枸杞多糖对卵形鲳鲹生长性能、抗氧化能力及血清免疫、生化指标的影响
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摘要
本试验旨在研究枸杞多糖对卵形鲳鲹(Trachinotus ovatus)生长性能、抗氧化能力及血清免疫、生化指标的影响。选取平均体质量为(7.45±0.06) g的卵形鲳鲹幼鱼360尾,随机分为6组,每组3个重复,每个重复20尾,分别饲喂枸杞多糖含量为0(对照)、0.05%、0.10%、0.20%、0.40%和0.80%的6种饲料。试验期8周。结果显示:1)各组末质量、增重率和特定生长率差异不显著(P>0.05)。2) 0.10%和0.20%组肝脏超氧化物歧化酶活性显著高于对照组(P<0.05),丙二醛含量显著低于对照组(P<0.05)。0.10%、0.20%、0.40%和0.80%组肝脏过氧化氢酶活性显著高于对照组(P<0.05)。0.10%组肝脏谷胱甘肽过氧化物酶活性和总抗氧化能力显著高于对照组(P<0.05)。3) 0.10%组血清补体3和补体4含量均显著高于对照组(P<0.05)。0.10%和0.20%组血清溶菌酶活性显著高于对照组(P<0.05)。4) 0.10%和0.20%组血清葡萄糖含量显著低于0.05%和对照组(P<0.05)。0.10%、0.20%和0.40%组血清胆固醇含量显著低于0.05%和对照组(P<0.05)。0.10%组血清甘油三酯含量显著低于0.05%和对照组(P<0.05)。0.10%和0.20%组血清高密度脂蛋白含量显著高于对照组(P<0.05),低密度脂蛋白含量显著低于对照组(P<0.05)。综上所述,枸杞多糖可以显著提高卵形鲳鲹的抗氧化能力和免疫力,而促生长的作用不显著。卵形鲳鲹幼鱼饲料中枸杞多糖的适宜添加量为0.10%。
To study the effects of Lycium barbarum polysaccharide(LBP) on growth performance,antioxidant capacity,serum immune and biochemical indexes of juvenile golden pompano(Trachinotus ovatus),six diets were prepared with the addition of 0(control),0.05%,0.10%,0.20%,0.40% and 0.80% LBP,respectively.A total of 360 fish with an average body weight of(7.45±0.06) g were assigned randomly into six groups with three replicates per group and 20 fish per replicate.The experiment lasted for eight weeks.The results showed as follows:1) the final weight,weight gain rate and specific growth rate were not significantly affected among groups(P>0.05).2) The superoxide dismutase activity in liver of 0.10% and 0.20% groups was significantly higher than that of control group(P<0.05),while the content of malondialdehyde in liver was significantly lower than that of control group(P<0.05).The catalase activity in liver of 0.10%,0.20%,0.40% and 0.80% groups was significantly higher than that of control group(P<0.05).The glutathione peroxidase activity and total antioxidant capacity in liver of 0.10% group were significantly higher than those of control group(P<0.05).3) The contents of complement 3 and complement 4 in serum of 0.10% group were significantly higher than those of control group(P<0.05).The lysozyme activity in serum of 0.10% and 0.20%groups was significantly higher than that of control group(P < 0.05).4) The content of serum glucose of0.10% and 0.20% groups was significantly lower than that of 0.05% group and control group(P<0.05).The content of serum cholesterol of 0.10%,0.20% and 0.40% groups was significantly lower than that of 0.05%group and control group(P<0.05).The content of serum triglyceride of 0.10% group was significantly lower than that of 0.05% group and control group(P < 0.05).The content of serum high density lipoprotein of0.10% and 0.20% groups was significantly higher than that of control group(P<0.05),and low density lipoprotein content was significantly lower than that of control group(P<0.05).In conclusion,dietary LBP can significantly improve antioxidant capacity and immunity of juvenile golden pompano,but not the growth.The suitable supplemental level of LBP in diets of juvenile golden pompano is 0.10%.
To study the effects of Lycium barbarum polysaccharide(LBP) on growth performance,antioxidant capacity,serum immune and biochemical indexes of juvenile golden pompano(Trachinotus ovatus),six diets were prepared with the addition of 0(control),0.05%,0.10%,0.20%,0.40% and 0.80% LBP,respectively.A total of 360 fish with an average body weight of(7.45±0.06) g were assigned randomly into six groups with three replicates per group and 20 fish per replicate.The experiment lasted for eight weeks.The results showed as follows:1) the final weight,weight gain rate and specific growth rate were not significantly affected among groups(P>0.05).2) The superoxide dismutase activity in liver of 0.10% and 0.20% groups was significantly higher than that of control group(P<0.05),while the content of malondialdehyde in liver was significantly lower than that of control group(P<0.05).The catalase activity in liver of 0.10%,0.20%,0.40% and 0.80% groups was significantly higher than that of control group(P<0.05).The glutathione peroxidase activity and total antioxidant capacity in liver of 0.10% group were significantly higher than those of control group(P<0.05).3) The contents of complement 3 and complement 4 in serum of 0.10% group were significantly higher than those of control group(P<0.05).The lysozyme activity in serum of 0.10% and 0.20%groups was significantly higher than that of control group(P < 0.05).4) The content of serum glucose of0.10% and 0.20% groups was significantly lower than that of 0.05% group and control group(P<0.05).The content of serum cholesterol of 0.10%,0.20% and 0.40% groups was significantly lower than that of 0.05%group and control group(P<0.05).The content of serum triglyceride of 0.10% group was significantly lower than that of 0.05% group and control group(P < 0.05).The content of serum high density lipoprotein of0.10% and 0.20% groups was significantly higher than that of control group(P<0.05),and low density lipoprotein content was significantly lower than that of control group(P<0.05).In conclusion,dietary LBP can significantly improve antioxidant capacity and immunity of juvenile golden pompano,but not the growth.The suitable supplemental level of LBP in diets of juvenile golden pompano is 0.10%.
引文
[1]AMAGASE H,FARNSWORTH N R.A reviewof botanical characteristics,phytochemistry,clinical relevance in efficacy and safety of Lycium barbarum fruit(Goji)[J].Food Research International,2011,44(7):1702-1717.
[2]王昌禄,王昵霏,李贞景.枸杞多糖的研究与应用[J].食品科学技术学报,2017,35(3):43-49.
[3]刘亚娟,陈赛娟,李海丽,等.枸杞多糖对生长獭兔生产性能及血清生化指标的影响[J].中国饲料,2013(20):22-25.
[4]尹瑞卿.枸杞多糖对肉仔鸡生产性能和免疫功能的调节作用[D].硕士学位论文.杨凌:西北农林科技大学,2013:24.
[5]陈赛娟,刘亚娟,王圆圆,等.枸杞多糖对泌乳母兔生产性能及血清生化指标的影响[J].中国农业科学,2013,46(10):2168-2174.
[6]王建东,马吉锋,李艳艳,等.不同浓度梯度枸杞多糖对小白鼠免疫功能的影响[J].黑龙江畜牧兽医,2016(7):207-209.
[7]孙晓雨,崔子寅,张明亮,等.枸杞多糖和茯苓多糖对免疫抑制小鼠免疫增强及对肠道黏膜的免疫调节作用[J].中国兽医学报,2015,35(3):450-455.
[8]王建东,马吉锋,李艳艳,等.枸杞多糖对动物免疫系统的影响[J].黑龙江畜牧兽医,2014(15):66-68.
[9]XIA G Q,XIN N,LIU W,et al.Inhibitory effect of Lycium barbarum polysaccharides on cell apoptosis and senescence is potentially mediated by the p53 signaling pathway[J].Molecular Medicine Reports,2014,9(4):1237-1241.
[10]XIN Y F,WAN L L,PENG J L,et al.Alleviation of the acute doxorubicin-induced cardiotoxicity by Lycium barbarum polysaccharides through the suppression of oxidative stress[J].Food and Chemical Toxicology,2011,49(1):259-264.
[11]龚涛,王晓辉,赵靓,等.枸杞多糖抗氧化作用的研究[J].生物技术,2010,20(1):84-86.
[12]丁园.枸杞多糖对2型糖尿病大鼠血糖、血脂的影响[J].辽宁医学院学报,2015,36(5):12-14.
[13]李朝晖,马晓鹂,吴万征.枸杞多糖降血糖作用的细胞实验研究[J].中药材,2012,35(1):124-127.
[14]徐赛华.枸杞多糖降脂作用的实验研究[J].海峡药学,2012,24(6):20-22.
[15]刘晴.枸杞多糖对CCl4诱导的大鼠肝纤维化的保护作用及机制研究[D].硕士学位论文.南京:南京农业大学,2016:16.
[16]吴旋.四种中草药多糖对黄颡鱼生长、体成分及部分生理生化指标的影响[D].硕士学位论文.天津:天津农学院,2011:1-2.
[17]白东清,吴旋,李玉华.枸杞多糖、灵芝多糖对金丝鱼生化指标的影响[J].中国饲料,2011(1):30-32.
[18]富丽静,宋文华,于翔,等.五种植物免疫增强剂对草鱼非特异免疫力的影响[J].水产学杂志,2010,23(4):14-17.
[19]王远吉.枸杞多糖对鲫鱼血清溶菌酶活性的影响[J].中国饲料,2004(24):28,31.
[20]刘楚斌,陈锤.卵形鲳鲹的生物学与养殖技术[J].齐鲁渔业,2009,26(6):32-33.
[21]杜强.卵形鲳鲹赖氨酸和蛋氨酸需求量及饲料中鱼粉替代的研究[D].硕士学位论文.上海:上海海洋大学,2012:12.
[22]OOI G T,TAWADROS N,ESCALONA R M.Pituitary cell lines and their endocrine applications[J].Molecular and Cellular Endocrinology,2004,228(1/2):1-21.
[23]THIEL L F,BEERMANN D H,KRICK B J,et al.Dose-dependent effects of exogenous porcine somatotropin on the yield,distribution,and proximate composition of carcass tissues in growing pigs[J].Journal of Animal Science,1993,71(4):827-835.
[24]赵世印,雷飞飞,谭华炳.枸杞多糖对非酒精性脂肪性肝病模型大鼠血脂代谢的影响[J].国际中医中药杂志,2015(4):331-334.
[25]孙克年.枸杞及枸杞多糖在水产养殖中的应用研究[J].广东饲料,2011,20(1):37-38.
[26]李宇敏,吴峰洋,冯佳婷,等.枸杞多糖的生物活性及其在动物生产中的应用研究[J].饲料研究,2017(22):22-25.
[27]LI X M,MA Y L,LIU X J.Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice[J].Journal of Ethnopharmacology,2007,111(3):504-511.
[28]刘杰,潘晓秋,周晖,等.枸杞多糖的提取及其抗衰老的研究[J].中国医院用药评价与分析,2016,16(3):357-359.
[29]艾庆辉,麦康森.鱼类营养免疫研究进展[J].水生生物学报,2007,31(3):425-430.
[30]余英才,张纯,夏循礼,等.补体系统的进化[J].生命科学,2012,24(4):362-367.
[31]马红樱,张德禄,胡春香,等.植物活性多糖的研究进展[J].西北师范大学学报(自然科学版),2004,40(3):112-117.
[32]韩娜娜,史成银.血液指标在鱼类学研究中的应用[J].安徽农业科学,2010,38(33):18877-18878,18880.
[33]杜强,林黑着,王芸,等.华南沿海5种海水鱼类血液生化指标的比较研究[J].饲料工业,2014,35(21):74-76.
[34]赵蕊,李青旺,高大威,等.枸杞多糖对Ⅱ型糖尿病小鼠胰岛细胞形态与功能的影响[J].燕山大学学报,2007,31(4):344-347.
[35]刘卫红,颜贤忠,张蕾,等.代谢组学技术在高脂血症及动脉粥样硬化痰瘀演变研究中的应用[J].辽宁中医杂志,2008,35(5):684-686.
[36]赵晶丽,高红梅,于海帅.枸杞多糖对高脂血症大鼠血脂代谢及氧自由基的影响[J].中国实验方剂学杂志,2013,19(5):241-243.
[37]MA M,LIU G H,YU Z H,et al.Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet in vivo[J].Food Chemistry,2009,113(4):872-877.
[38]陈玉春,顾雪飞,刘敏.5种中草药对鲤血清谷丙转氨酶、谷草转氨酶及红细胞过氧化氢酶活性的影响[J].淡水渔业,2007,37(5):11-13.
[2]王昌禄,王昵霏,李贞景.枸杞多糖的研究与应用[J].食品科学技术学报,2017,35(3):43-49.
[3]刘亚娟,陈赛娟,李海丽,等.枸杞多糖对生长獭兔生产性能及血清生化指标的影响[J].中国饲料,2013(20):22-25.
[4]尹瑞卿.枸杞多糖对肉仔鸡生产性能和免疫功能的调节作用[D].硕士学位论文.杨凌:西北农林科技大学,2013:24.
[5]陈赛娟,刘亚娟,王圆圆,等.枸杞多糖对泌乳母兔生产性能及血清生化指标的影响[J].中国农业科学,2013,46(10):2168-2174.
[6]王建东,马吉锋,李艳艳,等.不同浓度梯度枸杞多糖对小白鼠免疫功能的影响[J].黑龙江畜牧兽医,2016(7):207-209.
[7]孙晓雨,崔子寅,张明亮,等.枸杞多糖和茯苓多糖对免疫抑制小鼠免疫增强及对肠道黏膜的免疫调节作用[J].中国兽医学报,2015,35(3):450-455.
[8]王建东,马吉锋,李艳艳,等.枸杞多糖对动物免疫系统的影响[J].黑龙江畜牧兽医,2014(15):66-68.
[9]XIA G Q,XIN N,LIU W,et al.Inhibitory effect of Lycium barbarum polysaccharides on cell apoptosis and senescence is potentially mediated by the p53 signaling pathway[J].Molecular Medicine Reports,2014,9(4):1237-1241.
[10]XIN Y F,WAN L L,PENG J L,et al.Alleviation of the acute doxorubicin-induced cardiotoxicity by Lycium barbarum polysaccharides through the suppression of oxidative stress[J].Food and Chemical Toxicology,2011,49(1):259-264.
[11]龚涛,王晓辉,赵靓,等.枸杞多糖抗氧化作用的研究[J].生物技术,2010,20(1):84-86.
[12]丁园.枸杞多糖对2型糖尿病大鼠血糖、血脂的影响[J].辽宁医学院学报,2015,36(5):12-14.
[13]李朝晖,马晓鹂,吴万征.枸杞多糖降血糖作用的细胞实验研究[J].中药材,2012,35(1):124-127.
[14]徐赛华.枸杞多糖降脂作用的实验研究[J].海峡药学,2012,24(6):20-22.
[15]刘晴.枸杞多糖对CCl4诱导的大鼠肝纤维化的保护作用及机制研究[D].硕士学位论文.南京:南京农业大学,2016:16.
[16]吴旋.四种中草药多糖对黄颡鱼生长、体成分及部分生理生化指标的影响[D].硕士学位论文.天津:天津农学院,2011:1-2.
[17]白东清,吴旋,李玉华.枸杞多糖、灵芝多糖对金丝鱼生化指标的影响[J].中国饲料,2011(1):30-32.
[18]富丽静,宋文华,于翔,等.五种植物免疫增强剂对草鱼非特异免疫力的影响[J].水产学杂志,2010,23(4):14-17.
[19]王远吉.枸杞多糖对鲫鱼血清溶菌酶活性的影响[J].中国饲料,2004(24):28,31.
[20]刘楚斌,陈锤.卵形鲳鲹的生物学与养殖技术[J].齐鲁渔业,2009,26(6):32-33.
[21]杜强.卵形鲳鲹赖氨酸和蛋氨酸需求量及饲料中鱼粉替代的研究[D].硕士学位论文.上海:上海海洋大学,2012:12.
[22]OOI G T,TAWADROS N,ESCALONA R M.Pituitary cell lines and their endocrine applications[J].Molecular and Cellular Endocrinology,2004,228(1/2):1-21.
[23]THIEL L F,BEERMANN D H,KRICK B J,et al.Dose-dependent effects of exogenous porcine somatotropin on the yield,distribution,and proximate composition of carcass tissues in growing pigs[J].Journal of Animal Science,1993,71(4):827-835.
[24]赵世印,雷飞飞,谭华炳.枸杞多糖对非酒精性脂肪性肝病模型大鼠血脂代谢的影响[J].国际中医中药杂志,2015(4):331-334.
[25]孙克年.枸杞及枸杞多糖在水产养殖中的应用研究[J].广东饲料,2011,20(1):37-38.
[26]李宇敏,吴峰洋,冯佳婷,等.枸杞多糖的生物活性及其在动物生产中的应用研究[J].饲料研究,2017(22):22-25.
[27]LI X M,MA Y L,LIU X J.Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice[J].Journal of Ethnopharmacology,2007,111(3):504-511.
[28]刘杰,潘晓秋,周晖,等.枸杞多糖的提取及其抗衰老的研究[J].中国医院用药评价与分析,2016,16(3):357-359.
[29]艾庆辉,麦康森.鱼类营养免疫研究进展[J].水生生物学报,2007,31(3):425-430.
[30]余英才,张纯,夏循礼,等.补体系统的进化[J].生命科学,2012,24(4):362-367.
[31]马红樱,张德禄,胡春香,等.植物活性多糖的研究进展[J].西北师范大学学报(自然科学版),2004,40(3):112-117.
[32]韩娜娜,史成银.血液指标在鱼类学研究中的应用[J].安徽农业科学,2010,38(33):18877-18878,18880.
[33]杜强,林黑着,王芸,等.华南沿海5种海水鱼类血液生化指标的比较研究[J].饲料工业,2014,35(21):74-76.
[34]赵蕊,李青旺,高大威,等.枸杞多糖对Ⅱ型糖尿病小鼠胰岛细胞形态与功能的影响[J].燕山大学学报,2007,31(4):344-347.
[35]刘卫红,颜贤忠,张蕾,等.代谢组学技术在高脂血症及动脉粥样硬化痰瘀演变研究中的应用[J].辽宁中医杂志,2008,35(5):684-686.
[36]赵晶丽,高红梅,于海帅.枸杞多糖对高脂血症大鼠血脂代谢及氧自由基的影响[J].中国实验方剂学杂志,2013,19(5):241-243.
[37]MA M,LIU G H,YU Z H,et al.Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet in vivo[J].Food Chemistry,2009,113(4):872-877.
[38]陈玉春,顾雪飞,刘敏.5种中草药对鲤血清谷丙转氨酶、谷草转氨酶及红细胞过氧化氢酶活性的影响[J].淡水渔业,2007,37(5):11-13.