土荆芥挥发油对幽门螺杆菌甲硝唑耐药株及敏感株的体外抗菌作用研究
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摘要
目的研究土荆芥挥发油对幽门螺杆菌(HP)甲硝唑耐药株及敏感株的体外抗菌作用。方法选取HP甲硝唑耐药株(26695-16R、26695-8R16S、26695-2R8S)、敏感株(NCTC 11637、26695)作为实验菌株,采用倍比稀释法观察土荆芥挥发油对HP在不同浓度、不同作用时间下生长情况的影响,并测定其最低抑菌浓度(MIC)。结果土荆芥挥发油在不同时间情况下对HP甲硝唑耐药株及敏感株的MIC值为4～256μg/m L不等,其差异性随药物作用时间的延长而降低。结论土荆芥挥发油对HP甲硝唑耐药株及敏感株均具有明确的抗菌作用,其抗菌效能与药物剂量呈正相关,并于72 h后趋向稳定。
Objective To study the bacteriostasis of Chenopodium ambrosioides L. against HP metronidazole resistant and susceptible strains in vitro. Methods The minimal inhibitory concentrations (MIC) of Chenopodium ambrosioides L. against HP were selected,agar dilution method used to observe the effect of the drug on the growth of HP under different drug concentrations and different action times,with HP metronidazole resistant strain (26695-16R,26695-8 R16S,26695-2 R8S) and susceptible strains (NCTC 11637,26695)setting as the experimental strains. Results Chenopodium ambrosioides L. was active against HP metronidazole resistant and susceptible strains under different action times (MIC value 4 ～ 256 μg/m L),and the differences of MIC values decreased with the prolongation of drug action time. Conclusion Chenopodium ambrosioides L. had bacteriostasis to HP metronidazole resistant and susceptible strains. The antibacterial effect was positively related with the drug concentrations and the effect tended to be stable after 72 h.
Objective To study the bacteriostasis of Chenopodium ambrosioides L. against HP metronidazole resistant and susceptible strains in vitro. Methods The minimal inhibitory concentrations (MIC) of Chenopodium ambrosioides L. against HP were selected,agar dilution method used to observe the effect of the drug on the growth of HP under different drug concentrations and different action times,with HP metronidazole resistant strain (26695-16R,26695-8 R16S,26695-2 R8S) and susceptible strains (NCTC 11637,26695)setting as the experimental strains. Results Chenopodium ambrosioides L. was active against HP metronidazole resistant and susceptible strains under different action times (MIC value 4 ～ 256 μg/m L),and the differences of MIC values decreased with the prolongation of drug action time. Conclusion Chenopodium ambrosioides L. had bacteriostasis to HP metronidazole resistant and susceptible strains. The antibacterial effect was positively related with the drug concentrations and the effect tended to be stable after 72 h.
引文
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[2]刘伟,张学智,李宁,等.土荆芥提取物与克拉霉素或甲硝唑联用对幽门螺杆菌杀菌作用的研究[J].中国中西医结合消化杂志,2011,19(5):281-283.
[3]Liu W,Liu Y,Zhang XZ,et al.In vitro bactericidal activity of Jinghua Weikang Capsule and its individual herb Chenopodium ambrosioides L.against antibioticresistant Helicobacter pylori[J].Chinese Journal of Integrative Medicine,2013,19(1):54.
[4]胡伏莲,成虹,张学智,等.多中心临床观察荆花胃康联合三联疗法治疗幽门螺杆菌相关性十二指肠溃疡和胃炎疗效及耐药分析[J].中华医学杂志,2012,92(10):679-684.
[5]张月苗,王婷婷,叶晖,等.荆花胃康胶丸联合三联疗法治疗幽门螺杆菌感染慢性胃炎疗效观察[J].中国中西医结合消化杂志,2013,21(11):587-590.
[6]王婷婷,张月苗,张学智,等.荆花胃康胶丸联合PPI三联疗法对幽门螺杆菌阳性慢性萎缩性胃炎的效果:多中心随机对照临床研究[J].中华医学杂志,2013,93(44):3491-3495.
[7]Ye H,Liu Y,Li N,et al.Anti-Helicobacter pylori activities of Chenopodium ambrosioides L.in vitro and in vivo[J].World Journal of Gastroenterology,2015,21(14):4178-4183.