Suppl 1:S10.
[7]YAN F,CHEN C,JING J,et al.Association between polymorphism of glutathione S-transferase P1and chronic obstructive pulmonary disease:a meta-analysis[J].Respir Med,2010,104(4):473-480.
[8]中华医学会呼吸病学分会慢性阻塞性肺疾病(下转第1173页)学组.慢性阻塞性肺疾病诊治指南(2007年修订版)[J].中华结核和呼吸杂志,2007,30(1):8-17.
[9]LAKHDAR R,DENDEN S,KNANI J,et al.Combined analysis of EPHX1,GSTP1,GSTM1and GSTT1gene polymorphisms in relation to chronic obstructive pulmonary disease risk and lung function impairment[J].Dis Markers,2011,30(5):253-263.
[10]CURJURIC I,IMBODEN M,NADIF R,et al.Different genes interact with particulate matter and tobacco smoke exposure in affecting lung function decline in the general population[J].PLoSOne,2012,7(7):e40175.
[11]MORDUKHOVICH I,LEPEULE J,COULL BA,et al.The effect of oxidative stress polymorphisms on the association between long-term black carbon exposure and lung function among elderly men[J].Thorax,2015,70(2):133-137.
[12]LI X,LIU Z,DENG Y,et al.Modification of the association between maternal smoke exposure and congenital heart defects by polymorphisms in glutathione S-transferase genes[J].Sci Rep,2015,5:14915.
[13]STANKOVIC M,NIKOLIC A,TOMOVIC A,et al.Association of Functional Variants of Phase I and II Genes with Chronic Obstructive Pulmonary Disease in a Serbian Population[J].J Med Biochem,2015,34(2):207-214.
[14]LAKHDAR R,DENDEN S,KNANI J,et al.Relationship between glutathione S-transferase P1polymorphisms and chronicobstructive pulmonary disease in a Tunisian population[J].Genet Mol Res,2010,9(2):897-907.
[15]KORYTINA GF,AKHMADISHINA LZ,TSELOUSOVA OS,et al.Polymorphism of the genes for antioxidant defense enzymes and their association with the development of chronic obstructive pulmonary disease in the population of Bashkortostan[J].Genetika,2009,45(7):967-976.
[16]凌敏,安海燕,荣艳,等.谷胱甘肽S-转移酶P1基因多态性与哈萨克族慢性阻塞性肺疾病患者易感性的关系[J].中华结核与呼吸杂志,2013,36(6):459-460.
[17]LAKHDAR R,DENDEN S,MOUHAMED MH,et al.Correlation of EPHX1,GSTP1,GSTM1,and GSTT1genetic polymorphisms with antioxidative stress markers in chronic obstructive pulmonary disease[J].Exp Lung Res,2011,37(4):195-204.
[18]ZUNTAR I,PETLEVSKI R,DODIG S,et al.GSTP1,GSTM1and GSTT1genetic polymorphisms and total serum GSTconcentration in stable male COPD[J].Acta Pharm,2014,64(1):117-129.