三七总皂苷对肝癌HepG2细胞增殖、侵袭、凋亡及酶活性的影响
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摘要
目的:探讨三七总皂苷(PNS)对肝癌HepG2细胞增殖、迁移、酶活性及凋亡的影响。方法:应用不同浓度的PNS分别作用于HepG2细胞24、48、72h,采用MTT法测定细胞增殖率,Transwell实验检测肝癌细胞侵袭能力,流式细胞仪检测细胞凋亡水平,全自动生化分析仪检测肝癌细胞酶活性的改变。结果:PNS能抑制肝癌HepG2细胞的增殖和迁移,降低肝癌细胞酶活性,促进肝癌细胞的凋亡。
Objective:To investigate the effects of panax notoginseng saponins(PNS) on the cell proliferation,invasion,apoptosis and enzyme activity of liver cancercell lines HepG2. Methods:HepG2 cells were treated with different density of PNS for 24,48,72 h,Cell proliferation rate was detected by MTT assay; cell invasion ability was detected by Transwell method; the level of apoptosis was detected by flow cytometry,and the change of enzyme activity of liver cancer cells was detected by automatic biochemical analyzer. Conclusion:PNS could inhibit the proliferation and invasion of HepG2 cells,reduce the enzyme activity of hepatoma cells,and promote the apoptosis of hepatoma cells.
Objective:To investigate the effects of panax notoginseng saponins(PNS) on the cell proliferation,invasion,apoptosis and enzyme activity of liver cancercell lines HepG2. Methods:HepG2 cells were treated with different density of PNS for 24,48,72 h,Cell proliferation rate was detected by MTT assay; cell invasion ability was detected by Transwell method; the level of apoptosis was detected by flow cytometry,and the change of enzyme activity of liver cancer cells was detected by automatic biochemical analyzer. Conclusion:PNS could inhibit the proliferation and invasion of HepG2 cells,reduce the enzyme activity of hepatoma cells,and promote the apoptosis of hepatoma cells.
引文
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[9]陈培丰,刘鲁明,陈震,等.参三七醇提取液抑制肿瘤肝侵袭作用机理的实验研究[J].中国中医药科技,2007,14(2):96-97.
[10]程桂丹,陆枫林.肝癌中基质金属蛋白酶及其抑制因子的研究进展[J].东南大学学报(医学版),2010,29(2):215-220.
[2]Fan JS,Liu DN,Huang G,et al. Panax notoginseng saponins attenuate atherosclerosis via reciprocal regulation of lipid metabolism and inflammation by inducing liver X receptor alpha expression[J]. J Ethnopharmacol,2012,142(3):732-738.
[3]Wang P,Cui J,Du X,et al. Panax notoginseng saponins(PNS)inhibits breast cancer metastasis[J]. J Ethnopharmacol,2014,154(3):663-671.
[4]He NW,Zhao Y,Guo L,et al. Antioxidant,antiproliferative,and pro-apoptotic activities of a saponin extract derived from the roots of Panax notoginseng(Burk.)F.H. Chen[J].J Med Food,2012,15(4):350-359.
[5]Tao K,Fang M,Alroy J,et al. Imagable 4T1 model for the study of late stage breast cancer[J]. BMC Cancer,2008,8:228.
[6]Shibata MA,Ambati J,Shibata E,et al. Mammary cancer gene therapy targeting lymphangiogenesis:VEGF-C siRNA and soluble VEGF receptor-2,a splicing variant[J]. Med Mol Morphol,2012,45(4):179-184.
[7]王军,刘德慧,郑江涛,等.原发性肝癌临床治疗研究进展[J].中国老年学杂志,2010,30(3):429-431.
[8]李青,孙燕.肝癌靶向治疗的临床研究进展[J].中国新药杂志,2010,19(17):1547-1550.
[9]陈培丰,刘鲁明,陈震,等.参三七醇提取液抑制肿瘤肝侵袭作用机理的实验研究[J].中国中医药科技,2007,14(2):96-97.
[10]程桂丹,陆枫林.肝癌中基质金属蛋白酶及其抑制因子的研究进展[J].东南大学学报(医学版),2010,29(2):215-220.