培养基中添加海藻糖对大球盖菇、斑玉蕈菌丝生长的影响
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摘要
【背景】大球盖菇和斑玉蕈是食药兼用且具有开发潜力的珍稀食用菌,培养基对菌丝生长及子实体发育具有重要作用,优化培养基显得尤为重要。【目的】筛选出最适合培养大球盖菇、斑玉蕈的新型培养基。【方法】使用添加海藻糖的新型培养基,对不同培养基培养的大球盖菇及斑玉蕈菌株的菌丝生长状况、生长速度和生物量及纤维素酶、漆酶活性进行测定与分析。【结果】相较于PDA培养基,添加海藻糖的培养基能够提高菌丝生长速度、增加生物量,海藻糖添加的比例对纤维素酶和漆酶的影响较大,对大球盖菇及斑玉蕈菌丝的生长产生显著的促进作用,但不会改变其蛋白质的组成。【结论】大球盖菇最适合选用PTA-5培养基,斑玉蕈的最佳培养基是PDTA培养基。
[Background] Stropharia rugosoannulata and Hypsizigus marmoreus are rare edible and medicinal fungi with great development potential. The medium plays an important role in mycelial growth and fruit body development. Optimizing the medium of the two mushroom is particularly important.[Objective] Screen out the new medium more suitable for S. rugosoannulata and H. marmoreus cultivation.[Methods] Using new media containing trehalose for the cultivation of S. rugosoannulata and H.marmoreus. The mycelial growth status, growth rate and biomass, cellulase and laccase activities were measured and analyzed in different culture media. [Results] Trehalose-added media promoted the growth rate of mycelia and increased the biomass compared with PDA medium. The proportion of trehalose content had a great effect on cellulase and laccase activity. Trehalose-added media could significantly promote the growth of hyphae, but the protein composition did not change. [Conclusion] PTA-5 was more suitable for the cultivation of S. rugosoannulata mycelia; PDTA was more suitable for the cultivation of H. marmoreus mycelia.
[Background] Stropharia rugosoannulata and Hypsizigus marmoreus are rare edible and medicinal fungi with great development potential. The medium plays an important role in mycelial growth and fruit body development. Optimizing the medium of the two mushroom is particularly important.[Objective] Screen out the new medium more suitable for S. rugosoannulata and H. marmoreus cultivation.[Methods] Using new media containing trehalose for the cultivation of S. rugosoannulata and H.marmoreus. The mycelial growth status, growth rate and biomass, cellulase and laccase activities were measured and analyzed in different culture media. [Results] Trehalose-added media promoted the growth rate of mycelia and increased the biomass compared with PDA medium. The proportion of trehalose content had a great effect on cellulase and laccase activity. Trehalose-added media could significantly promote the growth of hyphae, but the protein composition did not change. [Conclusion] PTA-5 was more suitable for the cultivation of S. rugosoannulata mycelia; PDTA was more suitable for the cultivation of H. marmoreus mycelia.
引文
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[13]Zhang RY,Hu DD,Zuo XM,et al.Advances in the preservation of edible mushroom cultures[J].Acta Edulis Fungi,2010,17(4):84-88(in Chinese)张瑞颖,胡丹丹,左雪梅,等.食用菌菌种保藏技术研究进展[J].食用菌学报,2010,17(4):84-88
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[15]Zhang XL,Chen XL,Li H.Determination of enzymatic properties of a laccase Lac1338,and effects of directed mutants on the degradations of different dyes[J].Biotechnology Bulletin,2016,32(7):170-177(in Chinese)张雪玲,陈小利,李荷.漆酶Lac1338的酶学特性测定及定向突变对其酶解染料影响[J].生物技术通报,2016,32(7):170-177
[16]Olsson C,Jansson H,Swenson J.The role of trehalose for the stabilization of proteins[J].The Journal of Physical Chemistry B,2016,120(20):4723-4731
[17]Hottiger T,de Virgilio C,Hall MN,et al.The role of trehalose synthesis for the acquisition of thermotolerance in yeast.II.Physiological concentrations of trehalose increase the thermal stability of proteins in vitro[J].The FEBS Journal,1994,219(1/2):187-193
[18]Bhaganna P,Volkers RJM,Bell ANW,et al.Hydrophobic substances induce water stress in microbial cells[J].Microbial Biotechnology,2010,3(6):701-716
[19]Kar JR,Hallsworth JE,Singhal RS.Fermentative production of glycine betaine and trehalose from acid whey using Actinopolyspora halophila(MTCC 263)[J].Environmental Technology&Innovation,2015,3:68-76
[20]Zhang JJ,Chen MJ,Feng ZY,et al.Effects of aromatic compounds on biomass,laccase activities and transcript levels of Hypsizygus marmoreus[J].Mycosystema,2016,35(9):1130-1138(in Chinese)张津京,陈明杰,冯志勇,等.芳香族化合物对斑玉蕈菌丝生物量、漆酶活性及其转录水平的影响[J].菌物学报,2016,35(9):1130-1138
[21]Rubio-Texeira M,van Zeebroeck G,Thevelein JM.Trehalose metabolism:enzymatic pathways and physiological functions[A]//Hoffmeister D.Biochemistry and Molecular Biology[M].Cham:Springer,2016:191-277
[22]Zhao X,Song XX,Li YP,et al.Gene expression related to trehalose metabolism and its effect on Volvariella volvacea under low temperature stress[J].Scientific Reports,2018,8(1):11011
[2]Zhang J,Zhang GQ,Zou L,et al.Isolation,purification and ITSsequence identification of Stropharia Rugosoannulata[J].Jiangsu Agricultural Sciences,2017,45(1):120-123(in Chinese)张健,张国权,邹莉,等.大球盖菇的分离纯化及ITS序列鉴定[J].江苏农业科学,2017,45(1):120-123
[3]Chen H,Feng C,Chen MJ,et al.Effects of exogenous saponin on the growth and development of Hypsizygus marmoreus[J].Acta Agriculturae Shanghai,2017,33(3):31-36(in Chinese)陈辉,冯川,陈明杰,等.外源添加皂苷对斑玉蕈生长发育的影响[J].上海农业学报,2017,33(3):31-36
[4]Hu KH,Liu JZ,Sun SJ,et al.Laccase transtormation system established in Hypsizigus marmoreus breeding[J].Mycosystema,2010,29(4):528-535(in Chinese)胡开辉,刘建忠,孙淑静,等.斑玉蕈育种中漆酶转化体系建立的初步研究[J].菌物学报,2010,29(4):528-535
[5]Zhang D,Yan MQ,Liu JY,et al.Influence factors on short-and medium-term spawn preservation of Flammulina velutipes using orthogonal experimental design[J].Microbiology China,2018,45(6):1375-1383(in Chinese)张丹,颜梦秋,刘建雨,等.金针菇菌种中短期保藏因素的正交分析[J].微生物学通报,2018,45(6):1375-1383
[6]Wu XZ,Wang QL,Du ST,et al.Effect of several different inorganic salts,vitamins and plant hormones on the mycelial growth of Hypsizigus mermoreus(Peck)bigelow[J].Journal of Northwest A&F University(Natural Science Edition),2012,40(3):158-162(in Chinese)吴秀珍,王秋玲,杜双田,等.无机盐、维生素及生长调节剂对斑玉蕈菌丝生长的影响[J].西北农林科技大学学报:自然科学版,2012,40(3):158-162
[7]Guo YY,Ruan LY,Feng HC,et al.Characteristics of mycelial growth and enzyme production of Hypsizygus marmoreus under different nutritional conditions[J].Mycosystema,2014,33(3):697-705(in Chinese)郭艳艳,阮玲云,冯宏昌,等.不同营养条件下斑玉蕈菌丝生长及产酶特性[J].菌物学报,2014,33(3):697-705
[8]Rangel DEN,Braga GUL,FernandeséKK,et al.Stress tolerance and virulence of insect-pathogenic fungi are determined by environmental conditions during conidial formation[J].Current Genetics,2015,61(3):383-404
[9]Paul MJ,Gonzalez-Uriarte A,Griffiths CA,et al.The role of trehalose 6-phosphate in crop yield and resilience[J].Plant Physiology,2018,177(1):12-23
[10]Sun M.Mycelium culture and the variation regularity of extracellular enzyme activity of Stropharia rugosoannulata Farlow[D].Yanji:Master’s Thesis of Yanbian University,2013(in Chinese)孙萌.大球盖菇菌丝培养及胞外酶活性变化规律研究[D].延吉:延边大学硕士学位论文,2013
[11]Zhang JJ.The functions of laccase on the mycelial growth and fruiting body development of basibiomycete Hypsizigus marmoreus[D].Nanjing:Doctoral Dissertation of Nanjing Agricultural University,2014(in Chinese)张津京.漆酶在斑玉蕈菌丝生长和子实体发育中的功能研究[D].南京:南京农业大学博士学位论文,2014
[12]Zhu HX.The comparison for the capacity of laccase production in various types of edible fungi and the characterization of laccase[D].Fuzhou:Master’s Thesis of Fujian Agriculture and Forestry University,2008(in Chinese)朱海潇.食用菌产漆酶能力的比较及漆酶性能的研究[D].福州:福建农林大学硕士学位论文,2008
[13]Zhang RY,Hu DD,Zuo XM,et al.Advances in the preservation of edible mushroom cultures[J].Acta Edulis Fungi,2010,17(4):84-88(in Chinese)张瑞颖,胡丹丹,左雪梅,等.食用菌菌种保藏技术研究进展[J].食用菌学报,2010,17(4):84-88
[14]Mandels M,Hontz L,Nystrom J,et al.Enzymatic hydrolysis of waste cellulose[J].Biotechnology and Bioengineering,2010,105(1):1-25
[15]Zhang XL,Chen XL,Li H.Determination of enzymatic properties of a laccase Lac1338,and effects of directed mutants on the degradations of different dyes[J].Biotechnology Bulletin,2016,32(7):170-177(in Chinese)张雪玲,陈小利,李荷.漆酶Lac1338的酶学特性测定及定向突变对其酶解染料影响[J].生物技术通报,2016,32(7):170-177
[16]Olsson C,Jansson H,Swenson J.The role of trehalose for the stabilization of proteins[J].The Journal of Physical Chemistry B,2016,120(20):4723-4731
[17]Hottiger T,de Virgilio C,Hall MN,et al.The role of trehalose synthesis for the acquisition of thermotolerance in yeast.II.Physiological concentrations of trehalose increase the thermal stability of proteins in vitro[J].The FEBS Journal,1994,219(1/2):187-193
[18]Bhaganna P,Volkers RJM,Bell ANW,et al.Hydrophobic substances induce water stress in microbial cells[J].Microbial Biotechnology,2010,3(6):701-716
[19]Kar JR,Hallsworth JE,Singhal RS.Fermentative production of glycine betaine and trehalose from acid whey using Actinopolyspora halophila(MTCC 263)[J].Environmental Technology&Innovation,2015,3:68-76
[20]Zhang JJ,Chen MJ,Feng ZY,et al.Effects of aromatic compounds on biomass,laccase activities and transcript levels of Hypsizygus marmoreus[J].Mycosystema,2016,35(9):1130-1138(in Chinese)张津京,陈明杰,冯志勇,等.芳香族化合物对斑玉蕈菌丝生物量、漆酶活性及其转录水平的影响[J].菌物学报,2016,35(9):1130-1138
[21]Rubio-Texeira M,van Zeebroeck G,Thevelein JM.Trehalose metabolism:enzymatic pathways and physiological functions[A]//Hoffmeister D.Biochemistry and Molecular Biology[M].Cham:Springer,2016:191-277
[22]Zhao X,Song XX,Li YP,et al.Gene expression related to trehalose metabolism and its effect on Volvariella volvacea under low temperature stress[J].Scientific Reports,2018,8(1):11011